Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm
To optimize the reproduction of Jeju black cattle (JBC), freezing conditions for sperm were established and sperm motility, vitality, morphology, and fertility were evaluated to select the optimal bull for breeding. Semen samples from five JBC bulls were individually mixed with freezing medium at a...
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MDPI AG
2022-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/5/535 |
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| author | Jae-Wook Yoon Seung-Eun Lee Won-Jae Kim Dae-Cheol Kim Cheol-Ho Hyun Shin-Ji Lee Hyo-Jin Park So-Hee Kim Seung-Hwan Oh Do-Geon Lee Da-Bin Pyeon Eun-Young Kim Se-Pill Park |
| author_facet | Jae-Wook Yoon Seung-Eun Lee Won-Jae Kim Dae-Cheol Kim Cheol-Ho Hyun Shin-Ji Lee Hyo-Jin Park So-Hee Kim Seung-Hwan Oh Do-Geon Lee Da-Bin Pyeon Eun-Young Kim Se-Pill Park |
| author_sort | Jae-Wook Yoon |
| collection | DOAJ |
| description | To optimize the reproduction of Jeju black cattle (JBC), freezing conditions for sperm were established and sperm motility, vitality, morphology, and fertility were evaluated to select the optimal bull for breeding. Semen samples from five JBC bulls were individually mixed with freezing medium at a final concentration of 1 × 108 sperm/mL and frozen in liquid nitrogen vapor at a height of 3 or 7 cm (referred to as 3 cm sperm and 7 cm sperm, respectively). When the freezing conditions were compared, the motility of 7 cm sperm was significantly higher than that of 3 cm sperm for the JBC-A bull. The motility, curvilinear velocity, straight-line velocity, and average path velocity of fresh and frozen–thawed sperm were the highest for the JBC-A bull. The vitalities of fresh and frozen–thawed sperm were the highest for the JBC-A/E and JBC-A bulls, respectively. The percentage of normal cells in fresh sperm was the highest for the JBC-D bull. The rates of the normal formation of two pronuclei and total sperm penetration were the highest in zygotes fertilized with sperm from the JBC-A bull. The sperm from the JBC-A bull had superior qualities and are thus the most appropriate choice for the preservation and reproduction of these endangered cattle. |
| first_indexed | 2024-03-09T20:51:04Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T20:51:04Z |
| publishDate | 2022-02-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-dceb6f3cc6074adea526deff0f50a3582023-11-23T22:36:01ZengMDPI AGAnimals2076-26152022-02-0112553510.3390/ani12050535Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC SpermJae-Wook Yoon0Seung-Eun Lee1Won-Jae Kim2Dae-Cheol Kim3Cheol-Ho Hyun4Shin-Ji Lee5Hyo-Jin Park6So-Hee Kim7Seung-Hwan Oh8Do-Geon Lee9Da-Bin Pyeon10Eun-Young Kim11Se-Pill Park12Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaJeju Special Self-Governing Province Livestock Promotion Agency, 13 Sinbimaeul, Jeju-si 63078, KoreaJeju Special Self-Governing Province Livestock Promotion Agency, 13 Sinbimaeul, Jeju-si 63078, KoreaJeju Special Self-Governing Province Livestock Promotion Agency, 13 Sinbimaeul, Jeju-si 63078, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaFaculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si 63243, KoreaTo optimize the reproduction of Jeju black cattle (JBC), freezing conditions for sperm were established and sperm motility, vitality, morphology, and fertility were evaluated to select the optimal bull for breeding. Semen samples from five JBC bulls were individually mixed with freezing medium at a final concentration of 1 × 108 sperm/mL and frozen in liquid nitrogen vapor at a height of 3 or 7 cm (referred to as 3 cm sperm and 7 cm sperm, respectively). When the freezing conditions were compared, the motility of 7 cm sperm was significantly higher than that of 3 cm sperm for the JBC-A bull. The motility, curvilinear velocity, straight-line velocity, and average path velocity of fresh and frozen–thawed sperm were the highest for the JBC-A bull. The vitalities of fresh and frozen–thawed sperm were the highest for the JBC-A/E and JBC-A bulls, respectively. The percentage of normal cells in fresh sperm was the highest for the JBC-D bull. The rates of the normal formation of two pronuclei and total sperm penetration were the highest in zygotes fertilized with sperm from the JBC-A bull. The sperm from the JBC-A bull had superior qualities and are thus the most appropriate choice for the preservation and reproduction of these endangered cattle.https://www.mdpi.com/2076-2615/12/5/535Jeju black cattlespermcryopreservationmotilityvitalitymorphology |
| spellingShingle | Jae-Wook Yoon Seung-Eun Lee Won-Jae Kim Dae-Cheol Kim Cheol-Ho Hyun Shin-Ji Lee Hyo-Jin Park So-Hee Kim Seung-Hwan Oh Do-Geon Lee Da-Bin Pyeon Eun-Young Kim Se-Pill Park Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm Animals Jeju black cattle sperm cryopreservation motility vitality morphology |
| title | Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm |
| title_full | Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm |
| title_fullStr | Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm |
| title_full_unstemmed | Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm |
| title_short | Evaluation of Semen Quality of Jeju Black Cattle (JBC) to Select Bulls Optimal for Breeding and Establish Freezing Conditions Suitable for JBC Sperm |
| title_sort | evaluation of semen quality of jeju black cattle jbc to select bulls optimal for breeding and establish freezing conditions suitable for jbc sperm |
| topic | Jeju black cattle sperm cryopreservation motility vitality morphology |
| url | https://www.mdpi.com/2076-2615/12/5/535 |
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