Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.

Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins.

We developed and optimized a high-throughput project workflow to generate renewable recombinant antibodies to human proteins involved in epigenetic signalling. Three different strategies to produce phage display compatible protein antigens in bacterial systems were compared, and we found that in viv...

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Main Authors: Nan Zhong, Peter Loppnau, Alma Seitova, Mani Ravichandran, Maria Fenner, Harshika Jain, Anandi Bhattacharya, Ashley Hutchinson, Marcin Paduch, Vincent Lu, Michal Olszewski, Anthony A Kossiakoff, Evan Dowdell, Akiko Koide, Shohei Koide, Haiming Huang, Vincent Nadeem, Sachdev S Sidhu, Jack F Greenblatt, Edyta Marcon, Cheryl H Arrowsmith, Aled M Edwards, Susanne Gräslund
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4593582?pdf=render

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