Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering
Abstract Regeneration of bone lost by trauma, diseases and aging, and restoration of its load‐bearing function are major clinical challenges. Hydroxyapatite (HA) is a clinically proven scaffold material for bone grafting, but the random‐pore structure limits the homing of the cells inside the graft...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-06-01
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| Series: | MedComm – Future Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mef2.17 |
| _version_ | 1811166489853558784 |
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| author | Gayathry G. Athira R. K. Anju M. S. Anil Kumar P. R. Harikrishna Varma P. R. Naresh Kasoju Manoj Komath |
| author_facet | Gayathry G. Athira R. K. Anju M. S. Anil Kumar P. R. Harikrishna Varma P. R. Naresh Kasoju Manoj Komath |
| author_sort | Gayathry G. |
| collection | DOAJ |
| description | Abstract Regeneration of bone lost by trauma, diseases and aging, and restoration of its load‐bearing function are major clinical challenges. Hydroxyapatite (HA) is a clinically proven scaffold material for bone grafting, but the random‐pore structure limits the homing of the cells inside the graft and the bone regeneration progresses with the resorption of the graft material. This work is based on the hypothesis that aligned through pores in the graft will lead to a faster healing by homing the local cells inside and provide a better environment for new bone formation through the graft structure. The investigation was done using aligned porous HA scaffolds seeded with human Wharton's jelly‐derived mesenchymal stem cells (hWJ‐MSCs) and cultured in a multiwell format bioreactor setup. The cell adhesion was studied by microscopy, cell proliferation was evaluated by Alamar blue assay and osteogenic differentiation was confirmed by biochemical and molecular assays. The results indicate that the hWJ‐MSCs infiltrated through the aligned porous network of the scaffold, proliferated well when cultured in the expansion medium, and differentiated into osteogenic lineage when cultured in the differentiation medium. |
| first_indexed | 2024-04-10T15:53:18Z |
| format | Article |
| id | doaj.art-dcdb192867204f89ae36a698f65d82ec |
| institution | Directory Open Access Journal |
| issn | 2769-6456 |
| language | English |
| last_indexed | 2024-04-10T15:53:18Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm – Future Medicine |
| spelling | doaj.art-dcdb192867204f89ae36a698f65d82ec2023-02-10T23:20:11ZengWileyMedComm – Future Medicine2769-64562022-06-0111n/an/a10.1002/mef2.17Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineeringGayathry G.0Athira R. K.1Anju M. S.2Anil Kumar P. R.3Harikrishna Varma P. R.4Naresh Kasoju5Manoj Komath6Department of Biomaterial Science and Technology, Division of Bioceramics Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology Thiruvananthapuram Kerala IndiaDepartment of Applied Biology, Division of Tissue Culture, Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Science and Technology Thiruvananthapuram Kerala IndiaDepartment of Applied Biology, Division of Tissue Culture, Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Science and Technology Thiruvananthapuram Kerala IndiaDepartment of Applied Biology, Division of Tissue Culture, Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Science and Technology Thiruvananthapuram Kerala IndiaDepartment of Biomaterial Science and Technology, Division of Bioceramics Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology Thiruvananthapuram Kerala IndiaDepartment of Applied Biology, Division of Tissue Culture, Biomedical Technology Wing Sree Chitra Tirunal Institute for Medical Science and Technology Thiruvananthapuram Kerala IndiaDepartment of Biomaterial Science and Technology, Division of Bioceramics Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology Thiruvananthapuram Kerala IndiaAbstract Regeneration of bone lost by trauma, diseases and aging, and restoration of its load‐bearing function are major clinical challenges. Hydroxyapatite (HA) is a clinically proven scaffold material for bone grafting, but the random‐pore structure limits the homing of the cells inside the graft and the bone regeneration progresses with the resorption of the graft material. This work is based on the hypothesis that aligned through pores in the graft will lead to a faster healing by homing the local cells inside and provide a better environment for new bone formation through the graft structure. The investigation was done using aligned porous HA scaffolds seeded with human Wharton's jelly‐derived mesenchymal stem cells (hWJ‐MSCs) and cultured in a multiwell format bioreactor setup. The cell adhesion was studied by microscopy, cell proliferation was evaluated by Alamar blue assay and osteogenic differentiation was confirmed by biochemical and molecular assays. The results indicate that the hWJ‐MSCs infiltrated through the aligned porous network of the scaffold, proliferated well when cultured in the expansion medium, and differentiated into osteogenic lineage when cultured in the differentiation medium.https://doi.org/10.1002/mef2.17bioceramicsbioreactorsbone defectsosteogenesisregenerative medicinestem cells |
| spellingShingle | Gayathry G. Athira R. K. Anju M. S. Anil Kumar P. R. Harikrishna Varma P. R. Naresh Kasoju Manoj Komath Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering MedComm – Future Medicine bioceramics bioreactors bone defects osteogenesis regenerative medicine stem cells |
| title | Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering |
| title_full | Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering |
| title_fullStr | Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering |
| title_full_unstemmed | Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering |
| title_short | Mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering |
| title_sort | mesenchymal stem cell culture in aligned porous hydroxyapatite scaffolds using a multiwell plate bioreactor for bone tissue engineering |
| topic | bioceramics bioreactors bone defects osteogenesis regenerative medicine stem cells |
| url | https://doi.org/10.1002/mef2.17 |
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