Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue
Abstract Objective Breast cancer is one of the most common health problems. It has been suggested that several risk factors, either considered as external or internal, play a critical role in the pathogenesis of breast cancer, which among them, HERV-k, has the most fundamental role. In the present s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Infectious Agents and Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13027-019-0260-7 |
| _version_ | 1818331368730394624 |
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| author | Shaian Tavakolian Hossein Goudarzi Ebrahim Faghihloo |
| author_facet | Shaian Tavakolian Hossein Goudarzi Ebrahim Faghihloo |
| author_sort | Shaian Tavakolian |
| collection | DOAJ |
| description | Abstract Objective Breast cancer is one of the most common health problems. It has been suggested that several risk factors, either considered as external or internal, play a critical role in the pathogenesis of breast cancer, which among them, HERV-k, has the most fundamental role. In the present study, we aimed to evaluate the role of HERV-k env, gag, rec, np9 expressions in breast cancer progression. Materials and methods We collected 40 breast cancer tissues and their normal adjacent ones. After extracting the RNA of breast samples, we evaluated the expression of HERV-k env, gag, rec, np9 by using Quantitative real-time PCR (qRT-PCR). Results The resulting data revealed that while there was a meaningful increase in the expression level of HERV-k env, gag and np9 in breast cancer tissues (P ≤ 0.01, 0.05, 0.05, respectively), we failed to find any significant elevation in the expression level of rec mRNA level. Conclusion The results of our study suggested that there is a plausible correlation between the mRNA expression level of HERV-K env, gag and np9 and the progression of breast cancer, proposing these markers as promising biomarkers to diagnose breast cancer. |
| first_indexed | 2024-12-13T13:18:45Z |
| format | Article |
| id | doaj.art-dcb61728e46547a1947ea02700a19655 |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-12-13T13:18:45Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-dcb61728e46547a1947ea02700a196552022-12-21T23:44:28ZengBMCInfectious Agents and Cancer1750-93782019-11-011411510.1186/s13027-019-0260-7Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissueShaian Tavakolian0Hossein Goudarzi1Ebrahim Faghihloo2Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesAbstract Objective Breast cancer is one of the most common health problems. It has been suggested that several risk factors, either considered as external or internal, play a critical role in the pathogenesis of breast cancer, which among them, HERV-k, has the most fundamental role. In the present study, we aimed to evaluate the role of HERV-k env, gag, rec, np9 expressions in breast cancer progression. Materials and methods We collected 40 breast cancer tissues and their normal adjacent ones. After extracting the RNA of breast samples, we evaluated the expression of HERV-k env, gag, rec, np9 by using Quantitative real-time PCR (qRT-PCR). Results The resulting data revealed that while there was a meaningful increase in the expression level of HERV-k env, gag and np9 in breast cancer tissues (P ≤ 0.01, 0.05, 0.05, respectively), we failed to find any significant elevation in the expression level of rec mRNA level. Conclusion The results of our study suggested that there is a plausible correlation between the mRNA expression level of HERV-K env, gag and np9 and the progression of breast cancer, proposing these markers as promising biomarkers to diagnose breast cancer.https://doi.org/10.1186/s13027-019-0260-7Envnp9RecGagBreast cancer |
| spellingShingle | Shaian Tavakolian Hossein Goudarzi Ebrahim Faghihloo Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue Infectious Agents and Cancer Env np9 Rec Gag Breast cancer |
| title | Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue |
| title_full | Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue |
| title_fullStr | Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue |
| title_full_unstemmed | Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue |
| title_short | Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue |
| title_sort | evaluating the expression level of herv k env np9 rec and gag in breast tissue |
| topic | Env np9 Rec Gag Breast cancer |
| url | https://doi.org/10.1186/s13027-019-0260-7 |
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