Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral Leishmaniasis
Background: To evaluate immunotherapy against canine visceral leishmaniasis, Leishmania major antigen and heat-killed Mycobacterium vaccae (SRL172) were used as stimulators of immune defense mechanisms and the results were compared with standard chemotherapy meglumine antimoniate. Methods: Ninet...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2011-09-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/185 |
| _version_ | 1798027422186078208 |
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| author | Sh Jamshidi R Avizeh M Mohebali S Bokaie |
| author_facet | Sh Jamshidi R Avizeh M Mohebali S Bokaie |
| author_sort | Sh Jamshidi |
| collection | DOAJ |
| description | Background: To evaluate immunotherapy against canine visceral leishmaniasis, Leishmania major antigen and heat-killed Mycobacterium vaccae (SRL172) were used as stimulators of immune defense mechanisms and the results were compared with standard chemotherapy meglumine antimoniate.
Methods: Nineteen mongrel dogs aging 1-3 years old were used in this experiment. Infection was carried out in 15 out of 19 dogs using L. infantum, isolated from a naturally infected poly-symptomatic dog.
Results: All the cases showed positive serologic results by direct agglutination test during 30-60 days following inoculation. In the first group, which was under chemotherapy (GlucantimeR), one of the members showed recurrence of the disease despite rapid effect of the therapeutic protocol. Immunotherapy using SRL172 caused complete cleaning of the parasite in group 2, but the speed was less than Glucantime. Immunotherapy using L. major antigen combined with M. vaccae in group 3 and combine administration of immunotherapy and chemotherapy in group 4 both were with relapsing of one case in each group. Group 5 and 6 were consisted of positive and negative control dogs, respectively.
Conclusion: Immunotherapy seems to be an adjuvant in treatment of canine leishmaniasis but it needs more investigation for final confirmation. |
| first_indexed | 2024-04-11T18:51:11Z |
| format | Article |
| id | doaj.art-dce46239f3b744d7b0f73dde9445b7b4 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-11T18:51:11Z |
| publishDate | 2011-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-dce46239f3b744d7b0f73dde9445b7b42022-12-22T04:08:23ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2011-09-0163Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral LeishmaniasisSh Jamshidi0R Avizeh1M Mohebali2S Bokaie3Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, IranDept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranBackground: To evaluate immunotherapy against canine visceral leishmaniasis, Leishmania major antigen and heat-killed Mycobacterium vaccae (SRL172) were used as stimulators of immune defense mechanisms and the results were compared with standard chemotherapy meglumine antimoniate. Methods: Nineteen mongrel dogs aging 1-3 years old were used in this experiment. Infection was carried out in 15 out of 19 dogs using L. infantum, isolated from a naturally infected poly-symptomatic dog. Results: All the cases showed positive serologic results by direct agglutination test during 30-60 days following inoculation. In the first group, which was under chemotherapy (GlucantimeR), one of the members showed recurrence of the disease despite rapid effect of the therapeutic protocol. Immunotherapy using SRL172 caused complete cleaning of the parasite in group 2, but the speed was less than Glucantime. Immunotherapy using L. major antigen combined with M. vaccae in group 3 and combine administration of immunotherapy and chemotherapy in group 4 both were with relapsing of one case in each group. Group 5 and 6 were consisted of positive and negative control dogs, respectively. Conclusion: Immunotherapy seems to be an adjuvant in treatment of canine leishmaniasis but it needs more investigation for final confirmation.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/185Canine visceral leishmaniasisImmunotherapyGlucantimeMycobacterium vaccae |
| spellingShingle | Sh Jamshidi R Avizeh M Mohebali S Bokaie Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral Leishmaniasis Iranian Journal of Parasitology Canine visceral leishmaniasis Immunotherapy Glucantime Mycobacterium vaccae |
| title | Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral Leishmaniasis |
| title_full | Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral Leishmaniasis |
| title_fullStr | Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral Leishmaniasis |
| title_full_unstemmed | Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral Leishmaniasis |
| title_short | Immunotherapy Using Autoclaved L. major Antigens and M. vaccae with Meglumine Antimoniate, for the Treatment of Experimental Canine Visceral Leishmaniasis |
| title_sort | immunotherapy using autoclaved l major antigens and m vaccae with meglumine antimoniate for the treatment of experimental canine visceral leishmaniasis |
| topic | Canine visceral leishmaniasis Immunotherapy Glucantime Mycobacterium vaccae |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/185 |
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