DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli
Some organisms have shown the ability to naturally survive in extreme environments, even outer space. Some of these have natural mechanisms to resist severe DNA damage from conditions such as ionizing and non-ionizing radiation, extreme temperatures, and low pressures or vacuum. A good example can b...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.789668/full |
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| author | Jaume Puig Jaume Puig Nastassia Knödlseder Nastassia Knödlseder Jaume Quera Jaume Quera Jaume Quera Manuel Algara Manuel Algara Manuel Algara Marc Güell Marc Güell |
| author_facet | Jaume Puig Jaume Puig Nastassia Knödlseder Nastassia Knödlseder Jaume Quera Jaume Quera Jaume Quera Manuel Algara Manuel Algara Manuel Algara Marc Güell Marc Güell |
| author_sort | Jaume Puig |
| collection | DOAJ |
| description | Some organisms have shown the ability to naturally survive in extreme environments, even outer space. Some of these have natural mechanisms to resist severe DNA damage from conditions such as ionizing and non-ionizing radiation, extreme temperatures, and low pressures or vacuum. A good example can be found in Deinococcus radiodurans, which was exposed to severe conditions such as those listed in the Exposure Facility of the International Space Station (ISS) for up to three years. Another example are tardigrades (Ramazzottius varieornatus) which are some of the most resilient animals known. In this study, the survival under simulated Low earth Orbit (LEO) environmental conditions was tested in Escherichia coli. The radiation resistance of this bacteria was enhanced using the Dsup gene from R. varieornatus, and two more genes from D. radiodurans involved in DNA damage repair, RecA and uvrD. The enhanced survival to wide ranges of temperatures and low pressures was then tested in the new strains. This research constitutes a first step in the creation of new bacterial strains engineered to survive severe conditions and adapting existing species for their survival in remote environments, including extra-terrestrial habitats. These strains could be key for the development of environments hospitable to life and could be of use for ecological restoration and space exploration. In addition, studying the efficacy and the functioning of the DNA repair mechanisms used in this study could be beneficial for medical and life sciences engineering. |
| first_indexed | 2024-12-23T14:18:33Z |
| format | Article |
| id | doaj.art-dcce527dc84a48b2aab0c91b1e65e9fa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-23T14:18:33Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dcce527dc84a48b2aab0c91b1e65e9fa2022-12-21T17:43:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-12-011210.3389/fmicb.2021.789668789668DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coliJaume Puig0Jaume Puig1Nastassia Knödlseder2Nastassia Knödlseder3Jaume Quera4Jaume Quera5Jaume Quera6Manuel Algara7Manuel Algara8Manuel Algara9Marc Güell10Marc Güell11Translational Synthetic Biology Laboratory, Experimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainExperimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainTranslational Synthetic Biology Laboratory, Experimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainExperimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainExperimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainRadiation Oncology Department, Hospital del Mar, Parc de Salut Mar, Barcelona, SpainIMIM Hospital del Mar Medical Research Institute, Barcelona, SpainExperimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainRadiation Oncology Department, Hospital del Mar, Parc de Salut Mar, Barcelona, SpainIMIM Hospital del Mar Medical Research Institute, Barcelona, SpainTranslational Synthetic Biology Laboratory, Experimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainExperimental and Health Sciences Department, Universitat Pompeu Fabra, Barcelona, SpainSome organisms have shown the ability to naturally survive in extreme environments, even outer space. Some of these have natural mechanisms to resist severe DNA damage from conditions such as ionizing and non-ionizing radiation, extreme temperatures, and low pressures or vacuum. A good example can be found in Deinococcus radiodurans, which was exposed to severe conditions such as those listed in the Exposure Facility of the International Space Station (ISS) for up to three years. Another example are tardigrades (Ramazzottius varieornatus) which are some of the most resilient animals known. In this study, the survival under simulated Low earth Orbit (LEO) environmental conditions was tested in Escherichia coli. The radiation resistance of this bacteria was enhanced using the Dsup gene from R. varieornatus, and two more genes from D. radiodurans involved in DNA damage repair, RecA and uvrD. The enhanced survival to wide ranges of temperatures and low pressures was then tested in the new strains. This research constitutes a first step in the creation of new bacterial strains engineered to survive severe conditions and adapting existing species for their survival in remote environments, including extra-terrestrial habitats. These strains could be key for the development of environments hospitable to life and could be of use for ecological restoration and space exploration. In addition, studying the efficacy and the functioning of the DNA repair mechanisms used in this study could be beneficial for medical and life sciences engineering.https://www.frontiersin.org/articles/10.3389/fmicb.2021.789668/fullastrobiologyDNA repairdirected molecular evolutionUV survivalradiation resistancelow pressure |
| spellingShingle | Jaume Puig Jaume Puig Nastassia Knödlseder Nastassia Knödlseder Jaume Quera Jaume Quera Jaume Quera Manuel Algara Manuel Algara Manuel Algara Marc Güell Marc Güell DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli Frontiers in Microbiology astrobiology DNA repair directed molecular evolution UV survival radiation resistance low pressure |
| title | DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli |
| title_full | DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli |
| title_fullStr | DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli |
| title_full_unstemmed | DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli |
| title_short | DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli |
| title_sort | dna damage protection for enhanced bacterial survival under simulated low earth orbit environmental conditions in escherichia coli |
| topic | astrobiology DNA repair directed molecular evolution UV survival radiation resistance low pressure |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.789668/full |
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