Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different Vitamins
There is a very vital antioxidant extracted from microgreen alga. <i>Chlorella vulgaris</i> has major advantages and requires high yield worldwide. Some microalgae require vitamins for their growth promotion. This study was held to determine the impact of different vitamins including Thi...
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| author | Ragaa A. Hamouda Amera Abd El Latif Ebtihal M. Elkaw Amenah S. Alotaibi Asma Massad Alenzi Hanafy A. Hamza |
| author_facet | Ragaa A. Hamouda Amera Abd El Latif Ebtihal M. Elkaw Amenah S. Alotaibi Asma Massad Alenzi Hanafy A. Hamza |
| author_sort | Ragaa A. Hamouda |
| collection | DOAJ |
| description | There is a very vital antioxidant extracted from microgreen alga. <i>Chlorella vulgaris</i> has major advantages and requires high yield worldwide. Some microalgae require vitamins for their growth promotion. This study was held to determine the impact of different vitamins including Thiamine (B1), Riboflavin (B2), Pyridoxine (B6), and Ascorbic acid (c) at concentrations of 0.02, 0.04, 0.06, and 0.08 mg/L of each. Each vitamin was added to the BG11 growth medium to determine the effect on growth, total carbohydrate, total protein, pigments content, antioxidant activities of <i>Chlorella vulgaris</i>. Moreover, antitumor effects of methanol extract of <i>C. vulgaris</i> without and with the supplement of thiamine against Human prostate cancer (PC-3), Hepatocellular carcinoma (HEPG-2), Colorectal carcinoma (HCT-116) and Epitheliod Carcinoma (Hela) was estimated in vitro. <i>C. vulgaris</i> supplemented with various vitamins showed a significant increase in biomass, pigment content, total protein, and total carbohydrates in comparison to the control. Thiamine was the best vitamin influencing as an antioxidant. <i>C. vulgaris</i> supplemented with thiamine had high antitumor effects in vitro. So, it’s necessary to add vitamins to BG11 media for enhancement of the growth and metabolites. |
| first_indexed | 2024-03-09T20:29:35Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T20:29:35Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dcc99f373c154b8291e7a65e2e62a00f2023-11-23T23:26:56ZengMDPI AGMolecules1420-30492022-02-01275160210.3390/molecules27051602Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different VitaminsRagaa A. Hamouda0Amera Abd El Latif1Ebtihal M. Elkaw2Amenah S. Alotaibi3Asma Massad Alenzi4Hanafy A. Hamza5Department of Biology, College of Sciences and Arts, Khulis, University of Jeddah, Jeddah 21959, Saudi ArabiaDepartment of Pharmacology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, EgyptDepartment of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat 32897, EgyptBiology Department, College of Sciences, Tabuk University, Tabuk 71491, Saudi ArabiaBiology Department, College of Sciences, Tabuk University, Tabuk 71491, Saudi ArabiaDepartment of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat 32897, EgyptThere is a very vital antioxidant extracted from microgreen alga. <i>Chlorella vulgaris</i> has major advantages and requires high yield worldwide. Some microalgae require vitamins for their growth promotion. This study was held to determine the impact of different vitamins including Thiamine (B1), Riboflavin (B2), Pyridoxine (B6), and Ascorbic acid (c) at concentrations of 0.02, 0.04, 0.06, and 0.08 mg/L of each. Each vitamin was added to the BG11 growth medium to determine the effect on growth, total carbohydrate, total protein, pigments content, antioxidant activities of <i>Chlorella vulgaris</i>. Moreover, antitumor effects of methanol extract of <i>C. vulgaris</i> without and with the supplement of thiamine against Human prostate cancer (PC-3), Hepatocellular carcinoma (HEPG-2), Colorectal carcinoma (HCT-116) and Epitheliod Carcinoma (Hela) was estimated in vitro. <i>C. vulgaris</i> supplemented with various vitamins showed a significant increase in biomass, pigment content, total protein, and total carbohydrates in comparison to the control. Thiamine was the best vitamin influencing as an antioxidant. <i>C. vulgaris</i> supplemented with thiamine had high antitumor effects in vitro. So, it’s necessary to add vitamins to BG11 media for enhancement of the growth and metabolites.https://www.mdpi.com/1420-3049/27/5/1602vitamins<i>Chlorella vulgaris</i>carbohydrateantioxidantantitumor |
| spellingShingle | Ragaa A. Hamouda Amera Abd El Latif Ebtihal M. Elkaw Amenah S. Alotaibi Asma Massad Alenzi Hanafy A. Hamza Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different Vitamins Molecules vitamins <i>Chlorella vulgaris</i> carbohydrate antioxidant antitumor |
| title | Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different Vitamins |
| title_full | Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different Vitamins |
| title_fullStr | Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different Vitamins |
| title_full_unstemmed | Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different Vitamins |
| title_short | Assessment of Antioxidant and Anticancer Activities of Microgreen Alga <i>Chlorella vulgaris</i> and Its Blend with Different Vitamins |
| title_sort | assessment of antioxidant and anticancer activities of microgreen alga i chlorella vulgaris i and its blend with different vitamins |
| topic | vitamins <i>Chlorella vulgaris</i> carbohydrate antioxidant antitumor |
| url | https://www.mdpi.com/1420-3049/27/5/1602 |
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