Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cells
Abstract Testicular tumours are zoonoses that can occur in not only human, but other animals, include giant pandas. A middle‐aged male giant panda named Fufu was diagnosed with a testicular tumour and underwent surgery to remove the entire left testis. The testis was mainly composed of three substan...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.1348 |
| _version_ | 1797353770610327552 |
|---|---|
| author | Chen Yijiao An Junhui Hou Rong Liu Yuliang Wang Donghui Liu Songrui Feng Tongying |
| author_facet | Chen Yijiao An Junhui Hou Rong Liu Yuliang Wang Donghui Liu Songrui Feng Tongying |
| author_sort | Chen Yijiao |
| collection | DOAJ |
| description | Abstract Testicular tumours are zoonoses that can occur in not only human, but other animals, include giant pandas. A middle‐aged male giant panda named Fufu was diagnosed with a testicular tumour and underwent surgery to remove the entire left testis. The testis was mainly composed of three substantive parts: normal tissue on the outside, tumour tissue in the middle, and necrosis in the centre. HE stains revealed that the tumour was a seminoma. Single‐cell mRNA sequence was applied to characterise cellular states and molecular circuitries of giant panda testicular seminoma. Only germ cell markers expressed in nearly all tumour cells, and the tumour cells appeared to be the same subtype of seminoma cells. We identified four clusters with unique genes expression. They were early apoptosis cells (EAC), inactive cells (IC), active cells subcluster 1 (AC‐1) and active cells subcluster 2 (AC‐2). We utilised monocle tools and found that IC cells was in the initiation stage, and EAC was one type of terminal stage, suggesting that tumour cells may undergo apoptosis in the future. AC‐2 was another type of terminal stage, representing a group of progressive cells. Our study represents the first report to utilise scRNA‐seq to characterise the cellular states and molecular circuitries of a giant panda testicular tumour. This investigation proposes CD117 and CD30 as dependable markers for future pathologic diagnosis. Our findings also suggest that CTSV and other genes with unique expression patterns in active and progressive giant panda seminoma cells may act as early prognostic biomarkers. |
| first_indexed | 2024-03-08T13:35:50Z |
| format | Article |
| id | doaj.art-dd0a2d5260af498daf393a7f7f94b9ec |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-08T13:35:50Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-dd0a2d5260af498daf393a7f7f94b9ec2024-01-16T18:58:33ZengWileyVeterinary Medicine and Science2053-10952024-01-01101n/an/a10.1002/vms3.1348Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cellsChen Yijiao0An Junhui1Hou Rong2Liu Yuliang3Wang Donghui4Liu Songrui5Feng Tongying6Chengdu Research Base of Giant Panda Breeding Chengdu ChinaChengdu Research Base of Giant Panda Breeding Chengdu ChinaChengdu Research Base of Giant Panda Breeding Chengdu ChinaChengdu Research Base of Giant Panda Breeding Chengdu ChinaChengdu Research Base of Giant Panda Breeding Chengdu ChinaChengdu Research Base of Giant Panda Breeding Chengdu ChinaSichuan Key Laboratory of Conservation Biology for Endangered Wildlife Chengdu ChinaAbstract Testicular tumours are zoonoses that can occur in not only human, but other animals, include giant pandas. A middle‐aged male giant panda named Fufu was diagnosed with a testicular tumour and underwent surgery to remove the entire left testis. The testis was mainly composed of three substantive parts: normal tissue on the outside, tumour tissue in the middle, and necrosis in the centre. HE stains revealed that the tumour was a seminoma. Single‐cell mRNA sequence was applied to characterise cellular states and molecular circuitries of giant panda testicular seminoma. Only germ cell markers expressed in nearly all tumour cells, and the tumour cells appeared to be the same subtype of seminoma cells. We identified four clusters with unique genes expression. They were early apoptosis cells (EAC), inactive cells (IC), active cells subcluster 1 (AC‐1) and active cells subcluster 2 (AC‐2). We utilised monocle tools and found that IC cells was in the initiation stage, and EAC was one type of terminal stage, suggesting that tumour cells may undergo apoptosis in the future. AC‐2 was another type of terminal stage, representing a group of progressive cells. Our study represents the first report to utilise scRNA‐seq to characterise the cellular states and molecular circuitries of a giant panda testicular tumour. This investigation proposes CD117 and CD30 as dependable markers for future pathologic diagnosis. Our findings also suggest that CTSV and other genes with unique expression patterns in active and progressive giant panda seminoma cells may act as early prognostic biomarkers.https://doi.org/10.1002/vms3.1348giant pandareproductionseminomasingle‐cell mRNA sequencingtumour |
| spellingShingle | Chen Yijiao An Junhui Hou Rong Liu Yuliang Wang Donghui Liu Songrui Feng Tongying Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cells Veterinary Medicine and Science giant panda reproduction seminoma single‐cell mRNA sequencing tumour |
| title | Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cells |
| title_full | Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cells |
| title_fullStr | Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cells |
| title_full_unstemmed | Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cells |
| title_short | Single‐cell mRNA sequencing of giant panda (Ailuropoda melanoleuca) seminoma reveals the cellular and molecular characteristics of tumour cells |
| title_sort | single cell mrna sequencing of giant panda ailuropoda melanoleuca seminoma reveals the cellular and molecular characteristics of tumour cells |
| topic | giant panda reproduction seminoma single‐cell mRNA sequencing tumour |
| url | https://doi.org/10.1002/vms3.1348 |
| work_keys_str_mv | AT chenyijiao singlecellmrnasequencingofgiantpandaailuropodamelanoleucaseminomarevealsthecellularandmolecularcharacteristicsoftumourcells AT anjunhui singlecellmrnasequencingofgiantpandaailuropodamelanoleucaseminomarevealsthecellularandmolecularcharacteristicsoftumourcells AT hourong singlecellmrnasequencingofgiantpandaailuropodamelanoleucaseminomarevealsthecellularandmolecularcharacteristicsoftumourcells AT liuyuliang singlecellmrnasequencingofgiantpandaailuropodamelanoleucaseminomarevealsthecellularandmolecularcharacteristicsoftumourcells AT wangdonghui singlecellmrnasequencingofgiantpandaailuropodamelanoleucaseminomarevealsthecellularandmolecularcharacteristicsoftumourcells AT liusongrui singlecellmrnasequencingofgiantpandaailuropodamelanoleucaseminomarevealsthecellularandmolecularcharacteristicsoftumourcells AT fengtongying singlecellmrnasequencingofgiantpandaailuropodamelanoleucaseminomarevealsthecellularandmolecularcharacteristicsoftumourcells |