Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants
Silene latifolia and S. dioica are model systems in studies of plant reproduction, chromosome evolution and sexual dimorphism, but sexing of plants based on morphology is only possible from flowering stage onwards. Both species show homogametic females (XX) and heterogametic males (XY). • Here we de...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016124001626 |
| _version_ | 1797203060128219136 |
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| author | Anna M. Hewett Julia Sánchez Vilas Frank Hailer |
| author_facet | Anna M. Hewett Julia Sánchez Vilas Frank Hailer |
| author_sort | Anna M. Hewett |
| collection | DOAJ |
| description | Silene latifolia and S. dioica are model systems in studies of plant reproduction, chromosome evolution and sexual dimorphism, but sexing of plants based on morphology is only possible from flowering stage onwards. Both species show homogametic females (XX) and heterogametic males (XY). • Here we developed two assays (primer pairs ss816 and ss441) for molecular sexing of S. latifolia and S. dioica, targeting length polymorphisms between the X and Y-linked copies of the spermidine synthase gene.The two assays were successful in identifying known (flowering-stage) males and females from UK and Spanish populations, with an error rate of 3.1% (ss816; successful for both species) and 0% (ss441, only successful for S. latifolia). Our assays therefore represent novel tools for rapid, robust and simple determination of the genotypic sex of S. latifolia and S. dioica. |
| first_indexed | 2024-04-24T08:13:19Z |
| format | Article |
| id | doaj.art-dcbde6bd0dc54c4d804fe76e90d50fd8 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-04-24T08:13:19Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-dcbde6bd0dc54c4d804fe76e90d50fd82024-04-17T04:49:16ZengElsevierMethodsX2215-01612024-06-0112102708Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plantsAnna M. Hewett0Julia Sánchez Vilas1Frank Hailer2School of Biosciences, Sir Martin Evans Building, Cardiff University, Cardiff CF10 3AX, UK; Department of Ecology and Evolution, University of Lausanne (UNIL), Lausanne 1015, SwitzerlandSchool of Biosciences, Sir Martin Evans Building, Cardiff University, Cardiff CF10 3AX, UK; Departamento de Bioloxía Funcional (Área de Ecoloxía) Facultade de Bioloxía, Universidade de Santiago de Compostelac/ Lope Gómez de Marzoa, s/n15782 Santiago de Compostela, Spain; Corresponding author at: School of Biosciences, Sir Martin Evans Building, Cardiff University, Cardiff CF10 3AX, UK.School of Biosciences, Sir Martin Evans Building, Cardiff University, Cardiff CF10 3AX, UK; Cardiff University-Institute of Zoology Joint Laboratory for Biocomplexity Research, Beijing, ChinaSilene latifolia and S. dioica are model systems in studies of plant reproduction, chromosome evolution and sexual dimorphism, but sexing of plants based on morphology is only possible from flowering stage onwards. Both species show homogametic females (XX) and heterogametic males (XY). • Here we developed two assays (primer pairs ss816 and ss441) for molecular sexing of S. latifolia and S. dioica, targeting length polymorphisms between the X and Y-linked copies of the spermidine synthase gene.The two assays were successful in identifying known (flowering-stage) males and females from UK and Spanish populations, with an error rate of 3.1% (ss816; successful for both species) and 0% (ss441, only successful for S. latifolia). Our assays therefore represent novel tools for rapid, robust and simple determination of the genotypic sex of S. latifolia and S. dioica.http://www.sciencedirect.com/science/article/pii/S2215016124001626PCR-based assays for sexing Silene plants |
| spellingShingle | Anna M. Hewett Julia Sánchez Vilas Frank Hailer Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants MethodsX PCR-based assays for sexing Silene plants |
| title | Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants |
| title_full | Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants |
| title_fullStr | Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants |
| title_full_unstemmed | Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants |
| title_short | Two novel PCR-based assays for sexing of Silene latifolia and Silene dioica plants |
| title_sort | two novel pcr based assays for sexing of silene latifolia and silene dioica plants |
| topic | PCR-based assays for sexing Silene plants |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124001626 |
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