Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells

Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells

Summary: RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This p...

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Bibliographic Details
Main Authors: Ruchi Sharma, Devika Bose, Jair Montford, Davide Ortolan, Kapil Bharti
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Flow Cytometry/Mass Cytometry
Health Sciences
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004622

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