Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent models
Summary: Intravital microscopy (IVM) involves surgical procedures to expose the internal organs of live anesthetized animals to visualize fluorescently labeled components in situ, in vivo at subcellular resolution. Here, we provide an IVM protocol for time-lapse imaging of dynamic Trypanosoma brucei...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003306 |
| _version_ | 1818470612750827520 |
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| author | Mariana De Niz Luisa M. Figueiredo |
| author_facet | Mariana De Niz Luisa M. Figueiredo |
| author_sort | Mariana De Niz |
| collection | DOAJ |
| description | Summary: Intravital microscopy (IVM) involves surgical procedures to expose the internal organs of live anesthetized animals to visualize fluorescently labeled components in situ, in vivo at subcellular resolution. Here, we provide an IVM protocol for time-lapse imaging of dynamic Trypanosoma brucei-host interactions in ten mammalian organs and in systemic circulation. We describe intraperitoneal or intradermal injection of mice with T. brucei. We then detail surgical procedures to prepare ten organs for IVM, followed by imaging of host-T. brucei interactions.For complete details on the use and execution of this protocol, please refer to De Niz et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T21:39:19Z |
| format | Article |
| id | doaj.art-dcb3682748eb4083b6575e63e0797f52 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T21:39:19Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-dcb3682748eb4083b6575e63e0797f522022-12-22T02:28:49ZengElsevierSTAR Protocols2666-16672022-06-0132101450Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent modelsMariana De Niz0Luisa M. Figueiredo1Instituto de Medicina Molecular - João lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal; Corresponding authorInstituto de Medicina Molecular - João lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, PortugalSummary: Intravital microscopy (IVM) involves surgical procedures to expose the internal organs of live anesthetized animals to visualize fluorescently labeled components in situ, in vivo at subcellular resolution. Here, we provide an IVM protocol for time-lapse imaging of dynamic Trypanosoma brucei-host interactions in ten mammalian organs and in systemic circulation. We describe intraperitoneal or intradermal injection of mice with T. brucei. We then detail surgical procedures to prepare ten organs for IVM, followed by imaging of host-T. brucei interactions.For complete details on the use and execution of this protocol, please refer to De Niz et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003306Cell BiologyMicrobiologyMicroscopyModel Organisms |
| spellingShingle | Mariana De Niz Luisa M. Figueiredo Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent models STAR Protocols Cell Biology Microbiology Microscopy Model Organisms |
| title | Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent models |
| title_full | Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent models |
| title_fullStr | Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent models |
| title_full_unstemmed | Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent models |
| title_short | Surgical and intravital microscopy protocol to image Trypanosoma brucei–host interactions in live rodent models |
| title_sort | surgical and intravital microscopy protocol to image trypanosoma brucei host interactions in live rodent models |
| topic | Cell Biology Microbiology Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722003306 |
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