Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination
Genetic recombination in RNA viruses is an important evolutionary mechanism. It contributes to population diversity, host/tissue adaptation, and compromises vaccine efficacy. Both the molecular mechanism and initial products of recombination are relatively poorly understood. We used an established p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/5/916 |
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| author | Fadi G. Alnaji Kirsten Bentley Ashley Pearson Andrew Woodman Jonathan Moore Helen Fox Andrew J. Macadam David J. Evans |
| author_facet | Fadi G. Alnaji Kirsten Bentley Ashley Pearson Andrew Woodman Jonathan Moore Helen Fox Andrew J. Macadam David J. Evans |
| author_sort | Fadi G. Alnaji |
| collection | DOAJ |
| description | Genetic recombination in RNA viruses is an important evolutionary mechanism. It contributes to population diversity, host/tissue adaptation, and compromises vaccine efficacy. Both the molecular mechanism and initial products of recombination are relatively poorly understood. We used an established poliovirus-based in vitro recombination assay to investigate the roles of sequence identity and RNA structure, implicated or inferred from an analysis of circulating recombinant viruses, in the process. In addition, we used next-generation sequencing to investigate the early products of recombination after cellular coinfection with different poliovirus serotypes. In independent studies, we find no evidence for a role for RNA identity or structure in determining recombination junctions location. Instead, genome function and fitness are of greater importance in determining the identity of recombinant progeny. These studies provide further insights into this important evolutionary mechanism and emphasize the critical nature of the selection process on a mixed virus population. |
| first_indexed | 2024-03-10T01:37:52Z |
| format | Article |
| id | doaj.art-dcbf46d13af245678448a7d5fbacdc79 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T01:37:52Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-dcbf46d13af245678448a7d5fbacdc792023-11-23T13:30:12ZengMDPI AGViruses1999-49152022-04-0114591610.3390/v14050916Generated Randomly and Selected Functionally? The Nature of Enterovirus RecombinationFadi G. Alnaji0Kirsten Bentley1Ashley Pearson2Andrew Woodman3Jonathan Moore4Helen Fox5Andrew J. Macadam6David J. Evans7Department of Microbiology, University of Illinois Champaign-Urbana, 601 E. John St, Urbana, IL 61801, USADivision Infection & Immunity, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UKBiomedical Sciences Research Complex and School of Biology, University of St Andrew, St. Andrews KY16 9ST, UKDepartment of Biochemistry & Molecular Biology, 201 Althouse Laboratory, University Park, PA 16802, USATropic Biosciences, Bioinformatics Group, Norwich NR4 7JG, UKDivision of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UKDivision of Virology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UKBiomedical Sciences Research Complex and School of Biology, University of St Andrew, St. Andrews KY16 9ST, UKGenetic recombination in RNA viruses is an important evolutionary mechanism. It contributes to population diversity, host/tissue adaptation, and compromises vaccine efficacy. Both the molecular mechanism and initial products of recombination are relatively poorly understood. We used an established poliovirus-based in vitro recombination assay to investigate the roles of sequence identity and RNA structure, implicated or inferred from an analysis of circulating recombinant viruses, in the process. In addition, we used next-generation sequencing to investigate the early products of recombination after cellular coinfection with different poliovirus serotypes. In independent studies, we find no evidence for a role for RNA identity or structure in determining recombination junctions location. Instead, genome function and fitness are of greater importance in determining the identity of recombinant progeny. These studies provide further insights into this important evolutionary mechanism and emphasize the critical nature of the selection process on a mixed virus population.https://www.mdpi.com/1999-4915/14/5/916positive-sense RNA virusesviral evolutionrecombinationnext-generation sequencing |
| spellingShingle | Fadi G. Alnaji Kirsten Bentley Ashley Pearson Andrew Woodman Jonathan Moore Helen Fox Andrew J. Macadam David J. Evans Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination Viruses positive-sense RNA viruses viral evolution recombination next-generation sequencing |
| title | Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination |
| title_full | Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination |
| title_fullStr | Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination |
| title_full_unstemmed | Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination |
| title_short | Generated Randomly and Selected Functionally? The Nature of Enterovirus Recombination |
| title_sort | generated randomly and selected functionally the nature of enterovirus recombination |
| topic | positive-sense RNA viruses viral evolution recombination next-generation sequencing |
| url | https://www.mdpi.com/1999-4915/14/5/916 |
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