Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in mice
IntroductionBrachial plexus avulsion (BPA) injury develops frequent and intense neuropathic pain, involving in both peripheral and central nervous systems. The incidence of anxiety or depression caused by BPA-induced neuropathic pain is high, but the underlying mechanism remains unclear.MethodsWe es...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2023.1084494/full |
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| author | Jian-lei Zhang Hang Xian Rui Zhao Ceng Luo Rou-gang Xie Tong Tian Rui Cong |
| author_facet | Jian-lei Zhang Hang Xian Rui Zhao Ceng Luo Rou-gang Xie Tong Tian Rui Cong |
| author_sort | Jian-lei Zhang |
| collection | DOAJ |
| description | IntroductionBrachial plexus avulsion (BPA) injury develops frequent and intense neuropathic pain, involving in both peripheral and central nervous systems. The incidence of anxiety or depression caused by BPA-induced neuropathic pain is high, but the underlying mechanism remains unclear.MethodsWe established a BPA mice model and assessed its negative emotions through behavioral tests. To further explore the role of the microbiota-gut-brain axis in the unique emotional behavior after BPA, we performed intestinal fecal 16s and metabolomics assays. Psychobiotics (PB) supplementation was administered to BPA mice to check the probiotics effects on BPA-induced anxiety behaviors.ResultsPain related anxiety-like behavior was observed at the early stage after BPA (7 days), while no depression-like behavior was detected. Intriguingly, gut microbiota diversity was increased in BPA mice, and the most abundant probiotics, Lactobacillus, showed obvious changes. Lactobacillus_reuteri was significantly decreased in BPA mice. Metabolomics analysis showed that Lactobacillus_reuteri-related bile acid pathway and some neurotransmitter amino acids were significantly altered. Further PB (dominated by Lactobacillus_reuteri) supplementation could significantly relieve BPA-induced anxiety-like behaviors in mice.ConclusionOur study suggests that pathological neuralgia after BPA could alter intestinal microbiota diversity, especially Lactobacillus, and the changes in neurotransmitter amino acid metabolites may be the key reason for the onset of anxiety-like behaviors in BPA mice. |
| first_indexed | 2024-04-10T16:42:40Z |
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| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-04-10T16:42:40Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-dd01bf95bb0644ea8ff369bf5d107b522023-02-08T06:09:31ZengFrontiers Media S.A.Frontiers in Neurology1664-22952023-02-011410.3389/fneur.2023.10844941084494Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in miceJian-lei Zhang0Hang Xian1Rui Zhao2Ceng Luo3Rou-gang Xie4Tong Tian5Rui Cong6Department of Orthopedics, Xijing Hospital, Air Force Medical University, Xi'an, ChinaDepartment of Orthopedics, Xijing Hospital, Air Force Medical University, Xi'an, ChinaDepartment of Orthopedics, Xijing Hospital, Air Force Medical University, Xi'an, ChinaDepartment of Neurobiology, School of Basic Medicine, Air Force Medical University, Xi'an, ChinaDepartment of Neurobiology, School of Basic Medicine, Air Force Medical University, Xi'an, ChinaDepartment of Orthopedics, Xijing Hospital, Air Force Medical University, Xi'an, ChinaDepartment of Orthopedics, Xijing Hospital, Air Force Medical University, Xi'an, ChinaIntroductionBrachial plexus avulsion (BPA) injury develops frequent and intense neuropathic pain, involving in both peripheral and central nervous systems. The incidence of anxiety or depression caused by BPA-induced neuropathic pain is high, but the underlying mechanism remains unclear.MethodsWe established a BPA mice model and assessed its negative emotions through behavioral tests. To further explore the role of the microbiota-gut-brain axis in the unique emotional behavior after BPA, we performed intestinal fecal 16s and metabolomics assays. Psychobiotics (PB) supplementation was administered to BPA mice to check the probiotics effects on BPA-induced anxiety behaviors.ResultsPain related anxiety-like behavior was observed at the early stage after BPA (7 days), while no depression-like behavior was detected. Intriguingly, gut microbiota diversity was increased in BPA mice, and the most abundant probiotics, Lactobacillus, showed obvious changes. Lactobacillus_reuteri was significantly decreased in BPA mice. Metabolomics analysis showed that Lactobacillus_reuteri-related bile acid pathway and some neurotransmitter amino acids were significantly altered. Further PB (dominated by Lactobacillus_reuteri) supplementation could significantly relieve BPA-induced anxiety-like behaviors in mice.ConclusionOur study suggests that pathological neuralgia after BPA could alter intestinal microbiota diversity, especially Lactobacillus, and the changes in neurotransmitter amino acid metabolites may be the key reason for the onset of anxiety-like behaviors in BPA mice.https://www.frontiersin.org/articles/10.3389/fneur.2023.1084494/fullbrachial plexus avulsionmicrobiota-gut-brain axismetabolitesanxietypsychobiotics |
| spellingShingle | Jian-lei Zhang Hang Xian Rui Zhao Ceng Luo Rou-gang Xie Tong Tian Rui Cong Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in mice Frontiers in Neurology brachial plexus avulsion microbiota-gut-brain axis metabolites anxiety psychobiotics |
| title | Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in mice |
| title_full | Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in mice |
| title_fullStr | Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in mice |
| title_full_unstemmed | Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in mice |
| title_short | Brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety-like behavior in mice |
| title_sort | brachial plexus avulsion induced changes in gut microbiota promotes pain related anxiety like behavior in mice |
| topic | brachial plexus avulsion microbiota-gut-brain axis metabolites anxiety psychobiotics |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2023.1084494/full |
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