Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases
The counter-immunoelectrophoresis (CIE) is a highly specific, simple and rapid method, which does not require expensive equipment, input materials and long-term staff training as compared with other express methods of viral infections diagnostic. Despite the CIE high informativeness, the results in...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2021-02-01
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| Series: | The Ukrainian Biochemical Journal |
| Subjects: | |
| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2021/02/Ostapiv_1_21.pdf |
| _version_ | 1797641857072627712 |
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| author | D. D. Ostapiv N. V. Kuz’mina М. R. Kozak Shan Hu V. V. Vlizlo I. Ya. Kotsiumbas S. M. Varvarenko V. Ya. Samaryk N. G. Nosova M. V. Yakoviv |
| author_facet | D. D. Ostapiv N. V. Kuz’mina М. R. Kozak Shan Hu V. V. Vlizlo I. Ya. Kotsiumbas S. M. Varvarenko V. Ya. Samaryk N. G. Nosova M. V. Yakoviv |
| author_sort | D. D. Ostapiv |
| collection | DOAJ |
| description | The counter-immunoelectrophoresis (CIE) is a highly specific, simple and rapid method, which does not require expensive equipment, input materials and long-term staff training as compared with other express methods of viral infections diagnostic. Despite the CIE high informativeness, the results in practice can be doubtful at times. Therefore, the purpose of the study was to improve CIE method by strengthening the antigen-antibody interaction with the use of synthesized amphiphilic fluorescein-containing copolymer. It was demonstrated that the use of the fluorescein copolymer -antigen complex enhanced the interaction with serum/plasma antibodies of rabbits immunized against hemorrhagic disease and increased protein content in the area of precipitation. This effect was more pronounced when more hydrophobic copolymer containing 5.87% fluorescein was introduced into the complex with antigen. The ability of the amphiphilic fluorescein copolymer to enhance the antigen-antibody interaction and the ability to visualize the fluorescent precipitation zone may increase CIE effectiveness for express diagnostic of infectious diseases. |
| first_indexed | 2024-03-11T13:51:39Z |
| format | Article |
| id | doaj.art-dcda5ca3dfec42c79436829172534297 |
| institution | Directory Open Access Journal |
| issn | 2409-4943 2413-5003 |
| language | English |
| last_indexed | 2024-03-11T13:51:39Z |
| publishDate | 2021-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj.art-dcda5ca3dfec42c794368291725342972023-11-02T09:07:33ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032021-02-0193110411210.15407/ubj93.01.104Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseasesD. D. Ostapiv0https://orcid.org/0000-0001-8112-5398N. V. Kuz’mina1https://orcid.org/0000-0002-3424-325XМ. R. Kozak2https://orcid.org/0000-0002-2271-8529Shan Hu3https://orcid.org/0000-0001-6032-1496V. V. Vlizlo4https://orcid.org/0000-0002-7685-9853I. Ya. Kotsiumbas5https://orcid.org/0000-0002-1592-6258S. M. Varvarenko6https://orcid.org/0000-0001-7374-7787V. Ya. Samaryk7https://orcid.org/0000-0078-0167-3646N. G. Nosova8https://orcid.org/0000-0002-4682-5388M. V. Yakoviv9https://orcid.org/0000-0001-9406-5187Institute of Animal Biology, NAAS of Ukraine, Lviv, UkraineInstitute of Animal Biology, NAAS of Ukraine, Lviv, UkraineInstitute of Animal Biology, NAAS of Ukraine, Lviv, UkraineQingdao Agricultural University, Qingdao, ChinaState Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, UkraineState Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, UkraineLviv Polytechnic National University, Lviv, UkraineLviv Polytechnic National University, Lviv, UkraineLviv Polytechnic National University, Lviv, UkraineLviv Polytechnic National University, Lviv, UkraineThe counter-immunoelectrophoresis (CIE) is a highly specific, simple and rapid method, which does not require expensive equipment, input materials and long-term staff training as compared with other express methods of viral infections diagnostic. Despite the CIE high informativeness, the results in practice can be doubtful at times. Therefore, the purpose of the study was to improve CIE method by strengthening the antigen-antibody interaction with the use of synthesized amphiphilic fluorescein-containing copolymer. It was demonstrated that the use of the fluorescein copolymer -antigen complex enhanced the interaction with serum/plasma antibodies of rabbits immunized against hemorrhagic disease and increased protein content in the area of precipitation. This effect was more pronounced when more hydrophobic copolymer containing 5.87% fluorescein was introduced into the complex with antigen. The ability of the amphiphilic fluorescein copolymer to enhance the antigen-antibody interaction and the ability to visualize the fluorescent precipitation zone may increase CIE effectiveness for express diagnostic of infectious diseases.http://ukrbiochemjournal.org/wp-content/uploads/2021/02/Ostapiv_1_21.pdfcounter-immunoelectrophoresisamphiphilic fluorescein copolymerrapid diagnosticsrabbit hemorrhagic disease |
| spellingShingle | D. D. Ostapiv N. V. Kuz’mina М. R. Kozak Shan Hu V. V. Vlizlo I. Ya. Kotsiumbas S. M. Varvarenko V. Ya. Samaryk N. G. Nosova M. V. Yakoviv Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases The Ukrainian Biochemical Journal counter-immunoelectrophoresis amphiphilic fluorescein copolymer rapid diagnostics rabbit hemorrhagic disease |
| title | Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases |
| title_full | Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases |
| title_fullStr | Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases |
| title_full_unstemmed | Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases |
| title_short | Application of fluorescein copolymer to improve the efficiency of counter-immunoelectrophoresis for diagnostics of animal infectious diseases |
| title_sort | application of fluorescein copolymer to improve the efficiency of counter immunoelectrophoresis for diagnostics of animal infectious diseases |
| topic | counter-immunoelectrophoresis amphiphilic fluorescein copolymer rapid diagnostics rabbit hemorrhagic disease |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2021/02/Ostapiv_1_21.pdf |
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