Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria
Background and Objectives: AmpC-producing Gram-negative bacterial (GNB) pathogens are distributed worldwide, especially in clinical settings. This study aimed to determine the antibiogram and the type of AmpC-β-lactamase gene harboured by GNB pathogens implicated in chronic suppurative otitis media...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-11-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4046 |
| _version_ | 1797394715021148160 |
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| author | Ibiam Francis Amadi Obasikene Catherine Nchedo Ariom Thaddaeus Obaji Monday Agbonifo Egwu Eze Iroha Chidinma Stacy Moses Ikechukwu Benjamin Iroha Ifeanyichukwu Romanus |
| author_facet | Ibiam Francis Amadi Obasikene Catherine Nchedo Ariom Thaddaeus Obaji Monday Agbonifo Egwu Eze Iroha Chidinma Stacy Moses Ikechukwu Benjamin Iroha Ifeanyichukwu Romanus |
| author_sort | Ibiam Francis Amadi |
| collection | DOAJ |
| description |
Background and Objectives: AmpC-producing Gram-negative bacterial (GNB) pathogens are distributed worldwide, especially in clinical settings. This study aimed to determine the antibiogram and the type of AmpC-β-lactamase gene harboured by GNB pathogens implicated in chronic suppurative otitis media (CSOM) cases.
Materials and Methods: Ear swab samples (300) collected from patients with active CSOM were analysed using standard microbiological techniques. Phenotypic and molecular detection of AmpC β-lactamase production was done by cefoxitin/cloxacillin double-disk synergy test and PCR respectively. Antibiogram was determined by disk diffusion technique.
Results: Among the GNB pathogens isolated from CSOM patients, P. aeruginosa was the most predominant (36.3%); followed by K. pneumoniae (22.3%), and E. coli (13.7%). Patients with active CSOM showed increased bacteria isolation rate from bilateral ear discharges than unilateral ear discharges. E. coli and P. aeruginosa were more prevalent among patients with duration of discharge >2 weeks; recording 9.0% and 20.3% respectively. AmpC β-lactamase producers accounted for 14.0%; they were highly resistant (60%-100%) to cephalosporins, trimethoprim-sulfamethoxazole, ofloxacin, amoxicillin, and tetracycline, but very susceptible (70.4%-100%) to ciprofloxacin, imipenem, and amikacin. Multiple antibiotic resistance indices of isolates ranged from 0.7-0.8. FOX-AmpC-β-lactamase gene was detected in 3.9% of the isolates.
Conclusion: The detection of AmpC β-lactamase-producing multidrug-resistant GNB pathogens harbouring FOX-AmpC-β-lactamase gene among patients with CSOM infections in our study is a serious public health problem which needs urgent intervention.
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| first_indexed | 2024-03-09T00:23:45Z |
| format | Article |
| id | doaj.art-dd0549d0f0e7420ea9abfe5b2568b278 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-09T00:23:45Z |
| publishDate | 2023-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-dd0549d0f0e7420ea9abfe5b2568b2782023-12-12T04:53:11ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-11-0115610.18502/ijm.v15i6.14139Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in NigeriaIbiam Francis Amadi0Obasikene Catherine Nchedo1Ariom Thaddaeus Obaji2Monday Agbonifo3Egwu Eze4Iroha Chidinma Stacy5Moses Ikechukwu Benjamin6Iroha Ifeanyichukwu Romanus7Department of Otorhinolaryngology, Alex Ekwueme Federal University, Ndufu Alike Ikwo, NigeriaDepartment of Radiology, Nnamdi Azikiwe University, Awka, NigeriaDepartment of Microbiology, Institute of Food Security, Environmental Resources and Agricultural Research, Federal University of Agriculture, Abeokuta, Ogun State, NigeriaDepartment of Otorhinolaryngology, Ambrose Ali University, Ekpoma, NigeriaDepartment of Medical Laboratory Science, Alex Ekwueme Federal University, Teaching Hospital Abakaliki, NigeriaDepartment of Pharmacy, Alex Ekwueme Federal University, Teaching Hospital, Abakaliki, NigeriaDepartment of Applied Microbiology, Faculty of Sciences, Ebonyi State University, Abakaliki, NigeriaDepartment of Applied Microbiology, Faculty of Sciences, Ebonyi State University, Abakaliki, Nigeria Background and Objectives: AmpC-producing Gram-negative bacterial (GNB) pathogens are distributed worldwide, especially in clinical settings. This study aimed to determine the antibiogram and the type of AmpC-β-lactamase gene harboured by GNB pathogens implicated in chronic suppurative otitis media (CSOM) cases. Materials and Methods: Ear swab samples (300) collected from patients with active CSOM were analysed using standard microbiological techniques. Phenotypic and molecular detection of AmpC β-lactamase production was done by cefoxitin/cloxacillin double-disk synergy test and PCR respectively. Antibiogram was determined by disk diffusion technique. Results: Among the GNB pathogens isolated from CSOM patients, P. aeruginosa was the most predominant (36.3%); followed by K. pneumoniae (22.3%), and E. coli (13.7%). Patients with active CSOM showed increased bacteria isolation rate from bilateral ear discharges than unilateral ear discharges. E. coli and P. aeruginosa were more prevalent among patients with duration of discharge >2 weeks; recording 9.0% and 20.3% respectively. AmpC β-lactamase producers accounted for 14.0%; they were highly resistant (60%-100%) to cephalosporins, trimethoprim-sulfamethoxazole, ofloxacin, amoxicillin, and tetracycline, but very susceptible (70.4%-100%) to ciprofloxacin, imipenem, and amikacin. Multiple antibiotic resistance indices of isolates ranged from 0.7-0.8. FOX-AmpC-β-lactamase gene was detected in 3.9% of the isolates. Conclusion: The detection of AmpC β-lactamase-producing multidrug-resistant GNB pathogens harbouring FOX-AmpC-β-lactamase gene among patients with CSOM infections in our study is a serious public health problem which needs urgent intervention. https://ijm.tums.ac.ir/index.php/ijm/article/view/4046AmpC-β-lactamase;Chronic suppurative otitis media;Gram-negative bacterial pathogens;FOX-AmpC gene;Multidrug resistance |
| spellingShingle | Ibiam Francis Amadi Obasikene Catherine Nchedo Ariom Thaddaeus Obaji Monday Agbonifo Egwu Eze Iroha Chidinma Stacy Moses Ikechukwu Benjamin Iroha Ifeanyichukwu Romanus Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria Iranian Journal of Microbiology AmpC-β-lactamase; Chronic suppurative otitis media; Gram-negative bacterial pathogens; FOX-AmpC gene; Multidrug resistance |
| title | Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria |
| title_full | Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria |
| title_fullStr | Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria |
| title_full_unstemmed | Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria |
| title_short | Detection of FOX-AmpC-β-lactamase gene and antibiogram of AmpC-beta-lactamase-producing pathogens isolated from chronic suppurative otitis media patients in Nigeria |
| title_sort | detection of fox ampc β lactamase gene and antibiogram of ampc beta lactamase producing pathogens isolated from chronic suppurative otitis media patients in nigeria |
| topic | AmpC-β-lactamase; Chronic suppurative otitis media; Gram-negative bacterial pathogens; FOX-AmpC gene; Multidrug resistance |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4046 |
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