Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, Tanzania
In Tanzania, information on antimicrobial resistance in small-scale dairy cattle is scarce. This cross-sectional study was conducted to determine the different levels and pattern of antimicrobial resistance (AMR), in 121 <i>Escherichia coli</i> isolated from rectal swab of 201 apparently...
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MDPI AG
2022-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/14/1853 |
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| author | Rogers R. Azabo Stephen E. Mshana Mecky I. Matee Sharadhuli I. Kimera |
| author_facet | Rogers R. Azabo Stephen E. Mshana Mecky I. Matee Sharadhuli I. Kimera |
| author_sort | Rogers R. Azabo |
| collection | DOAJ |
| description | In Tanzania, information on antimicrobial resistance in small-scale dairy cattle is scarce. This cross-sectional study was conducted to determine the different levels and pattern of antimicrobial resistance (AMR), in 121 <i>Escherichia coli</i> isolated from rectal swab of 201 apparently healthy small-scale dairy cattle in Dar es Salaam, Tanzania. Isolation and identification of <i>E. coli</i> were carried out using enrichment media, selective media, and biochemical tests. Antimicrobial susceptibility testing was carried out using the Kirby–Bauer disk diffusion method on Mueller-Hinton agar (Merck), according to the recommendations of Clinical and Laboratory Standards Institute (CLSI). Resistance was tested against ampicillin, gentamicin, chloramphenicol, trimethoprim-sulfamethoxazole, tetracycline, nalidixic acid, ciprofloxacin and cefotaxime. Resistance to almost all antimicrobial agents was observed. The agents to which resistance was demonstrated most frequently were ampicillin (96.7%), cefotaxime (95.0%), tetracycline (50.4%), trimethoprim-sulfamethoxazole (42.1%) and nalidixic acid (33.1%). In this case, 20 extended-spectrum beta-lactamases (ESBLs) producing <i>E. coli</i> were identified. 74.4% (90/121) of the isolates were Multidrug resistant (MDR), ranging from a combination of three to 8 different classes. The most frequently observed phenotypes were AMP-SXT-CTX with a prevalence of 12.4%, followed by the combination AMP-CTX with 10.7% and TE-AMP-CTX and NA + TE + AMP + CTX with 8.3% each. The high prevalence and wide range of AMR calls for prudent antimicrobial use. |
| first_indexed | 2024-03-09T03:47:53Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-09T03:47:53Z |
| publishDate | 2022-07-01 |
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| series | Animals |
| spelling | doaj.art-dca92c95e28e4b4085208955073444412023-12-03T14:32:13ZengMDPI AGAnimals2076-26152022-07-011214185310.3390/ani12141853Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, TanzaniaRogers R. Azabo0Stephen E. Mshana1Mecky I. Matee2Sharadhuli I. Kimera3Department of Veterinary Microbiology, Parasitology and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P.O. Box 3019, Morogoro 67125, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza 33109, TanzaniaSACIDS Africa Centre of Excellence for Infectious Diseases, Sokoine University of Agriculture, P.O. Box 3297, Morogoro 67125, TanzaniaSACIDS Africa Centre of Excellence for Infectious Diseases, Sokoine University of Agriculture, P.O. Box 3297, Morogoro 67125, TanzaniaIn Tanzania, information on antimicrobial resistance in small-scale dairy cattle is scarce. This cross-sectional study was conducted to determine the different levels and pattern of antimicrobial resistance (AMR), in 121 <i>Escherichia coli</i> isolated from rectal swab of 201 apparently healthy small-scale dairy cattle in Dar es Salaam, Tanzania. Isolation and identification of <i>E. coli</i> were carried out using enrichment media, selective media, and biochemical tests. Antimicrobial susceptibility testing was carried out using the Kirby–Bauer disk diffusion method on Mueller-Hinton agar (Merck), according to the recommendations of Clinical and Laboratory Standards Institute (CLSI). Resistance was tested against ampicillin, gentamicin, chloramphenicol, trimethoprim-sulfamethoxazole, tetracycline, nalidixic acid, ciprofloxacin and cefotaxime. Resistance to almost all antimicrobial agents was observed. The agents to which resistance was demonstrated most frequently were ampicillin (96.7%), cefotaxime (95.0%), tetracycline (50.4%), trimethoprim-sulfamethoxazole (42.1%) and nalidixic acid (33.1%). In this case, 20 extended-spectrum beta-lactamases (ESBLs) producing <i>E. coli</i> were identified. 74.4% (90/121) of the isolates were Multidrug resistant (MDR), ranging from a combination of three to 8 different classes. The most frequently observed phenotypes were AMP-SXT-CTX with a prevalence of 12.4%, followed by the combination AMP-CTX with 10.7% and TE-AMP-CTX and NA + TE + AMP + CTX with 8.3% each. The high prevalence and wide range of AMR calls for prudent antimicrobial use.https://www.mdpi.com/2076-2615/12/14/1853antimicrobial resistancecattle<i>Escherichia coli</i>Dar es SalaamTanzania |
| spellingShingle | Rogers R. Azabo Stephen E. Mshana Mecky I. Matee Sharadhuli I. Kimera Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, Tanzania Animals antimicrobial resistance cattle <i>Escherichia coli</i> Dar es Salaam Tanzania |
| title | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, Tanzania |
| title_full | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, Tanzania |
| title_fullStr | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, Tanzania |
| title_full_unstemmed | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, Tanzania |
| title_short | Antimicrobial Resistance Pattern of <i>Escherichia coli</i> Isolates from Small Scale Dairy Cattle in Dar es Salaam, Tanzania |
| title_sort | antimicrobial resistance pattern of i escherichia coli i isolates from small scale dairy cattle in dar es salaam tanzania |
| topic | antimicrobial resistance cattle <i>Escherichia coli</i> Dar es Salaam Tanzania |
| url | https://www.mdpi.com/2076-2615/12/14/1853 |
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