Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants
The potential of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) as a tool for accelerated genetic programs in ruminants is reviewed in this article. In sheep and goats, the LOPU-IVEP platform offers the possibility of producing more offspring from elite females, as th...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/1/216 |
| _version_ | 1797410658983084032 |
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| author | Hernan Baldassarre |
| author_facet | Hernan Baldassarre |
| author_sort | Hernan Baldassarre |
| collection | DOAJ |
| description | The potential of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) as a tool for accelerated genetic programs in ruminants is reviewed in this article. In sheep and goats, the LOPU-IVEP platform offers the possibility of producing more offspring from elite females, as the procedure is minimally invasive and can be repeated more times and more frequently in the same animals compared with conventional surgical embryo recovery. On average, ~10 and ~14 viable oocytes are recovered by LOPU from sheep and goats, respectively, which results in 3–5 transferable embryos and >50% pregnancy rate after transfer. LOPU-IVEP has also been applied to prepubertal ruminants of 2–6 months of age, including bovine and buffalo calves. In dairy cattle, the technology has gained momentum in the past few years stemming from the development of genetic marker selection that has allowed predicting the production phenotype of dairy females from shortly after birth. In Holstein calves, we obtained an average of ~22 viable oocytes and ~20% transferable blastocyst rate, followed by >50% pregnancy rate after transfer, declaring the platform ready for commercial application. The present and future of this technology are discussed with a focus on improvements and research needed. |
| first_indexed | 2024-03-09T04:33:23Z |
| format | Article |
| id | doaj.art-dd16d322ba744d698c1aa2837b39de52 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T04:33:23Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-dd16d322ba744d698c1aa2837b39de522023-12-03T13:33:14ZengMDPI AGAnimals2076-26152021-01-0111121610.3390/ani11010216Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for RuminantsHernan Baldassarre0Department of Animal Science, McGill University, Montreal, QC H3A 0G4, CanadaThe potential of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) as a tool for accelerated genetic programs in ruminants is reviewed in this article. In sheep and goats, the LOPU-IVEP platform offers the possibility of producing more offspring from elite females, as the procedure is minimally invasive and can be repeated more times and more frequently in the same animals compared with conventional surgical embryo recovery. On average, ~10 and ~14 viable oocytes are recovered by LOPU from sheep and goats, respectively, which results in 3–5 transferable embryos and >50% pregnancy rate after transfer. LOPU-IVEP has also been applied to prepubertal ruminants of 2–6 months of age, including bovine and buffalo calves. In dairy cattle, the technology has gained momentum in the past few years stemming from the development of genetic marker selection that has allowed predicting the production phenotype of dairy females from shortly after birth. In Holstein calves, we obtained an average of ~22 viable oocytes and ~20% transferable blastocyst rate, followed by >50% pregnancy rate after transfer, declaring the platform ready for commercial application. The present and future of this technology are discussed with a focus on improvements and research needed.https://www.mdpi.com/2076-2615/11/1/216laparoscopyin vitro embryohormonal stimulationsheepgoatcattle |
| spellingShingle | Hernan Baldassarre Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants Animals laparoscopy in vitro embryo hormonal stimulation sheep goat cattle |
| title | Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants |
| title_full | Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants |
| title_fullStr | Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants |
| title_full_unstemmed | Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants |
| title_short | Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants |
| title_sort | laparoscopic ovum pick up followed by in vitro embryo production and transfer in assisted breeding programs for ruminants |
| topic | laparoscopy in vitro embryo hormonal stimulation sheep goat cattle |
| url | https://www.mdpi.com/2076-2615/11/1/216 |
| work_keys_str_mv | AT hernanbaldassarre laparoscopicovumpickupfollowedbyinvitroembryoproductionandtransferinassistedbreedingprogramsforruminants |