| Summary: | The indoor air quality of healthcare and care facilities is poorly studied. The aim of this study was to qualitatively and quantitatively describe the chemical pollution and the microbiological contaminations of the indoor environment of these facilities. Methods: A wide range of chemical compounds (39 volatile and 13 semi-volatile organic compounds, carbon dioxide, fine particulate matter) and microorganisms (fungi and bacteria) were studied. Sampling campaigns were conducted in two French cities in summer 2018 and winter 2019 in six private healthcare facilities (general practitioner’s offices, dental offices, pharmacies) and four care facilities (nursing homes). Results: The highest median concentrations of chemical compounds (μg/m<sup>3</sup>) were measured for alcohols (ethanol: 378.9 and isopropanol: 23.6), ketones (acetone: 18.8), aldehydes (formaldehyde: 11.4 and acetaldehyde: 6.5) and terpenes (limonene: 4.3). The median concentration of PM<sub>2.5</sub> was 9.0 µg/m<sup>3</sup>. The main bacteria of these indoor environments were <i>Staphylococcus</i>, <i>Micrococcus</i> and <i>Bacillus</i> genera, with median bacterial concentrations in the indoor air of 14 cfu/m<sup>3</sup>. The two major fungal genera were <i>Cladosporium</i> and <i>Penicillium</i>, with median fungal concentrations of 7 cfu/m<sup>3</sup>. Conclusions: Indoor air in healthcare and care facilities contains a complex mixture of many pollutants found in higher concentrations compared to the indoor air in French hospitals in a previous study.
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