Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence
In this work, the effect of Ag+ on DNA sensitized Tb3+ luminescence was studied initially using the Ag+-specific RNA-cleaving DNAzyme, Ag10c. While we expected to observe luminescence quenching by Ag+, a significant enhancement was produced. Based on this observation, simple DNA oligonucleotide homo...
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MDPI AG
2016-08-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/16/9/1370 |
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| author | Kimberly Kleinke Runjhun Saran Juewen Liu |
| author_facet | Kimberly Kleinke Runjhun Saran Juewen Liu |
| author_sort | Kimberly Kleinke |
| collection | DOAJ |
| description | In this work, the effect of Ag+ on DNA sensitized Tb3+ luminescence was studied initially using the Ag+-specific RNA-cleaving DNAzyme, Ag10c. While we expected to observe luminescence quenching by Ag+, a significant enhancement was produced. Based on this observation, simple DNA oligonucleotide homopolymers were used with systematically varied sequence and length. We discovered that both poly-G and poly-T DNA have a significant emission enhancement by Ag+, while the absolute intensity is stronger with the poly-G DNA, indicating that a G-quadruplex DNA is not required for this enhancement. Using the optimized length of the G7 DNA (an oligo constituted with seven guanines), Ag+ was measured with a detection limit of 57.6 nM. The signaling kinetics, G7 DNA conformation, and the binding affinity of Tb3+ to the DNA in the presence or absence of Ag+ are also studied to reveal the mechanism of emission enhancement. This observation is useful not only for label-free detection of Ag+, but also interesting for the rational design of new biosensors using Tb3+ luminescence. |
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| language | English |
| last_indexed | 2024-04-11T13:14:10Z |
| publishDate | 2016-08-01 |
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| spelling | doaj.art-dcccad0fe5334d16b7cf852c4a5a38b72022-12-22T04:22:27ZengMDPI AGSensors1424-82202016-08-01169137010.3390/s16091370s16091370Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ LuminescenceKimberly Kleinke0Runjhun Saran1Juewen Liu2Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, CanadaDepartment of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, CanadaDepartment of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, CanadaIn this work, the effect of Ag+ on DNA sensitized Tb3+ luminescence was studied initially using the Ag+-specific RNA-cleaving DNAzyme, Ag10c. While we expected to observe luminescence quenching by Ag+, a significant enhancement was produced. Based on this observation, simple DNA oligonucleotide homopolymers were used with systematically varied sequence and length. We discovered that both poly-G and poly-T DNA have a significant emission enhancement by Ag+, while the absolute intensity is stronger with the poly-G DNA, indicating that a G-quadruplex DNA is not required for this enhancement. Using the optimized length of the G7 DNA (an oligo constituted with seven guanines), Ag+ was measured with a detection limit of 57.6 nM. The signaling kinetics, G7 DNA conformation, and the binding affinity of Tb3+ to the DNA in the presence or absence of Ag+ are also studied to reveal the mechanism of emission enhancement. This observation is useful not only for label-free detection of Ag+, but also interesting for the rational design of new biosensors using Tb3+ luminescence.http://www.mdpi.com/1424-8220/16/9/1370aptamersbiosensorsfluorescenceluminescenceterbiumsilver |
| spellingShingle | Kimberly Kleinke Runjhun Saran Juewen Liu Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence Sensors aptamers biosensors fluorescence luminescence terbium silver |
| title | Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence |
| title_full | Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence |
| title_fullStr | Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence |
| title_full_unstemmed | Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence |
| title_short | Label-Free Ag+ Detection by Enhancing DNA Sensitized Tb3+ Luminescence |
| title_sort | label free ag detection by enhancing dna sensitized tb3 luminescence |
| topic | aptamers biosensors fluorescence luminescence terbium silver |
| url | http://www.mdpi.com/1424-8220/16/9/1370 |
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