Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis Biofilm
Introduction: Considering the clinical importance of microbial biofilms in endodontic infections and the role of the different irrigating solutions in their destruction, the present study aimed to investigate the efficacy of Peganum Harmala extract and Sodium Hypochlorite on the destruction of Ent...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2014-08-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_id=2749&sid=1&slc_lang=en |
| _version_ | 1818807881392193536 |
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| author | M Tabrizizadeh H Zandi MH Mosaddegh Mehrjardi H Mahmodizadeh |
| author_facet | M Tabrizizadeh H Zandi MH Mosaddegh Mehrjardi H Mahmodizadeh |
| author_sort | M Tabrizizadeh |
| collection | DOAJ |
| description | Introduction: Considering the clinical importance of microbial biofilms in endodontic infections and the role of the different irrigating solutions in their destruction, the present study aimed to investigate the efficacy of Peganum Harmala extract and Sodium Hypochlorite on the destruction of Enterococcus faeclis biofilm.
Methods: For conducting this lab trial, root canals of 45 extracted teeth were prepared and E. faecalis biofilms were formed within the root canals. Then the teeth were randomly divided into three groups of 15. Samples were irrigated with Normal Saline in group 1, to serve as controls. 5/25% sodium hypochlorite was applied in group 2 and Peganum Harmala extract was utilized in group 3. All of the roots were irrigated by 15 cc of irrigation solutions for 15 min. Dentin chips were collected from the root canal walls and were weighed as well. Then the chips were used to prepare a suspension. The colony-forming unit (CFU) counting technique was used to determine remaining bacterial counts. The data were analyzed via Mann-whitney and kruskal-wallis tests.
Results: A significant reduction of CFU was observed in the Peganum Harmala extract and sodium hypochlorite groups compared to the control group(p<0/05). No significant difference was reported between Peganum Harmala extract and sodium hypochlorite groups (p>0.05).
Conclusion: The antibacterial activity of Peganum Harmala extract is found to be comparable with that of 5/25% sodium hypochlorite on the Enterococcus faeclis biofilm. |
| first_indexed | 2024-12-18T19:32:42Z |
| format | Article |
| id | doaj.art-dcb762cab0db47beaaa023845463e4d7 |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-18T19:32:42Z |
| publishDate | 2014-08-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-dcb762cab0db47beaaa023845463e4d72022-12-21T20:55:41ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332014-08-0122312561264Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis BiofilmM Tabrizizadeh0H Zandi 1MH Mosaddegh Mehrjardi 2H Mahmodizadeh3Department of Endodontics, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Bacteriology, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Pharmacology, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Endodontics, Shahid Sadoughi University of Medical Sciences, Yazd, IranIntroduction: Considering the clinical importance of microbial biofilms in endodontic infections and the role of the different irrigating solutions in their destruction, the present study aimed to investigate the efficacy of Peganum Harmala extract and Sodium Hypochlorite on the destruction of Enterococcus faeclis biofilm. Methods: For conducting this lab trial, root canals of 45 extracted teeth were prepared and E. faecalis biofilms were formed within the root canals. Then the teeth were randomly divided into three groups of 15. Samples were irrigated with Normal Saline in group 1, to serve as controls. 5/25% sodium hypochlorite was applied in group 2 and Peganum Harmala extract was utilized in group 3. All of the roots were irrigated by 15 cc of irrigation solutions for 15 min. Dentin chips were collected from the root canal walls and were weighed as well. Then the chips were used to prepare a suspension. The colony-forming unit (CFU) counting technique was used to determine remaining bacterial counts. The data were analyzed via Mann-whitney and kruskal-wallis tests. Results: A significant reduction of CFU was observed in the Peganum Harmala extract and sodium hypochlorite groups compared to the control group(p<0/05). No significant difference was reported between Peganum Harmala extract and sodium hypochlorite groups (p>0.05). Conclusion: The antibacterial activity of Peganum Harmala extract is found to be comparable with that of 5/25% sodium hypochlorite on the Enterococcus faeclis biofilm.http://jssu.ssu.ac.ir/browse.php?a_id=2749&sid=1&slc_lang=enAntimicrobial; Enterococcus Faeclis; Peganum Harmala Extract; Sodium Hypochlorite |
| spellingShingle | M Tabrizizadeh H Zandi MH Mosaddegh Mehrjardi H Mahmodizadeh Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis Biofilm Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Antimicrobial; Enterococcus Faeclis; Peganum Harmala Extract; Sodium Hypochlorite |
| title | Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis Biofilm |
| title_full | Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis Biofilm |
| title_fullStr | Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis Biofilm |
| title_full_unstemmed | Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis Biofilm |
| title_short | Comparing the Antibacterial Effect of Peganum Harmala Extract and 5/25% Sodium Hypochlorite on Enterococcus Faeclis Biofilm |
| title_sort | comparing the antibacterial effect of peganum harmala extract and 5 25 sodium hypochlorite on enterococcus faeclis biofilm |
| topic | Antimicrobial; Enterococcus Faeclis; Peganum Harmala Extract; Sodium Hypochlorite |
| url | http://jssu.ssu.ac.ir/browse.php?a_id=2749&sid=1&slc_lang=en |
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