A MAD7‐based genome editing system for Escherichia coli
Abstract A broad variety of biomolecules is industrially produced in bacteria and yeasts. These microbial expression hosts can be optimized through genetic engineering using CRISPR tools. Here, we designed and characterized such a modular genome editing system based on the Cas12a‐like RNA‐guided nuc...
Main Authors: | Markus Mund, Wadim Weber, Daniel Degreif, Christoph Schiklenk |
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Format: | Article |
Language: | English |
Published: |
Wiley
2023-05-01
|
Series: | Microbial Biotechnology |
Online Access: | https://doi.org/10.1111/1751-7915.14234 |
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