Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes
Development of a miniaturized biosensor system that can be used for rapid detection and counting of microorganisms in food or water samples is described. The developed microsystem employs a highly sensitive impedimetric array of biosensors to monitor the growth of bacterial colonies that are dispers...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-06-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/13/7/8188 |
| _version_ | 1817996767154667520 |
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| author | Behraad Bahreyni Shaoqing Tim Tan Ram Mehta Avneet Bajwa |
| author_facet | Behraad Bahreyni Shaoqing Tim Tan Ram Mehta Avneet Bajwa |
| author_sort | Behraad Bahreyni |
| collection | DOAJ |
| description | Development of a miniaturized biosensor system that can be used for rapid detection and counting of microorganisms in food or water samples is described. The developed microsystem employs a highly sensitive impedimetric array of biosensors to monitor the growth of bacterial colonies that are dispersed across an agar growth medium. To use the system, a sample containing the bacteria is cultured above the agar layer. Using a multiplexing network, the electrical properties of the medium at different locations are continuously measured, recorded, and compared against a baseline signal. Variations of signals from different biosensors are used to reveal the presence of bacteria in the sample, as well as the locations of bacterial colonies across the biochip. This technique forms the basis for a label-free bacterial detection for rapid analysis of food samples, reducing the detection time by at least a factor of four compared to the current required incubation times of 24 to 72 hours for plate count techniques. The developed microsystem has the potential for miniaturization to a stage where it could be deployed for rapid analysis of food samples at commercial scale at laboratories, food processing facilities, and retailers. |
| first_indexed | 2024-04-14T02:27:02Z |
| format | Article |
| id | doaj.art-dcf744cecee24e2ea649a4c8c304af75 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-14T02:27:02Z |
| publishDate | 2013-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-dcf744cecee24e2ea649a4c8c304af752022-12-22T02:17:50ZengMDPI AGSensors1424-82202013-06-011378188819810.3390/s130708188Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed MicroelectrodesBehraad BahreyniShaoqing Tim TanRam MehtaAvneet BajwaDevelopment of a miniaturized biosensor system that can be used for rapid detection and counting of microorganisms in food or water samples is described. The developed microsystem employs a highly sensitive impedimetric array of biosensors to monitor the growth of bacterial colonies that are dispersed across an agar growth medium. To use the system, a sample containing the bacteria is cultured above the agar layer. Using a multiplexing network, the electrical properties of the medium at different locations are continuously measured, recorded, and compared against a baseline signal. Variations of signals from different biosensors are used to reveal the presence of bacteria in the sample, as well as the locations of bacterial colonies across the biochip. This technique forms the basis for a label-free bacterial detection for rapid analysis of food samples, reducing the detection time by at least a factor of four compared to the current required incubation times of 24 to 72 hours for plate count techniques. The developed microsystem has the potential for miniaturization to a stage where it could be deployed for rapid analysis of food samples at commercial scale at laboratories, food processing facilities, and retailers.http://www.mdpi.com/1424-8220/13/7/8188biosensorimpedimetric detectionfoodborne pathogensmicroorganismsplate countbacterial colonylab-on-chip |
| spellingShingle | Behraad Bahreyni Shaoqing Tim Tan Ram Mehta Avneet Bajwa Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes Sensors biosensor impedimetric detection foodborne pathogens microorganisms plate count bacterial colony lab-on-chip |
| title | Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes |
| title_full | Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes |
| title_fullStr | Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes |
| title_full_unstemmed | Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes |
| title_short | Rapid Detection of Viable Microorganisms Based on a Plate Count Technique Using Arrayed Microelectrodes |
| title_sort | rapid detection of viable microorganisms based on a plate count technique using arrayed microelectrodes |
| topic | biosensor impedimetric detection foodborne pathogens microorganisms plate count bacterial colony lab-on-chip |
| url | http://www.mdpi.com/1424-8220/13/7/8188 |
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