Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artific...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
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| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/10/2/30 |
| _version_ | 1797725366173827072 |
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| author | Erdinc Sezgin Dario Carugo Ilya Levental Eleanor Stride Christian Eggeling |
| author_facet | Erdinc Sezgin Dario Carugo Ilya Levental Eleanor Stride Christian Eggeling |
| author_sort | Erdinc Sezgin |
| collection | DOAJ |
| description | Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics. |
| first_indexed | 2024-03-12T10:30:09Z |
| format | Article |
| id | doaj.art-dcf6b92d25fa4a2ba1c80f22c798fd35 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-12T10:30:09Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-dcf6b92d25fa4a2ba1c80f22c798fd352023-09-02T09:21:47ZengMDPI AGMembranes2077-03752020-02-011023010.3390/membranes10020030membranes10020030Creating Supported Plasma Membrane Bilayers Using Acoustic PressureErdinc Sezgin0Dario Carugo1Ilya Levental2Eleanor Stride3Christian Eggeling4MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UKBioengineering Science Research Group, Faculty of Engineering and Physical Sciences, Institute for Life Sciences (IfLS), University of Southampton, SO17 1BJ Southampton, UKMcGovern Medical School, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, Houston, TX 77030, USAInstitute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UKMRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UKModel membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics.https://www.mdpi.com/2077-0375/10/2/30gpmvsacoustic pressuresupported bilayersplasma membrane vesiclesplasma membrane bilayers |
| spellingShingle | Erdinc Sezgin Dario Carugo Ilya Levental Eleanor Stride Christian Eggeling Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure Membranes gpmvs acoustic pressure supported bilayers plasma membrane vesicles plasma membrane bilayers |
| title | Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure |
| title_full | Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure |
| title_fullStr | Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure |
| title_full_unstemmed | Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure |
| title_short | Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure |
| title_sort | creating supported plasma membrane bilayers using acoustic pressure |
| topic | gpmvs acoustic pressure supported bilayers plasma membrane vesicles plasma membrane bilayers |
| url | https://www.mdpi.com/2077-0375/10/2/30 |
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