Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety.
Fungal community composition in composts of lignocellulosic wastes was assessed via 454-pyrosequencing of ITS1 libraries derived from the three major composting phases. Ascomycota represented most (93%) of the 27,987 fungal sequences. A total of 102 genera, 120 species and 222 operational taxonomic...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00164/full |
| _version_ | 1818501644074090496 |
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| author | Vidya eDe Gannes Gaius eEudoxie William James Hickey |
| author_facet | Vidya eDe Gannes Gaius eEudoxie William James Hickey |
| author_sort | Vidya eDe Gannes |
| collection | DOAJ |
| description | Fungal community composition in composts of lignocellulosic wastes was assessed via 454-pyrosequencing of ITS1 libraries derived from the three major composting phases. Ascomycota represented most (93%) of the 27,987 fungal sequences. A total of 102 genera, 120 species and 222 operational taxonomic units (> 97% similarity) were identified. Thirty genera predominated (ca. 94% of the sequences), and at the species level, sequences matching Chaetomium funicola and Fusarium oxysporum were the most abundant (26% and 12%, respectively). In all composts, fungal diversity in the mature phase exceeded that of the mesophilic phase, but there was no consistent pattern in diversity changes occurring in the thermophilic phase. Fourteen species of potential human pathogens were identified, seven of which have not been previously identified in composts. This study demonstrated that deep sequencing can elucidate fungal community diversity in composts, and that this information can have important implications for compost use and human health. |
| first_indexed | 2024-12-10T20:59:06Z |
| format | Article |
| id | doaj.art-dd0a4fdb3a584419a6c11bb494dad3d9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T20:59:06Z |
| publishDate | 2013-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dd0a4fdb3a584419a6c11bb494dad3d92022-12-22T01:33:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-06-01410.3389/fmicb.2013.0016449965Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety.Vidya eDe Gannes0Gaius eEudoxie1William James Hickey2University of the West IndiesUniversity of the West IndiesUniversity of Wisconsin-MadisonFungal community composition in composts of lignocellulosic wastes was assessed via 454-pyrosequencing of ITS1 libraries derived from the three major composting phases. Ascomycota represented most (93%) of the 27,987 fungal sequences. A total of 102 genera, 120 species and 222 operational taxonomic units (> 97% similarity) were identified. Thirty genera predominated (ca. 94% of the sequences), and at the species level, sequences matching Chaetomium funicola and Fusarium oxysporum were the most abundant (26% and 12%, respectively). In all composts, fungal diversity in the mature phase exceeded that of the mesophilic phase, but there was no consistent pattern in diversity changes occurring in the thermophilic phase. Fourteen species of potential human pathogens were identified, seven of which have not been previously identified in composts. This study demonstrated that deep sequencing can elucidate fungal community diversity in composts, and that this information can have important implications for compost use and human health.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00164/fullFungipathogensdiversityCompost454-Pyrosequencing |
| spellingShingle | Vidya eDe Gannes Gaius eEudoxie William James Hickey Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety. Frontiers in Microbiology Fungi pathogens diversity Compost 454-Pyrosequencing |
| title | Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety. |
| title_full | Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety. |
| title_fullStr | Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety. |
| title_full_unstemmed | Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety. |
| title_short | Insights into fungal communities in composts revealed by 454-pyrosequencing: Implications for human health and safety. |
| title_sort | insights into fungal communities in composts revealed by 454 pyrosequencing implications for human health and safety |
| topic | Fungi pathogens diversity Compost 454-Pyrosequencing |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00164/full |
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