V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells
Abstract Background The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA...
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BMC
2019-07-01
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Series: | Advances in Rheumatology |
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Online Access: | http://link.springer.com/article/10.1186/s42358-019-0069-5 |
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author | Wilson de Melo Cruvinel Luis Eduardo Coelho Andrade Carlos Alberto von Mühlen Alessandra Dellavance Antônio Carlos Ximenes Carlos David Bichara Cleonice Bueno Cristóvão Luis Pitangueira Mangueira Eloísa Bonfá Fabiano de Almeida Brito Fernanda Bull Flumian Glaucielen Gomes da Silva Jozelia Rêgo Lisiane Maria Ericoni dos Anjos Natasha Slhessarenko Sandra Gofinet Pasoto Suzane Pretti Figueiredo Neves Valéria Valim Wilton Silva dos Santos Paulo Luiz Carvalho Francescantonio |
author_facet | Wilson de Melo Cruvinel Luis Eduardo Coelho Andrade Carlos Alberto von Mühlen Alessandra Dellavance Antônio Carlos Ximenes Carlos David Bichara Cleonice Bueno Cristóvão Luis Pitangueira Mangueira Eloísa Bonfá Fabiano de Almeida Brito Fernanda Bull Flumian Glaucielen Gomes da Silva Jozelia Rêgo Lisiane Maria Ericoni dos Anjos Natasha Slhessarenko Sandra Gofinet Pasoto Suzane Pretti Figueiredo Neves Valéria Valim Wilton Silva dos Santos Paulo Luiz Carvalho Francescantonio |
author_sort | Wilson de Melo Cruvinel |
collection | DOAJ |
description | Abstract Background The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA Patterns (ICAP) recommendations (www.anapatterns.org). Initial guidelines were formulated by the group of Brazilian experts with the purpose of guiding and enabling Brazilian clinical laboratories to adopt recommendations and to provide a common standard for national and international consensuses. Mainbody Twenty Brazilian researchers and experts from universities and clinical laboratories representing the various geographical regions of the country participated in the meeting. Three main topics were discussed, namely the harmonization between the BCA guidelines and latest recommendations of the ICAP initiative, the adjustment of the terminology and report on HEp-2 patterns, and a reassessment of quality assurance parameters. For the three topics, our aim was to establish specific guidelines. All recommendations were based on consensus among participants. There was concrete progress in the adjustment of the BCA guidelines to match the ICAP guidelines. To a certain extent, this derives from the fact that ICAP recommendations were largely based on the algorithm and recommendations of the IV Brazilian ANA Consensus, as consistently recognized in the ICAP publications and presentations. However, although there is great overlap between the two Consensuses, there are some point divergences. These specific items were individually and extensively discussed, and it was acknowledged that in several points ICAP improved recommendations previously issued by the Brazilian ANA Consensus and these changes were readily implemented. Regarding some specific topics, the BCA panel of experts felt that the previously issued recommendations remained relevant and possibly will require further discussion with ICAP. The term anti-cell antibodies was adopted as the recommended designation, recognizing that the assay addresses antibodies against antigens in the nucleus and in other cell compartments. However, the acronym ANA HEp-2 was maintained due to historical and regulatory reasons. It was also signalized that the latest trend in ICAP is to adopt the term Indirect Immunofluorescent Assay on HEp-2 cell substrate (HEp-2 IIFA). In addition, the quality assurance strategies previously presented were ratified and emphasized. Conclusion The V BCA edition was successful in establishing an overall harmonization with the ICAP recommendations for interpretation of the HEp-2 IIFA test, pinpointing the perspectives in filling the remaining gaps between both initiatives. |
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publisher | BMC |
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series | Advances in Rheumatology |
spelling | doaj.art-dcede9ae68854c748251e2d04121b0722022-12-22T03:53:52ZengBMCAdvances in Rheumatology2523-31062019-07-0159111110.1186/s42358-019-0069-5V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cellsWilson de Melo Cruvinel0Luis Eduardo Coelho Andrade1Carlos Alberto von Mühlen2Alessandra Dellavance3Antônio Carlos Ximenes4Carlos David Bichara5Cleonice Bueno6Cristóvão Luis Pitangueira Mangueira7Eloísa Bonfá8Fabiano de Almeida Brito9Fernanda Bull Flumian10Glaucielen Gomes da Silva11Jozelia Rêgo12Lisiane Maria Ericoni dos Anjos13Natasha Slhessarenko14Sandra Gofinet Pasoto15Suzane Pretti Figueiredo Neves16Valéria Valim17Wilton Silva dos Santos18Paulo Luiz Carvalho Francescantonio19Pontifícia Universidade Católica de Goiás (PUC Goiás), Escola de Ciências Médicas, Farmacêuticas e BiomédicasDisciplina de Reumatologia, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), Divisão de Imunologia, Fleury Medicina e SaúdeSociedade