Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIR
Background: Previous studies have revealed dexmedetomidine have potential protective effects on vital organs by inhibiting the release of inflammatory cytokines. To investigate the effects of dexmedetomidine on sepsis, especially in the initial inflammatory stage of sepsis. RAW264.7 cells were used...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024037216 |
| _version_ | 1827294015248138240 |
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| author | Qin Liu Guang-Hu Yang Nai-Zhi Wang Xin-Cheng Wang Zhao-Long Zhang Lu-Jun Qiao Wen-Juan Cui |
| author_facet | Qin Liu Guang-Hu Yang Nai-Zhi Wang Xin-Cheng Wang Zhao-Long Zhang Lu-Jun Qiao Wen-Juan Cui |
| author_sort | Qin Liu |
| collection | DOAJ |
| description | Background: Previous studies have revealed dexmedetomidine have potential protective effects on vital organs by inhibiting the release of inflammatory cytokines. To investigate the effects of dexmedetomidine on sepsis, especially in the initial inflammatory stage of sepsis. RAW264.7 cells were used as the cell model in this study to elucidate the underlying mechanisms. Methods: In this study, we conducted several assays to investigate the mechanisms of dexmedetomidine and HOTAIR in sepsis. Cell viability was assessed using the CCK-8 kit, while inflammation responses were measured using ELISA for IL-1β, IL-6, and TNF-α. Additionally, we employed qPCR, MeRIP, and RIP to further explore the underlying mechanisms. Results: Our findings indicate that dexmedetomidine treatment enhanced cell viability and reduced the production of inflammatory cytokines in LPS-treated RAW264.7 cells. Furthermore, we observed that the expression of HOTAIR was increased in LPS-treated RAW264.7 cells, which was then decreased upon dexmedetomidine pre-treatment. Further investigation demonstrated that HOTAIR could counteract the beneficial effects of dexmedetomidine on cell viability and cytokine production. Interestingly, we discovered that YTHDF1 targeted HOTAIR and was upregulated in LPS-treated RAW264.7 cells, but reduced in dexmedetomidine treatment. We also found that YTHDF1 increased HOTAIR and HOTAIR m6A levels. Conclusions: Collectively, our results suggest that dexmedetomidine downregulates HOTAIR and YTHDF1 expression, which in turn inhibits the biological behavior of LPS-treated RAW264.7 cells. This finding has potential implications for the prevention and treatment of sepsis-induced kidney injury. |
| first_indexed | 2024-04-24T13:49:49Z |
| format | Article |
| id | doaj.art-dceef744f7cb4371a503a6952550718e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:49:49Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-dceef744f7cb4371a503a6952550718e2024-04-04T05:05:35ZengElsevierHeliyon2405-84402024-03-01106e27690Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIRQin Liu0Guang-Hu Yang1Nai-Zhi Wang2Xin-Cheng Wang3Zhao-Long Zhang4Lu-Jun Qiao5Wen-Juan Cui6Intensive Care Unit, Shengli Oilfield Central Hospital, Dongying, Shandong, 257000, ChinaIntensive Care Unit, Shengli Oilfield Central Hospital, Dongying, Shandong, 257000, ChinaDepartment of Respiratory and Critical Care Medicine, Jinan Central Hospital, Jinan, Shandong, 250013, ChinaIntensive Care Unit, Shengli Oilfield Central Hospital, Dongying, Shandong, 257000, ChinaIntensive Care Unit, Shengli Oilfield Central Hospital, Dongying, Shandong, 257000, ChinaIntensive Care Unit, Shengli Oilfield Central Hospital, Dongying, Shandong, 257000, China; Corresponding author.Intensive Care Unit, Shengli Oilfield Central Hospital, Dongying, Shandong, 257000, China; Corresponding author.Background: Previous studies have revealed dexmedetomidine have potential protective effects on vital organs by inhibiting the release of inflammatory cytokines. To investigate the effects of dexmedetomidine on sepsis, especially in the initial inflammatory stage of sepsis. RAW264.7 cells were used as the cell model in this study to elucidate the underlying mechanisms. Methods: In this study, we conducted several assays to investigate the mechanisms of dexmedetomidine and HOTAIR in sepsis. Cell viability was assessed using the CCK-8 kit, while inflammation responses were measured using ELISA for IL-1β, IL-6, and TNF-α. Additionally, we employed qPCR, MeRIP, and RIP to further explore the underlying mechanisms. Results: Our findings indicate that dexmedetomidine treatment enhanced cell viability and reduced the production of inflammatory cytokines in LPS-treated RAW264.7 cells. Furthermore, we observed that the expression of HOTAIR was increased in LPS-treated RAW264.7 cells, which was then decreased upon dexmedetomidine pre-treatment. Further investigation demonstrated that HOTAIR could counteract the beneficial effects of dexmedetomidine on cell viability and cytokine production. Interestingly, we discovered that YTHDF1 targeted HOTAIR and was upregulated in LPS-treated RAW264.7 cells, but reduced in dexmedetomidine treatment. We also found that YTHDF1 increased HOTAIR and HOTAIR m6A levels. Conclusions: Collectively, our results suggest that dexmedetomidine downregulates HOTAIR and YTHDF1 expression, which in turn inhibits the biological behavior of LPS-treated RAW264.7 cells. This finding has potential implications for the prevention and treatment of sepsis-induced kidney injury.http://www.sciencedirect.com/science/article/pii/S2405844024037216m6ADexmedetomidineRAW264.7 cellsYTHDF1HOTAIR |
| spellingShingle | Qin Liu Guang-Hu Yang Nai-Zhi Wang Xin-Cheng Wang Zhao-Long Zhang Lu-Jun Qiao Wen-Juan Cui Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIR Heliyon m6A Dexmedetomidine RAW264.7 cells YTHDF1 HOTAIR |
| title | Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIR |
| title_full | Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIR |
| title_fullStr | Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIR |
| title_full_unstemmed | Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIR |
| title_short | Dexmedetomidine suppressed the biological behavior of RAW264.7 cells treated with LPS by down-regulating HOTAIR |
| title_sort | dexmedetomidine suppressed the biological behavior of raw264 7 cells treated with lps by down regulating hotair |
| topic | m6A Dexmedetomidine RAW264.7 cells YTHDF1 HOTAIR |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024037216 |
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