LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional Applications
<i>Boerhavia diffusa</i> is a great tropical plant and is widely used for various traditional purposes. In the present study, we examined the influence of solvents (dichloromethane, ethyl acetate, methanol and infusion (water)) on chemical composition and biological capabilities of <i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/12/2003 |
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| author | Kouadio Ibrahime Sinan Uğur Akpulat Afaf A. Aldahish Yasemin Celik Altunoglu Mehmet Cengiz Baloğlu Dimitrina Zheleva-Dimitrova Reneta Gevrenova Devina Lobine Mohamad Fawzi Mahomoodally Ouattara Katinan Etienne Gokhan Zengin Shafi Mahmud Raffaele Capasso |
| author_facet | Kouadio Ibrahime Sinan Uğur Akpulat Afaf A. Aldahish Yasemin Celik Altunoglu Mehmet Cengiz Baloğlu Dimitrina Zheleva-Dimitrova Reneta Gevrenova Devina Lobine Mohamad Fawzi Mahomoodally Ouattara Katinan Etienne Gokhan Zengin Shafi Mahmud Raffaele Capasso |
| author_sort | Kouadio Ibrahime Sinan |
| collection | DOAJ |
| description | <i>Boerhavia diffusa</i> is a great tropical plant and is widely used for various traditional purposes. In the present study, we examined the influence of solvents (dichloromethane, ethyl acetate, methanol and infusion (water)) on chemical composition and biological capabilities of <i>B. diffusa</i>. An UHPLC-HRMS method was used to determine the chemical characterization. The biological ability was examined for antioxidant, enzyme inhibitory and anti-cancer effects. To evaluate antioxidant effects, different chemical methods (ABTS, DPPH, CUPRAC, FRAP, metal chelating and phosphomolybdenum) were applied. With regard to enzyme inhibitory properties, cholinesterases, amylase, glucosidase and tyrosinase were used. The MDA-MB-231 breast cancer cell line was chosen to determine anticancer activity. Based on the UHPLC-HRMS analysis, 37 specialized metabolites were dereplicated and identified in the studied extracts. Results revealed the presence of 15 hydroxybenzoic, hydroxycinnamic, acylquinic acids, and their glycosides, one rotenoid, seven flavonoids, 12 fatty acids and two other glycosides. Among the tested extracts, the methanol extract showed a stronger antioxidant ability compared with other extracts. The methanol extract also showed the best inhibitory effects on tyrosinase and glucosidase. In the anti-cancer evaluation, the methanol extract showed stronger anticancer effects compared with water extract. In summary, our observations can contribute to the establishment of <i>B. diffusa</i> as a potential candidate for functional applications in the preparation. |
| first_indexed | 2024-03-10T04:39:01Z |
| format | Article |
| id | doaj.art-dce6d9ef817343ee89dbaddbfdf70664 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T04:39:01Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-dce6d9ef817343ee89dbaddbfdf706642023-11-23T03:34:12ZengMDPI AGAntioxidants2076-39212021-12-011012200310.3390/antiox10122003LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional ApplicationsKouadio Ibrahime Sinan0Uğur Akpulat1Afaf A. Aldahish2Yasemin Celik Altunoglu3Mehmet Cengiz Baloğlu4Dimitrina Zheleva-Dimitrova5Reneta Gevrenova6Devina Lobine7Mohamad Fawzi Mahomoodally8Ouattara Katinan Etienne9Gokhan Zengin10Shafi Mahmud11Raffaele Capasso12Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyDepartment of Medical Biology, Faculty of Medicine, Kastamonu University, Kastamonu 37150, TurkeyDepartment of Pharmacology and Toxicology, College of Pharmacy, King Khalid University, Abha 62529, Asir, Saudi ArabiaDepartment of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu 37150, TurkeyDepartment of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University, Kastamonu 37150, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia, 1431 Soifa, BulgariaDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia, 1431 Soifa, BulgariaDepartment of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit 80837, MauritiusDepartment of Health Sciences, Faculty of Medicine and Health Sciences, University of Mauritius, Réduit 80837, MauritiusLaboratoire de Botanique, UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan 00225, Côte d’IvoirePhysiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, TurkeyGenetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy<i>Boerhavia diffusa</i> is a great tropical plant and is widely used for various traditional purposes. In the present study, we examined the influence of solvents (dichloromethane, ethyl acetate, methanol and infusion (water)) on chemical composition and biological capabilities of <i>B. diffusa</i>. An UHPLC-HRMS method was used to determine the chemical characterization. The biological ability was examined for antioxidant, enzyme inhibitory and anti-cancer effects. To evaluate antioxidant effects, different chemical methods (ABTS, DPPH, CUPRAC, FRAP, metal chelating and phosphomolybdenum) were applied. With regard to enzyme inhibitory properties, cholinesterases, amylase, glucosidase and tyrosinase were used. The MDA-MB-231 breast cancer cell line was chosen to determine anticancer activity. Based on the UHPLC-HRMS analysis, 37 specialized metabolites were dereplicated and identified in the studied extracts. Results revealed the presence of 15 hydroxybenzoic, hydroxycinnamic, acylquinic acids, and their glycosides, one rotenoid, seven flavonoids, 12 fatty acids and two other glycosides. Among the tested extracts, the methanol extract showed a stronger antioxidant ability compared with other extracts. The methanol extract also showed the best inhibitory effects on tyrosinase and glucosidase. In the anti-cancer evaluation, the methanol extract showed stronger anticancer effects compared with water extract. In summary, our observations can contribute to the establishment of <i>B. diffusa</i> as a potential candidate for functional applications in the preparation.https://www.mdpi.com/2076-3921/10/12/2003<i>Boerhavia diffusa</i>antioxidantenzyme inhibitionanti-cancerflavonoidsfunctional applications |
| spellingShingle | Kouadio Ibrahime Sinan Uğur Akpulat Afaf A. Aldahish Yasemin Celik Altunoglu Mehmet Cengiz Baloğlu Dimitrina Zheleva-Dimitrova Reneta Gevrenova Devina Lobine Mohamad Fawzi Mahomoodally Ouattara Katinan Etienne Gokhan Zengin Shafi Mahmud Raffaele Capasso LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional Applications Antioxidants <i>Boerhavia diffusa</i> antioxidant enzyme inhibition anti-cancer flavonoids functional applications |
| title | LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional Applications |
| title_full | LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional Applications |
| title_fullStr | LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional Applications |
| title_full_unstemmed | LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional Applications |
| title_short | LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of <i>Boerhavia diffusa</i> Extracts: A Potential Raw Material for Functional Applications |
| title_sort | lc ms hrms analysis anti cancer anti enzymatic and anti oxidant effects of i boerhavia diffusa i extracts a potential raw material for functional applications |
| topic | <i>Boerhavia diffusa</i> antioxidant enzyme inhibition anti-cancer flavonoids functional applications |
| url | https://www.mdpi.com/2076-3921/10/12/2003 |
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