Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 Software
Identification of microorganisms by MALDI-TOF MS has become a popular method in the past 20 years. Strain Solution ver. 2 software appended with MALDI-TOF MS enables accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. In...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/1/202 |
| _version_ | 1797438487992991744 |
|---|---|
| author | Teruyo Ojima-Kato Satomi Nagai Akane Fujita Junko Sakata Hiroto Tamura |
| author_facet | Teruyo Ojima-Kato Satomi Nagai Akane Fujita Junko Sakata Hiroto Tamura |
| author_sort | Teruyo Ojima-Kato |
| collection | DOAJ |
| description | Identification of microorganisms by MALDI-TOF MS has become a popular method in the past 20 years. Strain Solution ver. 2 software appended with MALDI-TOF MS enables accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. In this study, we constructed a theoretical mass database with the validated biomarkers to proteotype <i>Campylobacter jejuni</i>. Using 10 strains belonging to <i>Campylobacter</i> spp. available from culture collections and 41 <i>Campylobacter jejuni</i> strains isolated from humans and foods, the ribosomal protein subunits L36, L32, S14, L24, L23, L7/L12, and S11 could be selected as the effective biomarkers for the proteotyping of <i>C. jejuni</i> at MALDI-TOF MS. An accurate database of their theoretical mass-based values was constructed by matching these gene DNA sequences and the observed mass peaks. We attempted to automatically classify 41 strains isolated from nature using this database and Strain Solution ver. 2 software, and 38 strains (93%) were correctly classified into the intended group based on the theoretical mass-based values. Thus, the seven biomarkers found in this study and Strain Solution ver. 2 are promising for the proteotyping of <i>C. jejuni</i> by MALDI-TOF MS. |
| first_indexed | 2024-03-09T11:38:39Z |
| format | Article |
| id | doaj.art-dcb37cadcd504539a905d3b11b14ae87 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:38:39Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-dcb37cadcd504539a905d3b11b14ae872023-11-30T23:37:54ZengMDPI AGMicroorganisms2076-26072023-01-0111120210.3390/microorganisms11010202Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 SoftwareTeruyo Ojima-Kato0Satomi Nagai1Akane Fujita2Junko Sakata3Hiroto Tamura4School of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya 468-0073, JapanSchool of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya 468-0073, JapanSchool of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya 468-0073, JapanOsaka Institute of Public Health, Nakamichi, Higashinari-ku, Osaka 537-0025, JapanSchool of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya 468-0073, JapanIdentification of microorganisms by MALDI-TOF MS has become a popular method in the past 20 years. Strain Solution ver. 2 software appended with MALDI-TOF MS enables accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. In this study, we constructed a theoretical mass database with the validated biomarkers to proteotype <i>Campylobacter jejuni</i>. Using 10 strains belonging to <i>Campylobacter</i> spp. available from culture collections and 41 <i>Campylobacter jejuni</i> strains isolated from humans and foods, the ribosomal protein subunits L36, L32, S14, L24, L23, L7/L12, and S11 could be selected as the effective biomarkers for the proteotyping of <i>C. jejuni</i> at MALDI-TOF MS. An accurate database of their theoretical mass-based values was constructed by matching these gene DNA sequences and the observed mass peaks. We attempted to automatically classify 41 strains isolated from nature using this database and Strain Solution ver. 2 software, and 38 strains (93%) were correctly classified into the intended group based on the theoretical mass-based values. Thus, the seven biomarkers found in this study and Strain Solution ver. 2 are promising for the proteotyping of <i>C. jejuni</i> by MALDI-TOF MS.https://www.mdpi.com/2076-2607/11/1/202<i>Campylobacter jejuni</i>MALDI-TOF MSproteotypingstrain solutiontheoretical mass database |
| spellingShingle | Teruyo Ojima-Kato Satomi Nagai Akane Fujita Junko Sakata Hiroto Tamura Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 Software Microorganisms <i>Campylobacter jejuni</i> MALDI-TOF MS proteotyping strain solution theoretical mass database |
| title | Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 Software |
| title_full | Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 Software |
| title_fullStr | Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 Software |
| title_full_unstemmed | Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 Software |
| title_short | Proteotyping of <i>Campylobacter jejuni</i> by MALDI-TOF MS and Strain Solution Version 2 Software |
| title_sort | proteotyping of i campylobacter jejuni i by maldi tof ms and strain solution version 2 software |
| topic | <i>Campylobacter jejuni</i> MALDI-TOF MS proteotyping strain solution theoretical mass database |
| url | https://www.mdpi.com/2076-2607/11/1/202 |
| work_keys_str_mv | AT teruyoojimakato proteotypingoficampylobacterjejuniibymalditofmsandstrainsolutionversion2software AT satominagai proteotypingoficampylobacterjejuniibymalditofmsandstrainsolutionversion2software AT akanefujita proteotypingoficampylobacterjejuniibymalditofmsandstrainsolutionversion2software AT junkosakata proteotypingoficampylobacterjejuniibymalditofmsandstrainsolutionversion2software AT hirototamura proteotypingoficampylobacterjejuniibymalditofmsandstrainsolutionversion2software |