Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy
Renal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold stand...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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International Union of Crystallography
2022-01-01
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| Series: | Journal of Synchrotron Radiation |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S1600577521011371 |
| _version_ | 1818772473652445184 |
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| author | Emmanuel Estève David Buob Frédéric Jamme Chantal Jouanneau Slavka Kascakova Jean-Philippe Haymann Emmanuel Letavernier Louise Galmiche Pierre Ronco Michel Daudon Dominique Bazin Matthieu Réfrégiers |
| author_facet | Emmanuel Estève David Buob Frédéric Jamme Chantal Jouanneau Slavka Kascakova Jean-Philippe Haymann Emmanuel Letavernier Louise Galmiche Pierre Ronco Michel Daudon Dominique Bazin Matthieu Réfrégiers |
| author_sort | Emmanuel Estève |
| collection | DOAJ |
| description | Renal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold standard. The fluorescence spectrum of synthetic mono-, di- and tri-hydrated calcium oxalate was investigated using a microspectrometer coupled to the synchrotron UV beamline DISCO, Synchrotron SOLEIL, France. The obtained spectra were used to detect oxalocalcic crystals in a case control study of 42 human kidney biopsies including 19 renal oxalosis due to primary (PHO, n = 11) and secondary hyperoxaluria (SHO, n = 8), seven samples from PHO patients who received combined kidney and liver transplants, and 16 controls. For all oxalocalcic hydrates samples, a fluorescence signal is detected at 420 nm. These spectra were used to identify standard oxalocalcic crystals in patients with PHO or SHO. They also revealed micrometric crystallites as well as non-aggregated oxalate accumulation in tubular cells. A nine-points histological score was established for the diagnosis of renal oxalosis with 100% specificity (76–100) and a 73% sensitivity (43–90). Oxalate tubular accumulation and higher histological score were correlated to lower estimated glomerular filtration rate and higher urinary oxalate over creatinine ratio. |
| first_indexed | 2024-12-18T10:09:55Z |
| format | Article |
| id | doaj.art-dce272ea25564f1cbb15fe3d3da852f1 |
| institution | Directory Open Access Journal |
| issn | 1600-5775 |
| language | English |
| last_indexed | 2024-12-18T10:09:55Z |
| publishDate | 2022-01-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | Journal of Synchrotron Radiation |
| spelling | doaj.art-dce272ea25564f1cbb15fe3d3da852f12022-12-21T21:11:27ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752022-01-0129121422310.1107/S1600577521011371yn5078Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopyEmmanuel Estève0David Buob1Frédéric Jamme2Chantal Jouanneau3Slavka Kascakova4Jean-Philippe Haymann5Emmanuel Letavernier6Louise Galmiche7Pierre Ronco8Michel Daudon9Dominique Bazin10Matthieu Réfrégiers11Sorbonne Université, UPMC Paris 06, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 1155, F-75020 Paris, FranceSorbonne Université, UPMC Paris 06, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 1155, F-75020 Paris, FranceSynchrotron SOLEIL, DISCO Beamline, L'Orme des Merisiers, Saint-Aubin, 91192 Gif sur Yvette, FranceSorbonne Université, UPMC Paris 06, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 1155, F-75020 Paris, FranceSynchrotron SOLEIL, DISCO Beamline, L'Orme des Merisiers, Saint-Aubin, 91192 Gif sur Yvette, FranceSorbonne Université, UPMC Paris 06, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 1155, F-75020 Paris, FranceSorbonne Université, UPMC Paris 06, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 1155, F-75020 Paris, FrancePathology Department, Necker-Enfants Malades Hospital, Public Assistance-Hospitals of Paris, Université Paris, 75015 Paris, FranceSorbonne Université, UPMC Paris 06, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 1155, F-75020 Paris, FranceSorbonne Université, UPMC Paris 06, Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 1155, F-75020 Paris, FranceLaboratoire de Physique des Solides, CNRS UMR8502, Université Paris Saclay, Orsay, FranceSynchrotron SOLEIL, DISCO Beamline, L'Orme des Merisiers, Saint-Aubin, 91192 Gif sur Yvette, FranceRenal oxalosis is a rare cause of renal failure whose diagnosis can be challenging. Synchrotron deep ultraviolet (UV) fluorescence was assayed to improve oxalosis detection on kidney biopsies spatial resolution and sensitivity compared with the Fourier transform infrared microspectroscopy gold standard. The fluorescence spectrum of synthetic mono-, di- and tri-hydrated calcium oxalate was investigated using a microspectrometer coupled to the synchrotron UV beamline DISCO, Synchrotron SOLEIL, France. The obtained spectra were used to detect oxalocalcic crystals in a case control study of 42 human kidney biopsies including 19 renal oxalosis due to primary (PHO, n = 11) and secondary hyperoxaluria (SHO, n = 8), seven samples from PHO patients who received combined kidney and liver transplants, and 16 controls. For all oxalocalcic hydrates samples, a fluorescence signal is detected at 420 nm. These spectra were used to identify standard oxalocalcic crystals in patients with PHO or SHO. They also revealed micrometric crystallites as well as non-aggregated oxalate accumulation in tubular cells. A nine-points histological score was established for the diagnosis of renal oxalosis with 100% specificity (76–100) and a 73% sensitivity (43–90). Oxalate tubular accumulation and higher histological score were correlated to lower estimated glomerular filtration rate and higher urinary oxalate over creatinine ratio.http://scripts.iucr.org/cgi-bin/paper?S1600577521011371oxalateoxalosisdeep ultraviolet microscopysynchrotron diagnosiskidney biopsiessynchrotron diagnosis |
| spellingShingle | Emmanuel Estève David Buob Frédéric Jamme Chantal Jouanneau Slavka Kascakova Jean-Philippe Haymann Emmanuel Letavernier Louise Galmiche Pierre Ronco Michel Daudon Dominique Bazin Matthieu Réfrégiers Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy Journal of Synchrotron Radiation oxalate oxalosis deep ultraviolet microscopy synchrotron diagnosis kidney biopsies synchrotron diagnosis |
| title | Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy |
| title_full | Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy |
| title_fullStr | Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy |
| title_full_unstemmed | Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy |
| title_short | Detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy |
| title_sort | detection and localization of calcium oxalate in kidney using synchrotron deep ultraviolet fluorescence microscopy |
| topic | oxalate oxalosis deep ultraviolet microscopy synchrotron diagnosis kidney biopsies synchrotron diagnosis |
| url | http://scripts.iucr.org/cgi-bin/paper?S1600577521011371 |
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