Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia
Antiretroviral (ARV) therapy has dramatically reduced morbidity and mortality in human immunodeficiency virus 1 (HIV-1) infected children. However, development of ARV resistance in these children is a major public health problem due to lack of availability of and access to new drugs. This study was...
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| Format: | Article |
| Language: | English |
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300012&lng=en&tlng=en |
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| author | Suharni Mohamad Zakuan Zainy Deris Nik Khairulddin Yusoff Tg Ahmad Akram Tg Mohd Ariffin Rafidah Hanim Shueb |
| author_facet | Suharni Mohamad Zakuan Zainy Deris Nik Khairulddin Yusoff Tg Ahmad Akram Tg Mohd Ariffin Rafidah Hanim Shueb |
| author_sort | Suharni Mohamad |
| collection | DOAJ |
| description | Antiretroviral (ARV) therapy has dramatically reduced morbidity and mortality in human immunodeficiency virus 1 (HIV-1) infected children. However, development of ARV resistance in these children is a major public health problem due to lack of availability of and access to new drugs. This study was conducted in order to identify circulating HIV subtypes and recombinant forms and evaluate the drug resistance mutation patterns in 18 HIV-1 infected children failing ARV treatment in Kelantan, Malaysia. Genotyping for codon 1-99 of protease (PR) and 1-250 of reverse transcriptase (RT) were performed by polymerase chain reaction (PCR) amplification and DNA sequencing. Subsequently, these were phylogenetically analyzed to determine the subtypes. CRF33_01B (44.4%) was found to be the predominant HIV subtype, followed by B (27.8%), CRF15_01B (16.7%) and CRF01_AE (11.1%) subtypes. The most prevalent RT mutations were T215F/V/Y (66.7%), D67G/N (55.6%), K219Q/E/R (44.4%), M184V/I (38.9%), K70R/E (27.8%) and M41L (27.8%), associated with nucleoside reverse transcriptase inhibitors (NRTI) resistance; and K103N (55.6%), G190A (33.3%), and K101P/E/H (27.8%) associated with non-nucleoside reverse transcriptase inhibitors (NNRTI) resistance. The results showed a possible emergence of CRF33_01B as current predominant subtypes/circulating recombinant forms (CRFs), and a high frequency of primary mutations among HIV-1 infected children after failure of ARV therapy in Kelantan, Malaysia. |
| first_indexed | 2024-12-23T04:45:21Z |
| format | Article |
| id | doaj.art-dcee745a8c3d443b99328f689ce45cb9 |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-23T04:45:21Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-dcee745a8c3d443b99328f689ce45cb92022-12-21T17:59:40ZengElsevierBrazilian Journal of Infectious Diseases1678-439116328428810.1590/S1413-86702012000300012S1413-86702012000300012Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, MalaysiaSuharni Mohamad0Zakuan Zainy Deris1Nik Khairulddin Yusoff2Tg Ahmad Akram Tg Mohd Ariffin3Rafidah Hanim Shueb4Universiti Putra MalaysiaUniversiti Putra MalaysiaHospital Raja Perempuan Zainab IIUniversiti Putra MalaysiaUniversiti Putra MalaysiaAntiretroviral (ARV) therapy has dramatically reduced morbidity and mortality in human immunodeficiency virus 1 (HIV-1) infected children. However, development of ARV resistance in these children is a major public health problem due to lack of availability of and access to new drugs. This study was conducted in order to identify circulating HIV subtypes and recombinant forms and evaluate the drug resistance mutation patterns in 18 HIV-1 infected children failing ARV treatment in Kelantan, Malaysia. Genotyping for codon 1-99 of protease (PR) and 1-250 of reverse transcriptase (RT) were performed by polymerase chain reaction (PCR) amplification and DNA sequencing. Subsequently, these were phylogenetically analyzed to determine the subtypes. CRF33_01B (44.4%) was found to be the predominant HIV subtype, followed by B (27.8%), CRF15_01B (16.7%) and CRF01_AE (11.1%) subtypes. The most prevalent RT mutations were T215F/V/Y (66.7%), D67G/N (55.6%), K219Q/E/R (44.4%), M184V/I (38.9%), K70R/E (27.8%) and M41L (27.8%), associated with nucleoside reverse transcriptase inhibitors (NRTI) resistance; and K103N (55.6%), G190A (33.3%), and K101P/E/H (27.8%) associated with non-nucleoside reverse transcriptase inhibitors (NNRTI) resistance. The results showed a possible emergence of CRF33_01B as current predominant subtypes/circulating recombinant forms (CRFs), and a high frequency of primary mutations among HIV-1 infected children after failure of ARV therapy in Kelantan, Malaysia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300012&lng=en&tlng=enHIV-1 subtyperesistance mutationchildren |
| spellingShingle | Suharni Mohamad Zakuan Zainy Deris Nik Khairulddin Yusoff Tg Ahmad Akram Tg Mohd Ariffin Rafidah Hanim Shueb Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia Brazilian Journal of Infectious Diseases HIV-1 subtype resistance mutation children |
| title | Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia |
| title_full | Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia |
| title_fullStr | Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia |
| title_full_unstemmed | Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia |
| title_short | Assessing subtypes and drug resistance mutations among HIV-1 infected children who failed antiretroviral therapy in Kelantan, Malaysia |
| title_sort | assessing subtypes and drug resistance mutations among hiv 1 infected children who failed antiretroviral therapy in kelantan malaysia |
| topic | HIV-1 subtype resistance mutation children |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300012&lng=en&tlng=en |
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