Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily Cultivars
This study aimed to generate interspecific hybrids between two remote lily species, <i>L. hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i>. Reciprocal crosses were performed by conventional and cut-style pollination methods, but viable seeds we...
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MDPI AG
2022-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/3/621 |
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| author | Ji-Young Kim Ye-Su Song Jong-Kuk Na Jong-Hwa Kim |
| author_facet | Ji-Young Kim Ye-Su Song Jong-Kuk Na Jong-Hwa Kim |
| author_sort | Ji-Young Kim |
| collection | DOAJ |
| description | This study aimed to generate interspecific hybrids between two remote lily species, <i>L. hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i>. Reciprocal crosses were performed by conventional and cut-style pollination methods, but viable seeds were only obtained when <i>L. hansonii</i> was used as the female parent, indicating that unilateral incompatibility exists between the two species. In the case of immature seeds, embryos with 2~3 mm were carefully removed from testa for further in vitro culture, and they grew as normal plants. A total of 343 progenies was obtained from the crosses, and hybridity of the progenies were examined using the L9 marker, simple sequence repeat (SSR) marker, at the seedling stage and 92 were confirmed as F<sub>1</sub> hybrids. Ploidy level of 76 F<sub>1</sub> hybrid was examined and confirmed as diploid. F<sub>1</sub> hybrids exhibited intermediate morphologies of the parent in outer tepal and leaf length, but flower shape and color were similar to those of <i>L. hansonii</i>. On the other hand, F<sub>1</sub> hybrid plants showed increased flower spots, flower size, and bud numbers, which could be important signatures of the F<sub>1</sub> hybrid. This study reports the first attempt to generate an interspecific hybrid between the two species, and therefore, our results from this study would be very informative for future lily breeding. |
| first_indexed | 2024-03-09T13:55:29Z |
| format | Article |
| id | doaj.art-dd0f520cfdea4293959afe369bfa1580 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:55:29Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-dd0f520cfdea4293959afe369bfa15802023-11-30T20:44:08ZengMDPI AGAgronomy2073-43952022-03-0112362110.3390/agronomy12030621Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily CultivarsJi-Young Kim0Ye-Su Song1Jong-Kuk Na2Jong-Hwa Kim3Department of Horticulture, Kangwon National University, Chuncheon 24341, KoreaDepartment of Horticulture, Kangwon National University, Chuncheon 24341, KoreaDepartment of Controlled Agriculture, Kangwon National University, Chuncheon 24341, KoreaDepartment of Horticulture, Kangwon National University, Chuncheon 24341, KoreaThis study aimed to generate interspecific hybrids between two remote lily species, <i>L. hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i>. Reciprocal crosses were performed by conventional and cut-style pollination methods, but viable seeds were only obtained when <i>L. hansonii</i> was used as the female parent, indicating that unilateral incompatibility exists between the two species. In the case of immature seeds, embryos with 2~3 mm were carefully removed from testa for further in vitro culture, and they grew as normal plants. A total of 343 progenies was obtained from the crosses, and hybridity of the progenies were examined using the L9 marker, simple sequence repeat (SSR) marker, at the seedling stage and 92 were confirmed as F<sub>1</sub> hybrids. Ploidy level of 76 F<sub>1</sub> hybrid was examined and confirmed as diploid. F<sub>1</sub> hybrids exhibited intermediate morphologies of the parent in outer tepal and leaf length, but flower shape and color were similar to those of <i>L. hansonii</i>. On the other hand, F<sub>1</sub> hybrid plants showed increased flower spots, flower size, and bud numbers, which could be important signatures of the F<sub>1</sub> hybrid. This study reports the first attempt to generate an interspecific hybrid between the two species, and therefore, our results from this study would be very informative for future lily breeding.https://www.mdpi.com/2073-4395/12/3/621immature embryo cultureinterspecific hybridsSSR markermarker-assisted selectioncut-style pollination |
| spellingShingle | Ji-Young Kim Ye-Su Song Jong-Kuk Na Jong-Hwa Kim Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily Cultivars Agronomy immature embryo culture interspecific hybrids SSR marker marker-assisted selection cut-style pollination |
| title | Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily Cultivars |
| title_full | Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily Cultivars |
| title_fullStr | Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily Cultivars |
| title_full_unstemmed | Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily Cultivars |
| title_short | Interspecific Crossing between <i>Lilium hansonii</i> Leichtlin and <i>L. brownii</i> var. <i>colchesteri</i> for the Breeding of New Lily Cultivars |
| title_sort | interspecific crossing between i lilium hansonii i leichtlin and i l brownii i var i colchesteri i for the breeding of new lily cultivars |
| topic | immature embryo culture interspecific hybrids SSR marker marker-assisted selection cut-style pollination |
| url | https://www.mdpi.com/2073-4395/12/3/621 |
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