An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonists
Abstract Background Natural products constitute a promising class of therapeutics for the treatment of gingivitis and periodontitis as well as the maintenance of oral health. However, the limited understanding behind their potential mechanisms and modes of action have hampered their incorporation in...
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-020-2850-8 |
| _version_ | 1818597656196284416 |
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| author | Ana M. Chang Shatha Bamashmous Richard P. Darveau Sunethra Rajapakse |
| author_facet | Ana M. Chang Shatha Bamashmous Richard P. Darveau Sunethra Rajapakse |
| author_sort | Ana M. Chang |
| collection | DOAJ |
| description | Abstract Background Natural products constitute a promising class of therapeutics for the treatment of gingivitis and periodontitis as well as the maintenance of oral health. However, the limited understanding behind their potential mechanisms and modes of action have hampered their incorporation into popular western therapeutics. This in vitro study characterizes an Ayurvedic herbal extract mixture, which has been clinically shown to promote gingival health and homeostasis. Methods Telomerase immortalized gingival keratinocytes (TIGK) were infected with either Fusobacterium nucleatum cell wall, live F. nucleatum, IL-1β or TNF-α for 4 hours with and without the herbal extract. The immunomodulatory effects of the extract on host IL-8 production was measured by ELISA. Results It was found that the Ayurvedic herbal extract mixture inhibited gingival epithelial cell IL-8 expression in response to both bacterial and host cytokine agonists. The herbal extract inhibited IL-8 stimulated by F. nucleatum cell wall, live F. nucleatum, IL-1β, and TNF-α in a dose-dependent manner that was not a result of host cell death. Furthermore, the extract showed significantly different ID50 doses demonstrating the differential ability to modulate both stimulated and basal IL-8 levels. Conclusions In vitro investigation of this herbal extract mixture revealed that it has the ability to modulate gingival epithelial cell IL-8 expression in response to stimulation by bacterial components and host pro-inflammatory signals. This data demonstrates that the reduction in the gingival epithelial cell IL-8 response may in part be responsible for the previously reported ability of the Ayurvedic herbal extract mixture to reduce gingivitis in two separate human clinical studies. |
| first_indexed | 2024-12-16T11:51:16Z |
| format | Article |
| id | doaj.art-dcc9033871ec4f3c84b960b782cf277a |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-16T11:51:16Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-dcc9033871ec4f3c84b960b782cf277a2022-12-21T22:32:42ZengBMCBMC Complementary Medicine and Therapies2662-76712020-02-012011710.1186/s12906-020-2850-8An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonistsAna M. Chang0Shatha Bamashmous1Richard P. Darveau2Sunethra Rajapakse3Department of Oral Health Sciences, University of Washington School of DentistryDepartment of Oral Health Sciences, University of Washington School of DentistryDepartment of Periodontics, University of Washington School of DentistryDepartment of Oral Medicine and Periodontology, University of PeradeniyaAbstract Background Natural products constitute a promising class of therapeutics for the treatment of gingivitis and periodontitis as well as the maintenance of oral health. However, the limited understanding behind their potential mechanisms and modes of action have hampered their incorporation into popular western therapeutics. This in vitro study characterizes an Ayurvedic herbal extract mixture, which has been clinically shown to promote gingival health and homeostasis. Methods Telomerase immortalized gingival keratinocytes (TIGK) were infected with either Fusobacterium nucleatum cell wall, live F. nucleatum, IL-1β or TNF-α for 4 hours with and without the herbal extract. The immunomodulatory effects of the extract on host IL-8 production was measured by ELISA. Results It was found that the Ayurvedic herbal extract mixture inhibited gingival epithelial cell IL-8 expression in response to both bacterial and host cytokine agonists. The herbal extract inhibited IL-8 stimulated by F. nucleatum cell wall, live F. nucleatum, IL-1β, and TNF-α in a dose-dependent manner that was not a result of host cell death. Furthermore, the extract showed significantly different ID50 doses demonstrating the differential ability to modulate both stimulated and basal IL-8 levels. Conclusions In vitro investigation of this herbal extract mixture revealed that it has the ability to modulate gingival epithelial cell IL-8 expression in response to stimulation by bacterial components and host pro-inflammatory signals. This data demonstrates that the reduction in the gingival epithelial cell IL-8 response may in part be responsible for the previously reported ability of the Ayurvedic herbal extract mixture to reduce gingivitis in two separate human clinical studies.http://link.springer.com/article/10.1186/s12906-020-2850-8HerbalInterleukin-8PeriodontitisOral healthFusobacterium nucleatumInflammation |
| spellingShingle | Ana M. Chang Shatha Bamashmous Richard P. Darveau Sunethra Rajapakse An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonists BMC Complementary Medicine and Therapies Herbal Interleukin-8 Periodontitis Oral health Fusobacterium nucleatum Inflammation |
| title | An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonists |
| title_full | An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonists |
| title_fullStr | An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonists |
| title_full_unstemmed | An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonists |
| title_short | An Ayurvedic herbal extract inhibits oral epithelial cell IL-8 responses to host and bacterial agonists |
| title_sort | ayurvedic herbal extract inhibits oral epithelial cell il 8 responses to host and bacterial agonists |
| topic | Herbal Interleukin-8 Periodontitis Oral health Fusobacterium nucleatum Inflammation |
| url | http://link.springer.com/article/10.1186/s12906-020-2850-8 |
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