The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved]
Background: Pocillopora acuta is a hermatypic coral with strong ecological importance. Anthropogenic disturbances and global warming are major threats that can induce coral bleaching, the disruption of the mutualistic symbiosis between the coral host and its endosymbiotic algae. Previous works have...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wellcome
2022-01-01
|
| Series: | Wellcome Open Research |
| Subjects: | |
| Online Access: | https://wellcomeopenresearch.org/articles/6-195/v2 |
| _version_ | 1819236802674819072 |
|---|---|
| author | Patrick Masanet Christoph Grunau Pascal Romans Jeremie Vidal-Dupiol Céline Cosseau Guillaume Mitta Kelly Brener Raffalli David Roquis |
| author_facet | Patrick Masanet Christoph Grunau Pascal Romans Jeremie Vidal-Dupiol Céline Cosseau Guillaume Mitta Kelly Brener Raffalli David Roquis |
| author_sort | Patrick Masanet |
| collection | DOAJ |
| description | Background: Pocillopora acuta is a hermatypic coral with strong ecological importance. Anthropogenic disturbances and global warming are major threats that can induce coral bleaching, the disruption of the mutualistic symbiosis between the coral host and its endosymbiotic algae. Previous works have shown that somaclonal colonies display different levels of survival depending on the environmental conditions they previously faced. Epigenetic mechanisms are good candidates to explain this phenomenon. However, almost no work had been published on the P. acuta epigenome, especially on histone modifications. In this study, we aim at providing the first insight into chromatin structure of this species. Methods: We aligned the amino acid sequence of P. acuta core histones with histone sequences from various phyla. We developed a centri-filtration on sucrose gradient to separate chromatin from the host and the symbiont. The presence of histone H3 protein and specific histone modifications were then detected by western blot performed on histone extraction done from bleached and healthy corals. Finally, micrococcal nuclease (MNase) digestions were undertaken to study nucleosomal organization. Results: The centri-filtration enabled coral chromatin isolation with less than 2% of contamination by endosymbiont material. Histone sequences alignments with other species show that P. acuta displays on average ~90% of sequence similarities with mice and ~96% with other corals. H3 detection by western blot showed that H3 is clipped in healthy corals while it appeared to be intact in bleached corals. MNase treatment failed to provide the usual mononucleosomal digestion, a feature shared with some cnidarian, but not all; suggesting an unusual chromatin structure. Conclusions: These results provide a first insight into the chromatin, nucleosome and histone structure of P. acuta. The unusual patterns highlighted in this study and partly shared with other cnidarian will need to be further studied to better understand its role in corals. |
| first_indexed | 2024-12-23T13:10:14Z |
| format | Article |
| id | doaj.art-dd046b7c430b49e485390dc547131b36 |
| institution | Directory Open Access Journal |
| issn | 2398-502X |
| language | English |
| last_indexed | 2024-12-23T13:10:14Z |
| publishDate | 2022-01-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj.art-dd046b7c430b49e485390dc547131b362022-12-21T17:45:46ZengWellcomeWellcome Open Research2398-502X2022-01-01619483The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved]Patrick Masanet0Christoph Grunau1https://orcid.org/0000-0002-2161-412XPascal Romans2Jeremie Vidal-Dupiol3Céline Cosseau4Guillaume Mitta5Kelly Brener Raffalli6David Roquis7Aquarium de Canet-en-Roussillon, Canet-en-Roussillon, FranceIHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, FranceObservatoire Océanologique de Banyuls, Paris, FranceIHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, FranceIHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, FranceIHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, FranceIHPE, Univ. Montpellier, CNRS, Ifremer, Univ. Perpignan Via Domitia, Montpellier, FranceAgroscope, Nyon, 1260, SwitzerlandBackground: Pocillopora acuta is a hermatypic coral with strong ecological importance. Anthropogenic disturbances and global warming are major threats that can induce coral bleaching, the disruption of the mutualistic symbiosis between the coral host and its endosymbiotic algae. Previous works have shown that somaclonal colonies display different levels of survival depending on the environmental conditions they previously faced. Epigenetic mechanisms are good candidates to explain this phenomenon. However, almost no work had been published on the P. acuta epigenome, especially on histone modifications. In this study, we aim at providing the first insight into chromatin structure of this species. Methods: We aligned the amino acid sequence of P. acuta core histones with histone sequences from various phyla. We developed a centri-filtration on sucrose gradient to separate chromatin from the host and the symbiont. The presence of histone H3 protein and specific histone modifications were then detected by western blot performed on histone extraction done from bleached and healthy corals. Finally, micrococcal nuclease (MNase) digestions were undertaken to study nucleosomal organization. Results: The centri-filtration enabled coral chromatin isolation with less than 2% of contamination by endosymbiont material. Histone sequences alignments with other species show that P. acuta displays on average ~90% of sequence similarities with mice and ~96% with other corals. H3 detection by western blot showed that H3 is clipped in healthy corals while it appeared to be intact in bleached corals. MNase treatment failed to provide the usual mononucleosomal digestion, a feature shared with some cnidarian, but not all; suggesting an unusual chromatin structure. Conclusions: These results provide a first insight into the chromatin, nucleosome and histone structure of P. acuta. The unusual patterns highlighted in this study and partly shared with other cnidarian will need to be further studied to better understand its role in corals.https://wellcomeopenresearch.org/articles/6-195/v2Pocillopora acuta Pocillopora damicornis invertebrate epigenetics chromatin structure Histone H3 clippingeng |
| spellingShingle | Patrick Masanet Christoph Grunau Pascal Romans Jeremie Vidal-Dupiol Céline Cosseau Guillaume Mitta Kelly Brener Raffalli David Roquis The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved] Wellcome Open Research Pocillopora acuta Pocillopora damicornis invertebrate epigenetics chromatin structure Histone H3 clipping eng |
| title | The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved] |
| title_full | The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved] |
| title_fullStr | The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved] |
| title_full_unstemmed | The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved] |
| title_short | The tropical coral Pocillopora acuta displays an unusual chromatin structure and shows histone H3 clipping plasticity upon bleaching [version 2; peer review: 2 approved] |
| title_sort | tropical coral pocillopora acuta displays an unusual chromatin structure and shows histone h3 clipping plasticity upon bleaching version 2 peer review 2 approved |
| topic | Pocillopora acuta Pocillopora damicornis invertebrate epigenetics chromatin structure Histone H3 clipping eng |
| url | https://wellcomeopenresearch.org/articles/6-195/v2 |
| work_keys_str_mv | AT patrickmasanet thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT christophgrunau thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT pascalromans thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT jeremievidaldupiol thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT celinecosseau thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT guillaumemitta thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT kellybrenerraffalli thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT davidroquis thetropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT patrickmasanet tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT christophgrunau tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT pascalromans tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT jeremievidaldupiol tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT celinecosseau tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT guillaumemitta tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT kellybrenerraffalli tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved AT davidroquis tropicalcoralpocilloporaacutadisplaysanunusualchromatinstructureandshowshistoneh3clippingplasticityuponbleachingversion2peerreview2approved |