Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound
Background: Liver fibrosis is a global health problem, and studying its development provides important information to address its treatment. Here, we characterized the effects of an adenosine compound (IFC-305) on preventing fibrosis and liver inflammation. Methods: We studied the impact of IFC-305...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223010077 |
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| author | Mariana Domínguez-López Rebeca Pérez-Cabeza de Vaca Jesús Rafael Rodríguez-Aguilera Nuria Guerrero-Celis Gabriela Velasco-Loyden Victoria Chagoya de Sánchez |
| author_facet | Mariana Domínguez-López Rebeca Pérez-Cabeza de Vaca Jesús Rafael Rodríguez-Aguilera Nuria Guerrero-Celis Gabriela Velasco-Loyden Victoria Chagoya de Sánchez |
| author_sort | Mariana Domínguez-López |
| collection | DOAJ |
| description | Background: Liver fibrosis is a global health problem, and studying its development provides important information to address its treatment. Here, we characterized the effects of an adenosine compound (IFC-305) on preventing fibrosis and liver inflammation. Methods: We studied the impact of IFC-305 on a carbon tetrachloride-induced liver fibrosis model in Wistar male rats at 4, 6, and 8 weeks. The effects were characterized by liver tissue histology, macrophages identification by flow cytometry with CD163+/CD11b/c+ antibodies, hepatic and plasmatic cytokine levels employing MILLIPLEX MAP and ELISA, Col1a1 and Il6 gene expression by RTqPCR, lipoperoxidation by TBARS reaction, and reactive oxygen species using 2'−7'dichlorofluorescin diacetate. Results: CCl4-induced liver fibrosis and inflammation were significantly reduced in rats treated with IFC-305 at 6 and 8 weeks. In addition, we observed diminished expression of Col1a1; a decrease in the inflammatory cytokines IL-1β, IL-6, MCP-1, TNF-α, and IL-4 a; reduction in inflammatory macrophages; inhibition of lipoperoxidation; and ROS production in Kupffer cells. Conclusion: This study showed that IFC-305 can inhibit liver fibrosis establishment by regulating the immune response during CCl4-induced damage. The immunomodulatory action of IFC-305 supports its use as a potential therapeutic strategy for preventing liver fibrosis. |
| first_indexed | 2024-03-12T15:04:48Z |
| format | Article |
| id | doaj.art-dccd85fecd804eb0af08e1808f52933e |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-12T15:04:48Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-dccd85fecd804eb0af08e1808f52933e2023-08-13T04:53:04ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-09-01165115216Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compoundMariana Domínguez-López0Rebeca Pérez-Cabeza de Vaca1Jesús Rafael Rodríguez-Aguilera2Nuria Guerrero-Celis3Gabriela Velasco-Loyden4Victoria Chagoya de Sánchez5Instituto de Fisiología Celular, UNAM, Departamento de Biología Celular y Desarrollo, Laboratorio, Circuito Exterior s/n Ciudad Universitaria, Coyoacán, 04510 México City, MexicoInstituto de Fisiología Celular, UNAM, Departamento de Biología Celular y Desarrollo, Laboratorio, Circuito Exterior s/n Ciudad Universitaria, Coyoacán, 04510 México City, Mexico; Tecnológico de Monterrey, Escuela de Medicina y Ciencias de la Salud and The Institute for Obesity Research, Monterrey C.P 64710, MexicoInstituto de Fisiología Celular, UNAM, Departamento de Biología Celular y Desarrollo, Laboratorio, Circuito Exterior s/n Ciudad Universitaria, Coyoacán, 04510 México City, MexicoInstituto de Fisiología Celular, UNAM, Departamento de Biología Celular y Desarrollo, Laboratorio, Circuito Exterior s/n Ciudad Universitaria, Coyoacán, 04510 México City, MexicoInstituto de Fisiología Celular, UNAM, Departamento de Biología Celular y Desarrollo, Laboratorio, Circuito Exterior s/n Ciudad Universitaria, Coyoacán, 04510 México City, MexicoInstituto de Fisiología Celular, UNAM, Departamento de Biología Celular y Desarrollo, Laboratorio, Circuito Exterior s/n Ciudad Universitaria, Coyoacán, 04510 México City, Mexico; Corresponding author.Background: Liver fibrosis is a global health problem, and studying its development provides important information to address its treatment. Here, we characterized the effects of an adenosine compound (IFC-305) on preventing fibrosis and liver inflammation. Methods: We studied the impact of IFC-305 on a carbon tetrachloride-induced liver fibrosis model in Wistar male rats at 4, 6, and 8 weeks. The effects were characterized by liver tissue histology, macrophages identification by flow cytometry with CD163+/CD11b/c+ antibodies, hepatic and plasmatic cytokine levels employing MILLIPLEX MAP and ELISA, Col1a1 and Il6 gene expression by RTqPCR, lipoperoxidation by TBARS reaction, and reactive oxygen species using 2'−7'dichlorofluorescin diacetate. Results: CCl4-induced liver fibrosis and inflammation were significantly reduced in rats treated with IFC-305 at 6 and 8 weeks. In addition, we observed diminished expression of Col1a1; a decrease in the inflammatory cytokines IL-1β, IL-6, MCP-1, TNF-α, and IL-4 a; reduction in inflammatory macrophages; inhibition of lipoperoxidation; and ROS production in Kupffer cells. Conclusion: This study showed that IFC-305 can inhibit liver fibrosis establishment by regulating the immune response during CCl4-induced damage. The immunomodulatory action of IFC-305 supports its use as a potential therapeutic strategy for preventing liver fibrosis.http://www.sciencedirect.com/science/article/pii/S0753332223010077Liver fibrosisCarbon tetrachlorideIFC-305Collagen fibersCytokinesKupffer cells |
| spellingShingle | Mariana Domínguez-López Rebeca Pérez-Cabeza de Vaca Jesús Rafael Rodríguez-Aguilera Nuria Guerrero-Celis Gabriela Velasco-Loyden Victoria Chagoya de Sánchez Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound Biomedicine & Pharmacotherapy Liver fibrosis Carbon tetrachloride IFC-305 Collagen fibers Cytokines Kupffer cells |
| title | Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound |
| title_full | Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound |
| title_fullStr | Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound |
| title_full_unstemmed | Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound |
| title_short | Liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound |
| title_sort | liver fibrotic development is reduced through inflammation prevention by an adenosine derivative compound |
| topic | Liver fibrosis Carbon tetrachloride IFC-305 Collagen fibers Cytokines Kupffer cells |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223010077 |
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