MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents
Photochemoprevention can be a valuable approach to counteract the damaging effects of environmental stressors (e.g., UV radiations) on the skin. Pigments are bioactive molecules, greatly attractive for biotechnological purposes, and with promising applications for human health. In this context, mari...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/7/354 |
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| author | Luigi Pistelli Clementina Sansone Arianna Smerilli Marco Festa Douglas M. Noonan Adriana Albini Christophe Brunet |
| author_facet | Luigi Pistelli Clementina Sansone Arianna Smerilli Marco Festa Douglas M. Noonan Adriana Albini Christophe Brunet |
| author_sort | Luigi Pistelli |
| collection | DOAJ |
| description | Photochemoprevention can be a valuable approach to counteract the damaging effects of environmental stressors (e.g., UV radiations) on the skin. Pigments are bioactive molecules, greatly attractive for biotechnological purposes, and with promising applications for human health. In this context, marine microalgae are a valuable alternative and eco-sustainable source of pigments that still need to be taken advantage of. In this study, a comparative in vitro photochemopreventive effects of twenty marine pigments on carcinogenic melanoma model cell B16F0 from UV-induced injury was setup. Pigment modulation of the intracellular reactive oxygen species (ROS) concentration and extracellular release of nitric oxide (NO) was investigated. At the cell signaling level, interleukin 1-β (IL-1β) and matrix metallopeptidase 9 protein (MMP-9) protein expression was examined. These processes are known to be involved in the signaling pathway, from UV stress to cancer induction. Diatoxanthin resulted the best performing pigment in lowering MMP-9 levels and was able to strongly lower IL-1β. This study highlights the pronounced bioactivity of the exclusively aquatic carotenoid diatoxanthin, among the others. It is suggested increasing research efforts on this molecule, emphasizing that a deeper integration of plant ecophysiological studies into a biotechnological context could improve the exploration and exploitation of bioactive natural products. |
| first_indexed | 2024-03-10T10:11:08Z |
| format | Article |
| id | doaj.art-dcb0463487e942cbb1adcf3826d0459c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T10:11:08Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-dcb0463487e942cbb1adcf3826d0459c2023-11-22T01:12:19ZengMDPI AGMarine Drugs1660-33972021-06-0119735410.3390/md19070354MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective AgentsLuigi Pistelli0Clementina Sansone1Arianna Smerilli2Marco Festa3Douglas M. Noonan4Adriana Albini5Christophe Brunet6Stazione Zoologica Anton Dohrn, Istituto Nazionale di Biologia, Ecologia e Biotecnologie Marine, Villa Comunale, 80121 Napoli, ItalyStazione Zoologica Anton Dohrn, Istituto Nazionale di Biologia, Ecologia e Biotecnologie Marine, Villa Comunale, 80121 Napoli, ItalyStazione Zoologica Anton Dohrn, Istituto Nazionale di Biologia, Ecologia e Biotecnologie Marine, Villa Comunale, 80121 Napoli, ItalyLaboratory of Vascular Biology and Angiogenesis, IRCCS MultiMedica, 20138 Milan, ItalyDepartment of Biotechnology and Life Sciences, University of Insubria, 21100 Varese, ItalyLaboratory of Vascular Biology and Angiogenesis, IRCCS MultiMedica, 20138 Milan, ItalyStazione Zoologica Anton Dohrn, Istituto Nazionale di Biologia, Ecologia e Biotecnologie Marine, Villa Comunale, 80121 Napoli, ItalyPhotochemoprevention can be a valuable approach to counteract the damaging effects of environmental stressors (e.g., UV radiations) on the skin. Pigments are bioactive molecules, greatly attractive for biotechnological purposes, and with promising applications for human health. In this context, marine microalgae are a valuable alternative and eco-sustainable source of pigments that still need to be taken advantage of. In this study, a comparative in vitro photochemopreventive effects of twenty marine pigments on carcinogenic melanoma model cell B16F0 from UV-induced injury was setup. Pigment modulation of the intracellular reactive oxygen species (ROS) concentration and extracellular release of nitric oxide (NO) was investigated. At the cell signaling level, interleukin 1-β (IL-1β) and matrix metallopeptidase 9 protein (MMP-9) protein expression was examined. These processes are known to be involved in the signaling pathway, from UV stress to cancer induction. Diatoxanthin resulted the best performing pigment in lowering MMP-9 levels and was able to strongly lower IL-1β. This study highlights the pronounced bioactivity of the exclusively aquatic carotenoid diatoxanthin, among the others. It is suggested increasing research efforts on this molecule, emphasizing that a deeper integration of plant ecophysiological studies into a biotechnological context could improve the exploration and exploitation of bioactive natural products.https://www.mdpi.com/1660-3397/19/7/354diatoxanthinalloxanthinoxidative stresscarotenoidsanti-inflammationROS |
| spellingShingle | Luigi Pistelli Clementina Sansone Arianna Smerilli Marco Festa Douglas M. Noonan Adriana Albini Christophe Brunet MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents Marine Drugs diatoxanthin alloxanthin oxidative stress carotenoids anti-inflammation ROS |
| title | MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents |
| title_full | MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents |
| title_fullStr | MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents |
| title_full_unstemmed | MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents |
| title_short | MMP-9 and IL-1β as Targets for Diatoxanthin and Related Microalgal Pigments: Potential Chemopreventive and Photoprotective Agents |
| title_sort | mmp 9 and il 1β as targets for diatoxanthin and related microalgal pigments potential chemopreventive and photoprotective agents |
| topic | diatoxanthin alloxanthin oxidative stress carotenoids anti-inflammation ROS |
| url | https://www.mdpi.com/1660-3397/19/7/354 |
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