Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola.
The cultivation and genetic manipulation of Treponema denticola, a Gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. In this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficien...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3931704?pdf=render |
| _version_ | 1818849006230437888 |
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| author | Yuki Abiko Keiji Nagano Yasuo Yoshida Fuminobu Yoshimura |
| author_facet | Yuki Abiko Keiji Nagano Yasuo Yoshida Fuminobu Yoshimura |
| author_sort | Yuki Abiko |
| collection | DOAJ |
| description | The cultivation and genetic manipulation of Treponema denticola, a Gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. In this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficiency. We then analyzed proteins in a membrane fraction in T. denticola and identified 16 major membrane-associated proteins, and characterized one of them, TDE2508, whose biological function was not yet known. Although this protein, which exhibited a complex conformation, was presumably localized in the outer membrane, we did not find conclusive evidence that it was exposed on the cell surface. Intriguingly, a TDE2508-deficient mutant exhibited significantly increased biofilm formation and adherent activity on human gingival epithelial cells. However, the protein deficiency did not alter autoaggregation, coaggregation with Porphyromonas gingivalis, hemagglutination, cell surface hydrophobicity, motility, or expression of Msp which was reported to be an adherent molecule in this bacteria. In conclusion, the major membrane protein TDE2508 regulates biofilm formation and the adhesive potency of T. denticola, although the underlying mechanism remains unclear. |
| first_indexed | 2024-12-19T06:26:22Z |
| format | Article |
| id | doaj.art-dcff54a4398d40958c5d4414c05a1972 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T06:26:22Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dcff54a4398d40958c5d4414c05a19722022-12-21T20:32:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8905110.1371/journal.pone.0089051Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola.Yuki AbikoKeiji NaganoYasuo YoshidaFuminobu YoshimuraThe cultivation and genetic manipulation of Treponema denticola, a Gram-negative oral spirochaeta associated with periodontal diseases, is still challenging. In this study, we formulated a simple medium based on a commercially available one, and established a transformation method with high efficiency. We then analyzed proteins in a membrane fraction in T. denticola and identified 16 major membrane-associated proteins, and characterized one of them, TDE2508, whose biological function was not yet known. Although this protein, which exhibited a complex conformation, was presumably localized in the outer membrane, we did not find conclusive evidence that it was exposed on the cell surface. Intriguingly, a TDE2508-deficient mutant exhibited significantly increased biofilm formation and adherent activity on human gingival epithelial cells. However, the protein deficiency did not alter autoaggregation, coaggregation with Porphyromonas gingivalis, hemagglutination, cell surface hydrophobicity, motility, or expression of Msp which was reported to be an adherent molecule in this bacteria. In conclusion, the major membrane protein TDE2508 regulates biofilm formation and the adhesive potency of T. denticola, although the underlying mechanism remains unclear.http://europepmc.org/articles/PMC3931704?pdf=render |
| spellingShingle | Yuki Abiko Keiji Nagano Yasuo Yoshida Fuminobu Yoshimura Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola. PLoS ONE |
| title | Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola. |
| title_full | Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola. |
| title_fullStr | Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola. |
| title_full_unstemmed | Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola. |
| title_short | Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola. |
| title_sort | major membrane protein tde2508 regulates adhesive potency in treponema denticola |
| url | http://europepmc.org/articles/PMC3931704?pdf=render |
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