Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth.
The primary purpose of this time-lapse data analysis was to identify the association between the nucleation status of a Day 2 preimplantation embryo and live births following in vitro fertilization (IVF). The retrospective data analysis was based on 2769 transferred embryos from 1966 treatment cycle...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0274502 |
| _version_ | 1811264477862035456 |
|---|---|
| author | Shabana Sayed Marte Myhre Reigstad Bjørn Molt Petersen Arne Schwennicke Jon Wegner Hausken Ritsa Storeng |
| author_facet | Shabana Sayed Marte Myhre Reigstad Bjørn Molt Petersen Arne Schwennicke Jon Wegner Hausken Ritsa Storeng |
| author_sort | Shabana Sayed |
| collection | DOAJ |
| description | The primary purpose of this time-lapse data analysis was to identify the association between the nucleation status of a Day 2 preimplantation embryo and live births following in vitro fertilization (IVF). The retrospective data analysis was based on 2769 transferred embryos from 1966 treatment cycles and utilised only Known Implantation Data (KID) for live births. Nucleation errors (NE) such as micronucleation, binucleation, multinucleation and minor error groups, were annotated in the time-lapse images which were taken every 15 minutes for a minimum of 44 hours post insemination. Further, factors that may impact NE and the relationship of early morphological attributes and morphokinetic variables with NE occurrence were explored. The frequency of NE among the transferred embryos was 23.8%. The reversibility of NE evidenced by their presence at the two-cell stage, but absence at the four-cell stage was 89.6%. Embryos exhibiting nucleation errors at the two-cell stage had significantly lower live birth rates compared to embryos with no nucleation errors, constituting a significant predictor. A Generalized Additive Mixed Model was used to control for confounders and for controlling clustering effects from dual embryo transfers. Increased incidences of NE were observed with increasing age, with delayed occurrence of cell divisions and in oocytes inseminated with surgically retrieved spermatozoa. NE assessment and their impact on live birth provides valuable markers for early preimplantation embryo selection. In addition, the high incidence of reversibility of NE and their possible impact on live birth suggest that incorporating two-cell nuclear status annotations in embryo selection, alongside morphology and morphokinetics, is of value. |
| first_indexed | 2024-04-12T20:05:01Z |
| format | Article |
| id | doaj.art-dd0331306f0c4b5ca676bc6a7e6d2fe7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T20:05:01Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dd0331306f0c4b5ca676bc6a7e6d2fe72022-12-22T03:18:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179e027450210.1371/journal.pone.0274502Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth.Shabana SayedMarte Myhre ReigstadBjørn Molt PetersenArne SchwennickeJon Wegner HauskenRitsa StorengThe primary purpose of this time-lapse data analysis was to identify the association between the nucleation status of a Day 2 preimplantation embryo and live births following in vitro fertilization (IVF). The retrospective data analysis was based on 2769 transferred embryos from 1966 treatment cycles and utilised only Known Implantation Data (KID) for live births. Nucleation errors (NE) such as micronucleation, binucleation, multinucleation and minor error groups, were annotated in the time-lapse images which were taken every 15 minutes for a minimum of 44 hours post insemination. Further, factors that may impact NE and the relationship of early morphological attributes and morphokinetic variables with NE occurrence were explored. The frequency of NE among the transferred embryos was 23.8%. The reversibility of NE evidenced by their presence at the two-cell stage, but absence at the four-cell stage was 89.6%. Embryos exhibiting nucleation errors at the two-cell stage had significantly lower live birth rates compared to embryos with no nucleation errors, constituting a significant predictor. A Generalized Additive Mixed Model was used to control for confounders and for controlling clustering effects from dual embryo transfers. Increased incidences of NE were observed with increasing age, with delayed occurrence of cell divisions and in oocytes inseminated with surgically retrieved spermatozoa. NE assessment and their impact on live birth provides valuable markers for early preimplantation embryo selection. In addition, the high incidence of reversibility of NE and their possible impact on live birth suggest that incorporating two-cell nuclear status annotations in embryo selection, alongside morphology and morphokinetics, is of value.https://doi.org/10.1371/journal.pone.0274502 |
| spellingShingle | Shabana Sayed Marte Myhre Reigstad Bjørn Molt Petersen Arne Schwennicke Jon Wegner Hausken Ritsa Storeng Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth. PLoS ONE |
| title | Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth. |
| title_full | Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth. |
| title_fullStr | Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth. |
| title_full_unstemmed | Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth. |
| title_short | Nucleation status of Day 2 pre-implantation embryos, acquired by time-lapse imaging during IVF, is associated with live birth. |
| title_sort | nucleation status of day 2 pre implantation embryos acquired by time lapse imaging during ivf is associated with live birth |
| url | https://doi.org/10.1371/journal.pone.0274502 |
| work_keys_str_mv | AT shabanasayed nucleationstatusofday2preimplantationembryosacquiredbytimelapseimagingduringivfisassociatedwithlivebirth AT martemyhrereigstad nucleationstatusofday2preimplantationembryosacquiredbytimelapseimagingduringivfisassociatedwithlivebirth AT bjørnmoltpetersen nucleationstatusofday2preimplantationembryosacquiredbytimelapseimagingduringivfisassociatedwithlivebirth AT arneschwennicke nucleationstatusofday2preimplantationembryosacquiredbytimelapseimagingduringivfisassociatedwithlivebirth AT jonwegnerhausken nucleationstatusofday2preimplantationembryosacquiredbytimelapseimagingduringivfisassociatedwithlivebirth AT ritsastoreng nucleationstatusofday2preimplantationembryosacquiredbytimelapseimagingduringivfisassociatedwithlivebirth |