Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil
Five novel strains of Photobacterium (A-394T, A-373, A-379, A-397 and A-398) were isolated from bleached coral Madracis decactis (scleractinian) in the remote St Peter & St Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. Healthy M. decactis specimens were also surveyed, but no strains were rela...
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| Format: | Article |
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PeerJ Inc.
2014-06-01
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| Online Access: | https://peerj.com/articles/427.pdf |
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| author | Ana Paula B. Moreira Gwen Duytschaever Luciane A. Chimetto Tonon Adriana M. Fróes Louisi S. de Oliveira Gilberto M. Amado-Filho Ronaldo B. Francini-Filho Paul De Vos Jean Swings Cristiane C. Thompson Fabiano L. Thompson |
| author_facet | Ana Paula B. Moreira Gwen Duytschaever Luciane A. Chimetto Tonon Adriana M. Fróes Louisi S. de Oliveira Gilberto M. Amado-Filho Ronaldo B. Francini-Filho Paul De Vos Jean Swings Cristiane C. Thompson Fabiano L. Thompson |
| author_sort | Ana Paula B. Moreira |
| collection | DOAJ |
| description | Five novel strains of Photobacterium (A-394T, A-373, A-379, A-397 and A-398) were isolated from bleached coral Madracis decactis (scleractinian) in the remote St Peter & St Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. Healthy M. decactis specimens were also surveyed, but no strains were related to them. The novel isolates formed a distinct lineage based on the 16S rRNA, recA, and rpoA gene sequences analysis. Their closest phylogenetic neighbours were Photobacterium rosenbergii, P. gaetbulicola, and P. lutimaris, sharing 96.6 to 95.8% 16S rRNA gene sequence similarity. The novel species can be differentiated from the closest neighbours by several phenotypic and chemotaxonomic markers. It grows at pH 11, produces tryptophane deaminase, presents the fatty acid C18:0, but lacks C16:0 iso. The whole cell protein profile, based in MALDI-TOF MS, distinguished the strains of the novel species among each other and from the closest neighbors. In addition, we are releasing the whole genome sequence of the type strain. The name Photobacterium sanctipauli sp. nov. is proposed for this taxon. The G + C content of the type strain A-394T (= LMG27910T = CAIM1892T) is 48.2 mol%. |
| first_indexed | 2024-03-09T07:06:43Z |
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| language | English |
| last_indexed | 2024-03-09T07:06:43Z |
| publishDate | 2014-06-01 |
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| spelling | doaj.art-dcc580ff1dc54363853dea7da51b41dc2023-12-03T09:29:59ZengPeerJ Inc.PeerJ2167-83592014-06-012e42710.7717/peerj.427427Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, BrazilAna Paula B. Moreira0Gwen Duytschaever1Luciane A. Chimetto Tonon2Adriana M. Fróes3Louisi S. de Oliveira4Gilberto M. Amado-Filho5Ronaldo B. Francini-Filho6Paul De Vos7Jean Swings8Cristiane C. Thompson9Fabiano L. Thompson10Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstitute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstitute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstitute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstitute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilBotanical Garden Research Institute (JBRJ), Rio de Janeiro, BrazilDepartment of Environment and Engineering, Federal University of Paraíba (UFPB), BrazilBCCM/LMG Bacteria Collection, Ghent University, Ghent, BelgiumBCCM/LMG Bacteria Collection, Ghent University, Ghent, BelgiumInstitute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstitute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilFive novel strains of Photobacterium (A-394T, A-373, A-379, A-397 and A-398) were isolated from bleached coral Madracis decactis (scleractinian) in the remote St Peter & St Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. Healthy M. decactis specimens were also surveyed, but no strains were related to them. The novel isolates formed a distinct lineage based on the 16S rRNA, recA, and rpoA gene sequences analysis. Their closest phylogenetic neighbours were Photobacterium rosenbergii, P. gaetbulicola, and P. lutimaris, sharing 96.6 to 95.8% 16S rRNA gene sequence similarity. The novel species can be differentiated from the closest neighbours by several phenotypic and chemotaxonomic markers. It grows at pH 11, produces tryptophane deaminase, presents the fatty acid C18:0, but lacks C16:0 iso. The whole cell protein profile, based in MALDI-TOF MS, distinguished the strains of the novel species among each other and from the closest neighbors. In addition, we are releasing the whole genome sequence of the type strain. The name Photobacterium sanctipauli sp. nov. is proposed for this taxon. The G + C content of the type strain A-394T (= LMG27910T = CAIM1892T) is 48.2 mol%.https://peerj.com/articles/427.pdf Photobacterium sanctipauli St Paul’s rocksCoral bleachingNew speciesGenomic taxonomy |
| spellingShingle | Ana Paula B. Moreira Gwen Duytschaever Luciane A. Chimetto Tonon Adriana M. Fróes Louisi S. de Oliveira Gilberto M. Amado-Filho Ronaldo B. Francini-Filho Paul De Vos Jean Swings Cristiane C. Thompson Fabiano L. Thompson Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil PeerJ Photobacterium sanctipauli St Paul’s rocks Coral bleaching New species Genomic taxonomy |
| title | Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
| title_full | Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
| title_fullStr | Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
| title_full_unstemmed | Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
| title_short | Photobacterium sanctipauli sp. nov. isolated from bleached Madracis decactis (Scleractinia) in the St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
| title_sort | photobacterium sanctipauli sp nov isolated from bleached madracis decactis scleractinia in the st peter st paul archipelago mid atlantic ridge brazil |
| topic | Photobacterium sanctipauli St Paul’s rocks Coral bleaching New species Genomic taxonomy |
| url | https://peerj.com/articles/427.pdf |
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