ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta)
ARO4 gene encodes the enzyme 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase catalyses the first step in aromatic amino acid biosynthesis in prokaryotes, plants and fungi. Successful application of modified ARO4m gene encoding DAHP synthase insensitive to the feedback inhibition by tyrosi...
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| Format: | Article |
| Language: | English |
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Львівський національний університет імені Івана Франка
2010-09-01
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| Series: | Біологічні студії |
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| Online Access: | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/421 |
| _version_ | 1818602176497319936 |
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| author | K. V. Dmytruk |
| author_facet | K. V. Dmytruk |
| author_sort | K. V. Dmytruk |
| collection | DOAJ |
| description | ARO4 gene encodes the enzyme 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase catalyses the first step in aromatic amino acid biosynthesis in prokaryotes, plants and fungi. Successful application of modified ARO4m gene encoding DAHP synthase insensitive to the feedback inhibition by tyrosine for selection of Candida famata and Hansenula polymorpha yeasts transformants resistant to the DL-4-fluoro-phenylalanine is described in this work. Integrative plasmid harboring the marker transformed both yeasts with frequency up to 50 transformants per μg of DNA. |
| first_indexed | 2024-12-16T13:03:07Z |
| format | Article |
| id | doaj.art-dcfe1bf39fcf449da3f197eefc3841d4 |
| institution | Directory Open Access Journal |
| issn | 1996-4536 2311-0783 |
| language | English |
| last_indexed | 2024-12-16T13:03:07Z |
| publishDate | 2010-09-01 |
| publisher | Львівський національний університет імені Івана Франка |
| record_format | Article |
| series | Біологічні студії |
| spelling | doaj.art-dcfe1bf39fcf449da3f197eefc3841d42022-12-21T22:30:47ZengЛьвівський національний університет імені Івана ФранкаБіологічні студії1996-45362311-07832010-09-014251210.30970/sbi.0402.090ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta)K. V. Dmytruk0https://orcid.org/0000-0003-3661-8913Institute of Cell Biology NAS of UkraineARO4 gene encodes the enzyme 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase catalyses the first step in aromatic amino acid biosynthesis in prokaryotes, plants and fungi. Successful application of modified ARO4m gene encoding DAHP synthase insensitive to the feedback inhibition by tyrosine for selection of Candida famata and Hansenula polymorpha yeasts transformants resistant to the DL-4-fluoro-phenylalanine is described in this work. Integrative plasmid harboring the marker transformed both yeasts with frequency up to 50 transformants per μg of DNA.http://publications.lnu.edu.ua/journals/index.php/biology/article/view/421dominant selective markeryeastcandida famatahansenula polymorpha |
| spellingShingle | K. V. Dmytruk ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta) Біологічні студії dominant selective marker yeast candida famata hansenula polymorpha |
| title | ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta) |
| title_full | ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta) |
| title_fullStr | ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta) |
| title_full_unstemmed | ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta) |
| title_short | ARO4 gene – a new dominant selective marker for yeasts Candida famata (Candida flareri) and Hansenula polymorpha (Pichia angusta) |
| title_sort | aro4 gene a new dominant selective marker for yeasts candida famata candida flareri and hansenula polymorpha pichia angusta |
| topic | dominant selective marker yeast candida famata hansenula polymorpha |
| url | http://publications.lnu.edu.ua/journals/index.php/biology/article/view/421 |
| work_keys_str_mv | AT kvdmytruk aro4geneanewdominantselectivemarkerforyeastscandidafamatacandidaflareriandhansenulapolymorphapichiaangusta |