Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.)
The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2010-03-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201001-0004_antioxidant-enzymes-changes-in-response-to-drought-stress-in-ten-cultivars-of-oilseed-rape-brassica-napus-l.php |
| _version_ | 1797899506870648832 |
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| author | Tayebeh ABEDI Hassan PAKNIYAT |
| author_facet | Tayebeh ABEDI Hassan PAKNIYAT |
| author_sort | Tayebeh ABEDI |
| collection | DOAJ |
| description | The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress. |
| first_indexed | 2024-04-10T08:29:54Z |
| format | Article |
| id | doaj.art-dcacba66429d4d83b6560da8c97d14b5 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:54Z |
| publishDate | 2010-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-dcacba66429d4d83b6560da8c97d14b52023-02-23T03:30:07ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252010-03-01461273410.17221/67/2009-CJGPBcjg-201001-0004Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.)Tayebeh ABEDI0Hassan PAKNIYAT1Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, IranDepartment of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, IranThe study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape (Brassica napus L.) cultivars under drought stress conditions. Plants were grown under three irrigation regimes (FC; field capacity, 60% FC and 30% FC) in a greenhouse. Drought stress preferentially enhanced the activities of superoxide dismutase (SOD) and guaiacol peroxidase (POD) whereas it decreased catalase (CAT) activity. Licord with the highest level of enzyme activity under both optimum and limited irrigation regimes is reported as the most tolerant cultivar. Whereas Hyola 308 and Okapy, having the lowest enzymes activities, are mentioned as cultivars sensitive to drought stress. The native polyacrylamide gel electrophoresis (PAGE) analysis detected eight SOD isozymes. Oilseed rape leaves contained three isoforms of Mn-SOD and five isoforms of Cu/Zn-SOD. The expression of Mn-SOD was preferentially enhanced by drought stress. Five POD isoforms were detected in oilseed rape leaves. The intensities of POD-4 and -5 were enhanced under drought stress. According to the results, the appearance of new isozyme bands under drought stress conditions may be used as a biochemical marker to differentiate drought tolerant cultivars under drought stress.https://cjgpb.agriculturejournals.cz/artkey/cjg-201001-0004_antioxidant-enzymes-changes-in-response-to-drought-stress-in-ten-cultivars-of-oilseed-rape-brassica-napus-l.phpcatalaseguaiacol peroxidaseisozymesoilseed rapesuperoxide dismutasewater stress |
| spellingShingle | Tayebeh ABEDI Hassan PAKNIYAT Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.) Czech Journal of Genetics and Plant Breeding catalase guaiacol peroxidase isozymes oilseed rape superoxide dismutase water stress |
| title | Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.) |
| title_full | Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.) |
| title_fullStr | Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.) |
| title_full_unstemmed | Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.) |
| title_short | Antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.) |
| title_sort | antioxidant enzymes changes in response to drought stress in ten cultivars of oilseed rape brassica napus l |
| topic | catalase guaiacol peroxidase isozymes oilseed rape superoxide dismutase water stress |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201001-0004_antioxidant-enzymes-changes-in-response-to-drought-stress-in-ten-cultivars-of-oilseed-rape-brassica-napus-l.php |
| work_keys_str_mv | AT tayebehabedi antioxidantenzymeschangesinresponsetodroughtstressintencultivarsofoilseedrapebrassicanapusl AT hassanpakniyat antioxidantenzymeschangesinresponsetodroughtstressintencultivarsofoilseedrapebrassicanapusl |