Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation
It is known that the primo vascular system (PVS) includes the primo nodes and vessels. However, the relevant genes in the PVS system for both pathologic and physiologic condition are poorly understood. Here, we first examined the gene expression in primo vessels (PVs) floating in lymphatic endotheli...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Medical Association of Pharmacopuncture Institute
2019-02-01
|
| Series: | Journal of Acupuncture & Meridian Studies |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2005290118300104 |
| _version_ | 1818437687233740800 |
|---|---|
| author | Jun-Young Shin Jong-Ok Ji Da-Woon Choi Sang-Heon Choi Jong-Gu Choi Min-Suk Rho Ji Yoon Lee Sang-Suk Lee |
| author_facet | Jun-Young Shin Jong-Ok Ji Da-Woon Choi Sang-Heon Choi Jong-Gu Choi Min-Suk Rho Ji Yoon Lee Sang-Suk Lee |
| author_sort | Jun-Young Shin |
| collection | DOAJ |
| description | It is known that the primo vascular system (PVS) includes the primo nodes and vessels. However, the relevant genes in the PVS system for both pathologic and physiologic condition are poorly understood. Here, we first examined the gene expression in primo vessels (PVs) floating in lymphatic endothelium by isolation of PVS and lymphatic vessels (LVs) containing PVS. To investigate therapeutic effects, both PVs and LVs containing PVS were isolated after lipopolysaccharide injection and acupuncture electric stimulation at two acupoints Joksamni (ST36) and Hapgok (LI04) following lipopolysaccharide injection. We used reverse transcriptase–polymerase chain reaction to examine expression of lymphatic endothelial cell markers and inflammatory related genes. We found that lymphatic endothelial cell markers such as fms-related tyrosine kinase 4 (Flt4), lymphatic vessel endothelial receptor (Lyve-1), prospero homeobox protein 1 (Prox-1), and podoplanin (Pdpn) were highly expressed in PV compared to that of lymphatic endothelium, suggesting pivotal roles of PV in LV under inflammation. Furthermore, lymphatic-related genes including metal-response element-binding transcription factor 2 (Mtf2), hypoxia inducible factor (Hif1a), angiotensin II type 1 receptor (Agtr1), and angiotensin II type 2 receptor (Agtr2) were also overall increased in PV, and remarkably increased and these genes except peroxisome proliferator–activated receptor gamma (Pparg) after acupuncture electric stimulation in two acupoints implying central role of PV by gene activation. Keywords: acupuncture electric stimulation, gene expression, Hapgok (LI04), Joksamni (ST36), lymph vessel, primo vessel |
| first_indexed | 2024-12-14T17:28:38Z |
| format | Article |
| id | doaj.art-dcf0f646d0d4459696b4ab83fa949d6f |
| institution | Directory Open Access Journal |
| issn | 2005-2901 |
| language | English |
| last_indexed | 2024-12-14T17:28:38Z |
| publishDate | 2019-02-01 |
| publisher | Medical Association of Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Acupuncture & Meridian Studies |
| spelling | doaj.art-dcf0f646d0d4459696b4ab83fa949d6f2022-12-21T22:53:09ZengMedical Association of Pharmacopuncture InstituteJournal of Acupuncture & Meridian Studies2005-29012019-02-01121310Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric StimulationJun-Young Shin0Jong-Ok Ji1Da-Woon Choi2Sang-Heon Choi3Jong-Gu Choi4Min-Suk Rho5Ji Yoon Lee6Sang-Suk Lee7Department of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of KoreaGoodpl Co., Ltd, Wonju, Republic of KoreaDepartment of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of KoreaDepartment of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of KoreaDepartment of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of KoreaDepartment of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of KoreaDepartment of Biomedical Science, CHA Stem Cell Institute, CHA University, Gyeonggi-do, Republic of Korea; Corresponding author. Department of Biomedical Science, CHA Stem Cell Institute, CHA University, Gyeonggi-do 11160, Republic of Korea.Department of Oriental Biomedical Engineering, Sangji University, Wonju, Republic of Korea; Corresponding author. Department of Oriental Biomedical Engineering, Sangji University, 83 Sangjidae-gil, Wonju-si, Gangwon-do 26339, Republic of Korea.It is known that the primo vascular system (PVS) includes the primo nodes and vessels. However, the relevant genes in the PVS system for both pathologic and physiologic condition are poorly understood. Here, we first examined the gene expression in primo vessels (PVs) floating in lymphatic endothelium by isolation of PVS and lymphatic vessels (LVs) containing PVS. To investigate therapeutic effects, both PVs and LVs containing PVS were isolated after lipopolysaccharide injection and acupuncture electric stimulation at two acupoints Joksamni (ST36) and Hapgok (LI04) following lipopolysaccharide injection. We used reverse transcriptase–polymerase chain reaction to examine expression of lymphatic endothelial cell markers and inflammatory related genes. We found that lymphatic endothelial cell markers such as fms-related tyrosine kinase 4 (Flt4), lymphatic vessel endothelial receptor (Lyve-1), prospero homeobox protein 1 (Prox-1), and podoplanin (Pdpn) were highly expressed in PV compared to that of lymphatic endothelium, suggesting pivotal roles of PV in LV under inflammation. Furthermore, lymphatic-related genes including metal-response element-binding transcription factor 2 (Mtf2), hypoxia inducible factor (Hif1a), angiotensin II type 1 receptor (Agtr1), and angiotensin II type 2 receptor (Agtr2) were also overall increased in PV, and remarkably increased and these genes except peroxisome proliferator–activated receptor gamma (Pparg) after acupuncture electric stimulation in two acupoints implying central role of PV by gene activation. Keywords: acupuncture electric stimulation, gene expression, Hapgok (LI04), Joksamni (ST36), lymph vessel, primo vesselhttp://www.sciencedirect.com/science/article/pii/S2005290118300104 |
| spellingShingle | Jun-Young Shin Jong-Ok Ji Da-Woon Choi Sang-Heon Choi Jong-Gu Choi Min-Suk Rho Ji Yoon Lee Sang-Suk Lee Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation Journal of Acupuncture & Meridian Studies |
| title | Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation |
| title_full | Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation |
| title_fullStr | Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation |
| title_full_unstemmed | Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation |
| title_short | Expression of Genes in Primo Vasculature Floating in Lymphatic Endothelium Under Lipopolysaccharide and Acupuncture Electric Stimulation |
| title_sort | expression of genes in primo vasculature floating in lymphatic endothelium under lipopolysaccharide and acupuncture electric stimulation |
| url | http://www.sciencedirect.com/science/article/pii/S2005290118300104 |
| work_keys_str_mv | AT junyoungshin expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation AT jongokji expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation AT dawoonchoi expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation AT sangheonchoi expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation AT jongguchoi expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation AT minsukrho expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation AT jiyoonlee expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation AT sangsuklee expressionofgenesinprimovasculaturefloatinginlymphaticendotheliumunderlipopolysaccharideandacupunctureelectricstimulation |