Dual-bionic regenerative microenvironment for peripheral nerve repair
Autologous nerve grafting serves is considered the gold standard treatment for peripheral nerve defects; however, limited availability and donor area destruction restrict its widespread clinical application. Although the performance of allogeneic decellularized nerve implants has been explored, chal...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-08-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23000403 |
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| author | Yanjun Guan Zhiqi Ren Boyao Yang Wenjing Xu Wenjun Wu Xiangling Li Tieyuan Zhang Dongdong Li Shengfeng Chen Jun Bai Xiangyu Song Zhibo Jia Xing Xiong Songlin He Chaochao Li Fanqi Meng Tong Wu Jian Zhang Xiuzhi Liu Haoye Meng Jiang Peng Yu Wang |
| author_facet | Yanjun Guan Zhiqi Ren Boyao Yang Wenjing Xu Wenjun Wu Xiangling Li Tieyuan Zhang Dongdong Li Shengfeng Chen Jun Bai Xiangyu Song Zhibo Jia Xing Xiong Songlin He Chaochao Li Fanqi Meng Tong Wu Jian Zhang Xiuzhi Liu Haoye Meng Jiang Peng Yu Wang |
| author_sort | Yanjun Guan |
| collection | DOAJ |
| description | Autologous nerve grafting serves is considered the gold standard treatment for peripheral nerve defects; however, limited availability and donor area destruction restrict its widespread clinical application. Although the performance of allogeneic decellularized nerve implants has been explored, challenges such as insufficient human donors have been a major drawback to its clinical use. Tissue-engineered neural regeneration materials have been developed over the years, and researchers have explored strategies to mimic the peripheral neural microenvironment during the design of nerve catheter grafts, namely the extracellular matrix (ECM), which includes mechanical, physical, and biochemical signals that support nerve regeneration. In this study, polycaprolactone/silk fibroin (PCL/SF)-aligned electrospun material was modified with ECM derived from human umbilical cord mesenchymal stem cells (hUMSCs), and a dual-bionic nerve regeneration material was successfully fabricated. The results indicated that the developed biomimetic material had excellent biological properties, providing sufficient anchorage for Schwann cells and subsequent axon regeneration and angiogenesis processes. Moreover, the dual-bionic material exerted a similar effect to that of autologous nerve transplantation in bridging peripheral nerve defects in rats. In conclusion, this study provides a new concept for designing neural regeneration materials, and the prepared dual-bionic repair materials have excellent auxiliary regenerative ability and further preclinical testing is warranted to evaluate its clinical application potential. |
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| id | doaj.art-dd06ac84d733454dbb3399b24c6c34b5 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2025-03-22T04:31:42Z |
| publishDate | 2023-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-dd06ac84d733454dbb3399b24c6c34b52024-04-28T03:37:54ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2023-08-0126370386Dual-bionic regenerative microenvironment for peripheral nerve repairYanjun Guan0Zhiqi Ren1Boyao Yang2Wenjing Xu3Wenjun Wu4Xiangling Li5Tieyuan Zhang6Dongdong Li7Shengfeng Chen8Jun Bai9Xiangyu Song10Zhibo Jia11Xing Xiong12Songlin He13Chaochao Li14Fanqi Meng15Tong Wu16Jian Zhang17Xiuzhi Liu18Haoye Meng19Jiang Peng20Yu Wang21Institute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Co-innovation Center of Neuroregeneration, Nantong University Nantong, Jiangsu Province, 226007, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR ChinaGraduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaHebei North University, Zhangjiakou, 075051, PR ChinaHebei North University, Zhangjiakou, 075051, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; School of Medicine, Nankai University, Tianjin, 300071, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Graduate School of Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR ChinaInstitute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Co-innovation Center of Neuroregeneration, Nantong University Nantong, Jiangsu Province, 226007, PR China; Corresponding author. Institute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China.Institute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China; Co-innovation Center of Neuroregeneration, Nantong University Nantong, Jiangsu Province, 226007, PR China; Corresponding author. Institute of Orthopedics, The Fourth Medical Center of Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No. 51 Fucheng Road, Beijing, 100048, PR China.Autologous nerve grafting serves is considered the gold standard treatment for peripheral nerve defects; however, limited availability and donor area destruction restrict its widespread clinical application. Although the performance of allogeneic decellularized nerve implants has been explored, challenges such as insufficient human donors have been a major drawback to its clinical use. Tissue-engineered neural regeneration materials have been developed over the years, and researchers have explored strategies to mimic the peripheral neural microenvironment during the design of nerve catheter grafts, namely the extracellular matrix (ECM), which includes mechanical, physical, and biochemical signals that support nerve regeneration. In this study, polycaprolactone/silk fibroin (PCL/SF)-aligned electrospun material was modified with ECM derived from human umbilical cord mesenchymal stem cells (hUMSCs), and a dual-bionic nerve regeneration material was successfully fabricated. The results indicated that the developed biomimetic material had excellent biological properties, providing sufficient anchorage for Schwann cells and subsequent axon regeneration and angiogenesis processes. Moreover, the dual-bionic material exerted a similar effect to that of autologous nerve transplantation in bridging peripheral nerve defects in rats. In conclusion, this study provides a new concept for designing neural regeneration materials, and the prepared dual-bionic repair materials have excellent auxiliary regenerative ability and further preclinical testing is warranted to evaluate its clinical application potential.http://www.sciencedirect.com/science/article/pii/S2452199X23000403ElectrospunUmbilical cord mesenchymal stem cellsExtracellular matrixPeripheral nerve regenerationTissue engineering |
| spellingShingle | Yanjun Guan Zhiqi Ren Boyao Yang Wenjing Xu Wenjun Wu Xiangling Li Tieyuan Zhang Dongdong Li Shengfeng Chen Jun Bai Xiangyu Song Zhibo Jia Xing Xiong Songlin He Chaochao Li Fanqi Meng Tong Wu Jian Zhang Xiuzhi Liu Haoye Meng Jiang Peng Yu Wang Dual-bionic regenerative microenvironment for peripheral nerve repair Bioactive Materials Electrospun Umbilical cord mesenchymal stem cells Extracellular matrix Peripheral nerve regeneration Tissue engineering |
| title | Dual-bionic regenerative microenvironment for peripheral nerve repair |
| title_full | Dual-bionic regenerative microenvironment for peripheral nerve repair |
| title_fullStr | Dual-bionic regenerative microenvironment for peripheral nerve repair |
| title_full_unstemmed | Dual-bionic regenerative microenvironment for peripheral nerve repair |
| title_short | Dual-bionic regenerative microenvironment for peripheral nerve repair |
| title_sort | dual bionic regenerative microenvironment for peripheral nerve repair |
| topic | Electrospun Umbilical cord mesenchymal stem cells Extracellular matrix Peripheral nerve regeneration Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23000403 |
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