Brasileira de AutoimunidadeDivisão de Pesquisa, Inovação e Desenvolvimento, Fleury Medicina e SaúdeDiretor Clinico do Hospital Geral de Goiania Alberto RassiFaculdade de Medicina Famaz, Amaral Costa Medicina DiagnósticaLaboratórios de Investigação Médica, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (FM-USP)Departamento de Patologia Clínica e Anatomia Patológica, Hospital Israelita Albert EinsteinHospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao PauloInstituto Hermes PardiniGrupo DASAFaculdade CarajásFaculdade de Medicina, Universidade Federal de Goiás (UFG)Universidade do Sul de Santa Catarina (UNISUL) e Universidade do Vale do Itajaí (UNIVALI)Universidade Federal de Mato Grosso (UFMT) e Grupo DASA CuiabáServiço de Reumatologia e Laboratório de Autoimunidade da Divisão de Laboratório Central do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (USP)Departamento de Propedêutica Complementar da Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG)Universidade Federal do Espírito Santo (UFES)Escola Superior de Ciências da Saúde do Distrito Federal e Laboratório SabinPontifícia Universidade Católica de Goiás (PUC Goiás), Escola de Ciências Médicas, Farmacêuticas e BiomédicasAbstract Background The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA Patterns (ICAP) recommendations (www.anapatterns.org). Initial guidelines were formulated by the group of Brazilian experts with the purpose of guiding and enabling Brazilian clinical laboratories to adopt recommendations and to provide a common standard for national and international consensuses. Mainbody Twenty Brazilian researchers and experts from universities and clinical laboratories representing the various geographical regions of the country participated in the meeting. Three main topics were discussed, namely the harmonization between the BCA guidelines and latest recommendations of the ICAP initiative, the adjustment of the terminology and report on HEp-2 patterns, and a reassessment of quality assurance parameters. For the three topics, our aim was to establish specific guidelines. All recommendations were based on consensus among participants. There was concrete progress in the adjustment of the BCA guidelines to match the ICAP guidelines. To a certain extent, this derives from the fact that ICAP recommendations were largely based on the algorithm and recommendations of the IV Brazilian ANA Consensus, as consistently recognized in the ICAP publications and presentations. However, although there is great overlap between the two Consensuses, there are some point divergences. These specific items were individually and extensively discussed, and it was acknowledged that in several points ICAP improved recommendations previously issued by the Brazilian ANA Consensus and these changes were readily implemented. Regarding some specific topics, the BCA panel of experts felt that the previously issued recommendations remained relevant and possibly will require further discussion with ICAP. The term anti-cell antibodies was adopted as the recommended designation, recognizing that the assay addresses antibodies against antigens in the nucleus and in other cell compartments. However, the acronym ANA HEp-2 was maintained due to historical and regulatory reasons. It was also signalized that the latest trend in ICAP is to adopt the term Indirect Immunofluorescent Assay on HEp-2 cell substrate (HEp-2 IIFA). In addition, the quality assurance strategies previously presented were ratified and emphasized. Conclusion The V BCA edition was successful in establishing an overall harmonization with the ICAP recommendations for interpretation of the HEp-2 IIFA test, pinpointing the perspectives in filling the remaining gaps between both initiatives.http://link.springer.com/article/10.1186/s42358-019-0069-5ANAAutoantibodiesHEp-2 cellsAntinuclear antibodiesIndirect immunofluorescenceStandardization |
spellingShingle | Wilson de Melo Cruvinel Luis Eduardo Coelho Andrade Carlos Alberto von Mühlen Alessandra Dellavance Antônio Carlos Ximenes Carlos David Bichara Cleonice Bueno Cristóvão Luis Pitangueira Mangueira Eloísa Bonfá Fabiano de Almeida Brito Fernanda Bull Flumian Glaucielen Gomes da Silva Jozelia Rêgo Lisiane Maria Ericoni dos Anjos Natasha Slhessarenko Sandra Gofinet Pasoto Suzane Pretti Figueiredo Neves Valéria Valim Wilton Silva dos Santos Paulo Luiz Carvalho Francescantonio V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells Advances in Rheumatology ANA Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence Standardization |
title | V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_full | V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_fullStr | V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_full_unstemmed | V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_short | V Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_sort | v brazilian consensus guidelines for detection of anti cell autoantibodies on hep 2 cells |
topic | ANA Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence Standardization |
url | http://link.springer.com/article/10.1186/s42358-019-0069-5 |
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