Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil
Eight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from orange, Citrus sp.). The influence of inorga...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Mato Grosso do Sul
2018-12-01
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| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | https://periodicos.ufms.br/index.php/orbital/article/view/16038 |
| _version_ | 1797946873859801088 |
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| author | João Paulo Sacchetto Arnaldo F. da Silva Filho Maria I. Rezende Ieda Scarminio Marco A. Z. Ayub Ellen C. Giese Robert F. H. Dekker Aneli Melo Barbosa-Dekker |
| author_facet | João Paulo Sacchetto Arnaldo F. da Silva Filho Maria I. Rezende Ieda Scarminio Marco A. Z. Ayub Ellen C. Giese Robert F. H. Dekker Aneli Melo Barbosa-Dekker |
| author_sort | João Paulo Sacchetto |
| collection | DOAJ |
| description | Eight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from orange, Citrus sp.). The influence of inorganic nitrogen (NH4Cl, NaNO3, (NH4)2SO4, and NH4NO3), organic nitrogen (yeast extract (YE), urea, peptone, and corn steep liquor) sources, inorganic phosphate (KH2PO4, Na2HPO4, NaH2PO4, K2HPO4), as well as increasing concentrations of copper (from zero to 100 µg mL-1), as nutritional requirements for enhancing laccase production by B. rhodina MAMB-05 were also evaluated. Soybean oil was the only carbon source in a central composite factorialdesign (23) to optimize laccase production with the selected variables NaNO3, KH2PO4 and copper (CuSO4.5H2O), using the response surface methodology. The optimal conditions obtained were NaNO3 (100 g L-1), KH2PO4 (125 g L-1) and 50 µg mL-1 of CuSO4.5H2O. The specific laccase activity obtained (2.95 U mg-1) in the validation experiments using the optimal condition for laccase production by B. rhodina MAMB-05 was in agreement with the predicted value (3.07 U mg-1), and demonstrated the fitness of the proposed model.
DOI: http://dx.doi.org/10.17807/orbital.v10i7.1206
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| first_indexed | 2024-04-10T21:17:57Z |
| format | Article |
| id | doaj.art-dce6be6de1a04f5a9ef06679bc48d6e2 |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-04-10T21:17:57Z |
| publishDate | 2018-12-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-dce6be6de1a04f5a9ef06679bc48d6e22023-01-20T10:53:36ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282018-12-01107Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean OilJoão Paulo Sacchetto0Arnaldo F. da Silva Filho1Maria I. Rezende2Ieda Scarminio3Marco A. Z. Ayub4Ellen C. Giese5Robert F. H. Dekker6Aneli Melo Barbosa-Dekker7Universidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Federal do Rio Grande do SulCentro de Tecnologia Mineral, CETEMPrograma de Pós-Graduação em Engenharia Ambiental, Universidade Tecnológica Federal do ParanáUniversidade Estadual de LondrinaEight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from orange, Citrus sp.). The influence of inorganic nitrogen (NH4Cl, NaNO3, (NH4)2SO4, and NH4NO3), organic nitrogen (yeast extract (YE), urea, peptone, and corn steep liquor) sources, inorganic phosphate (KH2PO4, Na2HPO4, NaH2PO4, K2HPO4), as well as increasing concentrations of copper (from zero to 100 µg mL-1), as nutritional requirements for enhancing laccase production by B. rhodina MAMB-05 were also evaluated. Soybean oil was the only carbon source in a central composite factorialdesign (23) to optimize laccase production with the selected variables NaNO3, KH2PO4 and copper (CuSO4.5H2O), using the response surface methodology. The optimal conditions obtained were NaNO3 (100 g L-1), KH2PO4 (125 g L-1) and 50 µg mL-1 of CuSO4.5H2O. The specific laccase activity obtained (2.95 U mg-1) in the validation experiments using the optimal condition for laccase production by B. rhodina MAMB-05 was in agreement with the predicted value (3.07 U mg-1), and demonstrated the fitness of the proposed model. DOI: http://dx.doi.org/10.17807/orbital.v10i7.1206 https://periodicos.ufms.br/index.php/orbital/article/view/16038Botryosphaeria rhodina MAMB-05laccasesoybean oilglycerolresponse surface methodology |
| spellingShingle | João Paulo Sacchetto Arnaldo F. da Silva Filho Maria I. Rezende Ieda Scarminio Marco A. Z. Ayub Ellen C. Giese Robert F. H. Dekker Aneli Melo Barbosa-Dekker Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil Orbital: The Electronic Journal of Chemistry Botryosphaeria rhodina MAMB-05 laccase soybean oil glycerol response surface methodology |
| title | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_full | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_fullStr | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_full_unstemmed | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_short | Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil |
| title_sort | botryosphaeria spp as producers of laccase when cultivated on vegetable oils as sole carbon source optimizing laccase production by botryosphaeria rhodina mamb 05 on soybean oil |
| topic | Botryosphaeria rhodina MAMB-05 laccase soybean oil glycerol response surface methodology |
| url | https://periodicos.ufms.br/index.php/orbital/article/view/16038 |
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