Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth Agenesis
Non-syndromic tooth agenesis (NSTA) is one of the most common dental abnormalities. MiRNAs participated in the craniofacial and tooth development. Therefore, single nucleotide polymorphisms (SNPs) in miRNA genes may contribute to the susceptibility of non-syndromic tooth agenesis. Here, a total of 6...
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.01052/full |
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| author | Min Gu Min Gu Min Gu Xin Yu Xin Yu Liwen Fan Liwen Fan Guirong Zhu Guirong Zhu Fan Yang Fan Yang Shu Lou Shu Lou Lan Ma Yongchu Pan Yongchu Pan Yongchu Pan Lin Wang Lin Wang Lin Wang |
| author_facet | Min Gu Min Gu Min Gu Xin Yu Xin Yu Liwen Fan Liwen Fan Guirong Zhu Guirong Zhu Fan Yang Fan Yang Shu Lou Shu Lou Lan Ma Yongchu Pan Yongchu Pan Yongchu Pan Lin Wang Lin Wang Lin Wang |
| author_sort | Min Gu |
| collection | DOAJ |
| description | Non-syndromic tooth agenesis (NSTA) is one of the most common dental abnormalities. MiRNAs participated in the craniofacial and tooth development. Therefore, single nucleotide polymorphisms (SNPs) in miRNA genes may contribute to the susceptibility of non-syndromic tooth agenesis. Here, a total of 625 non-syndromic tooth agenesis cases and 1,144 healthy controls were recruited, and four miRNA SNPs (miR-146a/rs2910164, miR-196a2/rs11614913, pre-miR-605/rs2043556, pre-miR-618/rs2682818) were genotyped by the TaqMan platform. Rs2043556 showed nominal associations with risk of non-syndromic tooth agenesis (PAdd = 0.021) in the overall analysis, as well as upper lateral incisor agenesis (PAdd = 0.047) and lower incisor agenesis (PAdd = 0.049) in the subgroup analysis. Notably, its significant association with upper canine agenesis was observed (PAdd = 0.0016). Rs2043556 affected the mature of miR-605-3p and miR-605-5p while dual-luciferase report analysis indicated that MDM2 was the binding target of miR-605-5p. Our study indicated that pre-miR-605 rs2043556 was associated with risk of non-syndromic tooth agenesis. |
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| spelling | doaj.art-dd1582c09e7f44b69fb358d475607f692022-12-21T23:56:26ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-08-011110.3389/fphys.2020.01052540222Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth AgenesisMin Gu0Min Gu1Min Gu2Xin Yu3Xin Yu4Liwen Fan5Liwen Fan6Guirong Zhu7Guirong Zhu8Fan Yang9Fan Yang10Shu Lou11Shu Lou12Lan Ma13Yongchu Pan14Yongchu Pan15Yongchu Pan16Lin Wang17Lin Wang18Lin Wang19Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaDepartment of Dentistry, The Third Affiliated Hospital of Soochow University, The First People’s Hospital of Changzhou, Changzhou, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, ChinaJiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Orthodontics, Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, ChinaNon-syndromic tooth agenesis (NSTA) is one of the most common dental abnormalities. MiRNAs participated in the craniofacial and tooth development. Therefore, single nucleotide polymorphisms (SNPs) in miRNA genes may contribute to the susceptibility of non-syndromic tooth agenesis. Here, a total of 625 non-syndromic tooth agenesis cases and 1,144 healthy controls were recruited, and four miRNA SNPs (miR-146a/rs2910164, miR-196a2/rs11614913, pre-miR-605/rs2043556, pre-miR-618/rs2682818) were genotyped by the TaqMan platform. Rs2043556 showed nominal associations with risk of non-syndromic tooth agenesis (PAdd = 0.021) in the overall analysis, as well as upper lateral incisor agenesis (PAdd = 0.047) and lower incisor agenesis (PAdd = 0.049) in the subgroup analysis. Notably, its significant association with upper canine agenesis was observed (PAdd = 0.0016). Rs2043556 affected the mature of miR-605-3p and miR-605-5p while dual-luciferase report analysis indicated that MDM2 was the binding target of miR-605-5p. Our study indicated that pre-miR-605 rs2043556 was associated with risk of non-syndromic tooth agenesis.https://www.frontiersin.org/article/10.3389/fphys.2020.01052/fullmiRNAsingle nucleotide polymorphismtooth agenesismiR-605-5pMDM2p53 |
| spellingShingle | Min Gu Min Gu Min Gu Xin Yu Xin Yu Liwen Fan Liwen Fan Guirong Zhu Guirong Zhu Fan Yang Fan Yang Shu Lou Shu Lou Lan Ma Yongchu Pan Yongchu Pan Yongchu Pan Lin Wang Lin Wang Lin Wang Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth Agenesis Frontiers in Physiology miRNA single nucleotide polymorphism tooth agenesis miR-605-5p MDM2 p53 |
| title | Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth Agenesis |
| title_full | Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth Agenesis |
| title_fullStr | Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth Agenesis |
| title_full_unstemmed | Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth Agenesis |
| title_short | Genetic Variants in miRNAs Are Associated With Risk of Non-syndromic Tooth Agenesis |
| title_sort | genetic variants in mirnas are associated with risk of non syndromic tooth agenesis |
| topic | miRNA single nucleotide polymorphism tooth agenesis miR-605-5p MDM2 p53 |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.01052/full |
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