Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers
The aim of this study was to assess the effect of local application of essential oil of <i>Pistacia atlantica</i> kurdica (EOK) gel in treatment of experimentally induced periodontitis in rats and its effect on osteoclastogenic bone markers. Twenty-four male Wistar rats of 250 to 350 g w...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/24/5819 |
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| author | Shokhan H. Azeez Shanaz M. Gaphor Aram M. Sha Balkees T. Garib |
| author_facet | Shokhan H. Azeez Shanaz M. Gaphor Aram M. Sha Balkees T. Garib |
| author_sort | Shokhan H. Azeez |
| collection | DOAJ |
| description | The aim of this study was to assess the effect of local application of essential oil of <i>Pistacia atlantica</i> kurdica (EOK) gel in treatment of experimentally induced periodontitis in rats and its effect on osteoclastogenic bone markers. Twenty-four male Wistar rats of 250 to 350 g were used in this study and were allocated into four groups. Control negative (without induced periodontitis), control positive (induced experimental periodontitis left without treatment), treatment control (induced experimental periodontitis and treated with Chlorhexidine gel) and EOK treated group (induced experimental periodontitis treated with EOK gel). The animals were sacrificed after 30 days, and the mandibular central incisor and surrounding tissue were dissected from the mandible and further processed for preparing H&E slides. Inflammatory cells, osteoclast cells, and periodontal ligament (PDL) were examined and measured histologically. Finally, the mean concentrations of both markers, receptor activator of nuclear factor kappa-Β ligand (RANKL) and (Interleukin-1β) IL-1β, were analyzed by ELISA. A significant reduction of inflammatory reaction and osteoclast numbers with improvement of PDL and low mean concentrations of RANKL and IL-1β were seen in the EOK treated group in comparison to the control group and the chlorhexidine group as well. The extract showed a protective effect in the healing of periodontitis that had been induced in rats and decreased bone resorption by down regulation of serum RANKL and IL-1β markers. |
| first_indexed | 2024-03-10T14:12:10Z |
| format | Article |
| id | doaj.art-dca14ee81fd349fba11bc281ba8fc499 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:12:10Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dca14ee81fd349fba11bc281ba8fc4992023-11-21T00:07:13ZengMDPI AGMolecules1420-30492020-12-012524581910.3390/molecules25245819Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone MarkersShokhan H. Azeez0Shanaz M. Gaphor1Aram M. Sha2Balkees T. Garib3Department of Dental Nursing, Sulaimani Technical Institute, Sulaimani Polytechnic University, Sulaimani 46001, Kurdistan, IraqDepartment of Oral Diagnosis, College of Dentistry, University of Sulaimani, Sulaimani 46001, Kurdistan, IraqPeriodontics Department, College of Dentistry, University of Sulaimani, Sulaimani 46001, Kurdistan, IraqDepartment of Oral Diagnosis, College of Dentistry, University of Sulaimani, Sulaimani 46001, Kurdistan, IraqThe aim of this study was to assess the effect of local application of essential oil of <i>Pistacia atlantica</i> kurdica (EOK) gel in treatment of experimentally induced periodontitis in rats and its effect on osteoclastogenic bone markers. Twenty-four male Wistar rats of 250 to 350 g were used in this study and were allocated into four groups. Control negative (without induced periodontitis), control positive (induced experimental periodontitis left without treatment), treatment control (induced experimental periodontitis and treated with Chlorhexidine gel) and EOK treated group (induced experimental periodontitis treated with EOK gel). The animals were sacrificed after 30 days, and the mandibular central incisor and surrounding tissue were dissected from the mandible and further processed for preparing H&E slides. Inflammatory cells, osteoclast cells, and periodontal ligament (PDL) were examined and measured histologically. Finally, the mean concentrations of both markers, receptor activator of nuclear factor kappa-Β ligand (RANKL) and (Interleukin-1β) IL-1β, were analyzed by ELISA. A significant reduction of inflammatory reaction and osteoclast numbers with improvement of PDL and low mean concentrations of RANKL and IL-1β were seen in the EOK treated group in comparison to the control group and the chlorhexidine group as well. The extract showed a protective effect in the healing of periodontitis that had been induced in rats and decreased bone resorption by down regulation of serum RANKL and IL-1β markers.https://www.mdpi.com/1420-3049/25/24/5819bone lossexperimental periodontitisosteoclasts<i>Pistacia atlantica</i> subsp. kurdicaPorphyromonas gingivalis |
| spellingShingle | Shokhan H. Azeez Shanaz M. Gaphor Aram M. Sha Balkees T. Garib Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers Molecules bone loss experimental periodontitis osteoclasts <i>Pistacia atlantica</i> subsp. kurdica Porphyromonas gingivalis |
| title | Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers |
| title_full | Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers |
| title_fullStr | Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers |
| title_full_unstemmed | Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers |
| title_short | Effect of <i>Pistacia atlantica</i> subsp. kurdica Gum in Experimental Periodontitis Induced in Wistar Rats by Utilization of Osteoclastogenic Bone Markers |
| title_sort | effect of i pistacia atlantica i subsp kurdica gum in experimental periodontitis induced in wistar rats by utilization of osteoclastogenic bone markers |
| topic | bone loss experimental periodontitis osteoclasts <i>Pistacia atlantica</i> subsp. kurdica Porphyromonas gingivalis |
| url | https://www.mdpi.com/1420-3049/25/24/5819 |
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