Selection and Identification of Novel Aptamers Specific for Clenbuterol Based on ssDNA Library Immobilized SELEX and Gold Nanoparticles Biosensor

Selection and Identification of Novel Aptamers Specific for Clenbuterol Based on ssDNA Library Immobilized SELEX and Gold Nanoparticles Biosensor

We describe a multiple combined strategy to discover novel aptamers specific for clenbuterol (CBL). An immobilized ssDNA library was used for the selection of specific aptamers using the systematic evolution of ligands by exponential enrichment (SELEX). Progress was monitored using real-time quantit...

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Bibliographic Details
Main Authors: Xixia Liu, Qi Lu, Sirui Chen, Fang Wang, Jianjun Hou, Zhenlin Xu, Chen Meng, Tianyuan Hu, Yaoyao Hou
Format: Article
Language:English
Published: MDPI AG 2018-09-01
Series:Molecules
Subjects:
clenbuterol
systematic evolution of ligands by exponential enrichment
real-time quantitative PCR
high-throughput sequencing technology
aptamers
gold nanoparticles biosensor
Online Access:http://www.mdpi.com/1420-3049/23/9/2337

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