Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience
Mycolic acids analysis by thin-layer chromatography (TLC) has been employed by several laboratories worldwide as a method for fast identification of mycobacteria. This method was introduced in Brazil by our laboratory in 1992 as a routine identification technique. Up to the present, 861 strains isol...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1998-11-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000600019&tlng=en |
| _version_ | 1827844812003344384 |
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| author | Clarice Queico Fujimura Leite Clovis Wesley Oliveira de Souza Sergio Roberto de Andrade Leite |
| author_facet | Clarice Queico Fujimura Leite Clovis Wesley Oliveira de Souza Sergio Roberto de Andrade Leite |
| author_sort | Clarice Queico Fujimura Leite |
| collection | DOAJ |
| description | Mycolic acids analysis by thin-layer chromatography (TLC) has been employed by several laboratories worldwide as a method for fast identification of mycobacteria. This method was introduced in Brazil by our laboratory in 1992 as a routine identification technique. Up to the present, 861 strains isolated were identified by mycolic acids TLC and by standard biochemical tests; 61% out of these strains came as clinical samples, 4% isolated from frogs and 35% as environmental samples. Mycobacterium tuberculosis strains identified by classical methods were confirmed by their mycolic acids contents (I, III and IV). The method allowed earlier differentiation of M. avium complex - MAC (mycolic acids I, IV and VI) from M. simiae (acids I, II and IV), both with similar biochemical properties. The method also permitted to distinguish M. fortuitum (acids I and V) from M. chelonae (acids I and II) , and to detect mixed mycobacterial infections cases as M. tuberculosis with MAC and M. fortuitum with MAC. Concluding, four years experience shows that mycolic acids TLC is an easy, reliable, fast and inexpensive method, an important tool to put together conventional mycobacteria identification methods. |
| first_indexed | 2024-03-12T08:47:15Z |
| format | Article |
| id | doaj.art-dce483cf442241499b633f57f606e5e0 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:47:15Z |
| publishDate | 1998-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-dce483cf442241499b633f57f606e5e02023-09-02T16:25:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80601998-11-0193680180510.1590/S0074-02761998000600019Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of ExperienceClarice Queico Fujimura Leite0Clovis Wesley Oliveira de Souza1Sergio Roberto de Andrade Leite2Universidade Estadual PaulistaUniversidade Federal de São CarlosUniversidade Estadual Paulista, AraraquaraMycolic acids analysis by thin-layer chromatography (TLC) has been employed by several laboratories worldwide as a method for fast identification of mycobacteria. This method was introduced in Brazil by our laboratory in 1992 as a routine identification technique. Up to the present, 861 strains isolated were identified by mycolic acids TLC and by standard biochemical tests; 61% out of these strains came as clinical samples, 4% isolated from frogs and 35% as environmental samples. Mycobacterium tuberculosis strains identified by classical methods were confirmed by their mycolic acids contents (I, III and IV). The method allowed earlier differentiation of M. avium complex - MAC (mycolic acids I, IV and VI) from M. simiae (acids I, II and IV), both with similar biochemical properties. The method also permitted to distinguish M. fortuitum (acids I and V) from M. chelonae (acids I and II) , and to detect mixed mycobacterial infections cases as M. tuberculosis with MAC and M. fortuitum with MAC. Concluding, four years experience shows that mycolic acids TLC is an easy, reliable, fast and inexpensive method, an important tool to put together conventional mycobacteria identification methods.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000600019&tlng=enmycobacteria identificationmycolic acidsthin layer chromatography |
| spellingShingle | Clarice Queico Fujimura Leite Clovis Wesley Oliveira de Souza Sergio Roberto de Andrade Leite Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience Memorias do Instituto Oswaldo Cruz mycobacteria identification mycolic acids thin layer chromatography |
| title | Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience |
| title_full | Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience |
| title_fullStr | Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience |
| title_full_unstemmed | Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience |
| title_short | Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience |
| title_sort | identification of mycobacteria by thin layer chromatographic analysis of mycolic acids and conventional biochemical method four years of experience |
| topic | mycobacteria identification mycolic acids thin layer chromatography |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000600019&tlng=en |
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