The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus
The polymerase acidic (PA) protein is the third subunit of the influenza A virus polymerase. In recent years, studies have shown that PA plays an important role in overcoming the host species barrier and host adaptation of the avian influenza virus (AIV). The objective of this study was to elucidate...
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MDPI AG
2019-01-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/11/1/40 |
| _version_ | 1818536683243569152 |
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| author | Mengchan Hao Shaojie Han Dan Meng Rong Li Jing Lin Meng Wang Tong Zhou Tongjie Chai |
| author_facet | Mengchan Hao Shaojie Han Dan Meng Rong Li Jing Lin Meng Wang Tong Zhou Tongjie Chai |
| author_sort | Mengchan Hao |
| collection | DOAJ |
| description | The polymerase acidic (PA) protein is the third subunit of the influenza A virus polymerase. In recent years, studies have shown that PA plays an important role in overcoming the host species barrier and host adaptation of the avian influenza virus (AIV). The objective of this study was to elucidate the role of the PA subunit on the replication and airborne transmission of the H9N2 subtype AIV. By reverse genetics, a reassortant rSD01-PA was derived from the H9N2 subtype AIV A/Chicken/Shandong/01/2008 (SD01) by introducing the PA gene from the pandemic influenza A H1N1 virus A/swine/Shandong/07/2011 (SD07). Specific pathogen-free (SPF) chickens and guinea pigs were selected as the animal models for replication and aerosol transmission studies. Results show that rSD01-PA lost the ability of airborne transmission among SPF chickens because of the single substitution of the PA gene. However, rSD01-PA could infect guinea pigs through direct contact, while the parental strain SD01 could not, even though the infection of rSD01-PA could not be achieved through aerosol. In summary, our results indicate that the protein encoded by the PA gene plays a key role in replication and airborne transmission of the H9N2 subtype AIV. |
| first_indexed | 2024-12-11T18:41:06Z |
| format | Article |
| id | doaj.art-dcb3419da23148789b2c634b2c8feaff |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-11T18:41:06Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-dcb3419da23148789b2c634b2c8feaff2022-12-22T00:54:36ZengMDPI AGViruses1999-49152019-01-011114010.3390/v11010040v11010040The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza VirusMengchan Hao0Shaojie Han1Dan Meng2Rong Li3Jing Lin4Meng Wang5Tong Zhou6Tongjie Chai7College of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Taian 271018, ChinaThe polymerase acidic (PA) protein is the third subunit of the influenza A virus polymerase. In recent years, studies have shown that PA plays an important role in overcoming the host species barrier and host adaptation of the avian influenza virus (AIV). The objective of this study was to elucidate the role of the PA subunit on the replication and airborne transmission of the H9N2 subtype AIV. By reverse genetics, a reassortant rSD01-PA was derived from the H9N2 subtype AIV A/Chicken/Shandong/01/2008 (SD01) by introducing the PA gene from the pandemic influenza A H1N1 virus A/swine/Shandong/07/2011 (SD07). Specific pathogen-free (SPF) chickens and guinea pigs were selected as the animal models for replication and aerosol transmission studies. Results show that rSD01-PA lost the ability of airborne transmission among SPF chickens because of the single substitution of the PA gene. However, rSD01-PA could infect guinea pigs through direct contact, while the parental strain SD01 could not, even though the infection of rSD01-PA could not be achieved through aerosol. In summary, our results indicate that the protein encoded by the PA gene plays a key role in replication and airborne transmission of the H9N2 subtype AIV.http://www.mdpi.com/1999-4915/11/1/40H9N2 AIVpandemic 2009 H1N1 virusreassortmentreplicationairborne transmission |
| spellingShingle | Mengchan Hao Shaojie Han Dan Meng Rong Li Jing Lin Meng Wang Tong Zhou Tongjie Chai The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus Viruses H9N2 AIV pandemic 2009 H1N1 virus reassortment replication airborne transmission |
| title | The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus |
| title_full | The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus |
| title_fullStr | The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus |
| title_full_unstemmed | The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus |
| title_short | The PA Subunit of the Influenza Virus Polymerase Complex Affects Replication and Airborne Transmission of the H9N2 Subtype Avian Influenza Virus |
| title_sort | pa subunit of the influenza virus polymerase complex affects replication and airborne transmission of the h9n2 subtype avian influenza virus |
| topic | H9N2 AIV pandemic 2009 H1N1 virus reassortment replication airborne transmission |
| url | http://www.mdpi.com/1999-4915/11/1/40 |
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