Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/15/9/293 |
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| author | Faten Ben Amor Fatma Elleuch Hajer Ben Hlima Matthieu Garnier Bruno Saint-Jean Mohamed Barkallah Chantal Pichon Slim Abdelkafi Imen Fendri |
| author_facet | Faten Ben Amor Fatma Elleuch Hajer Ben Hlima Matthieu Garnier Bruno Saint-Jean Mohamed Barkallah Chantal Pichon Slim Abdelkafi Imen Fendri |
| author_sort | Faten Ben Amor |
| collection | DOAJ |
| description | The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production. |
| first_indexed | 2024-04-14T04:27:49Z |
| format | Article |
| id | doaj.art-dcc086a200f7435da9f87d5a906d32ad |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T04:27:49Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-dcc086a200f7435da9f87d5a906d32ad2022-12-22T02:12:15ZengMDPI AGMarine Drugs1660-33972017-09-0115929310.3390/md15090293md15090293Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid ProductionFaten Ben Amor0Fatma Elleuch1Hajer Ben Hlima2Matthieu Garnier3Bruno Saint-Jean4Mohamed Barkallah5Chantal Pichon6Slim Abdelkafi7Imen Fendri8Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3038, TunisiaLaboratoire de Biotechnologie Végétale Appliquée à l’Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax 3000, TunisiaUnité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3038, TunisiaLaboratoire BRM-PBA Ifremer, Nantes 44311, FranceLaboratoire BRM-PBA Ifremer, Nantes 44311, FranceUnité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3038, TunisiaCenter for Molecular Biophysics (CBM), CNRS UPR 4301, Orléans 45071, FranceUnité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3038, TunisiaLaboratoire de Biotechnologie Végétale Appliquée à l’Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax 3000, TunisiaThe green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel electrophoresis, was performed from standard and optimized cultures. We detected 127 protein spots which were significantly differentially expressed between standard and optimized cultures. Among them 16 protein spots were identified with mass spectrometry and grouped into different functional categories using KEGG (Kyoto Encyclopedia of Genes and Genomes) such as photosynthetic Calvin cycle, regulation/defense, energy metabolism, glycolysis, and cellular processes. The current study could be of great interest in providing information on the effect of stressful conditions in microalgae carotenoid production.https://www.mdpi.com/1660-3397/15/9/293proteomeDunaliella18S rRNA genecarotenoidsbidimensional gel electrophoresismass spectrometry |
| spellingShingle | Faten Ben Amor Fatma Elleuch Hajer Ben Hlima Matthieu Garnier Bruno Saint-Jean Mohamed Barkallah Chantal Pichon Slim Abdelkafi Imen Fendri Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production Marine Drugs proteome Dunaliella 18S rRNA gene carotenoids bidimensional gel electrophoresis mass spectrometry |
| title | Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production |
| title_full | Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production |
| title_fullStr | Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production |
| title_full_unstemmed | Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production |
| title_short | Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production |
| title_sort | proteomic analysis of the chlorophyta dunaliella new strain al 1 revealed global changes of metabolism during high carotenoid production |
| topic | proteome Dunaliella 18S rRNA gene carotenoids bidimensional gel electrophoresis mass spectrometry |
| url | https://www.mdpi.com/1660-3397/15/9/293 |
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