Validation and calibration of a novel GEM biosensor for specific detection of Cd2+, Zn2+, and Pb2+

Validation and calibration of a novel GEM biosensor for specific detection of Cd2+, Zn2+, and Pb2+

Abstract Background In this study, we designed a novel genetic circuit sensitive to Cd2+, Zn2+ and Pb2+ by mimicking the CadA/CadR operon system mediated heavy metal homeostasis mechanism of Pseudomonas aeruginosa. The regular DNA motifs on natural operon were reconfigured and coupled with the enhan...

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Main Authors: H. M. L. P. B. Herath, W. R. M. de Silva, R. S. Dassanayake, Y. I. N. S. Gunawardene, J. R. P. Jayasingha, M. K. Gayashan, L. O. B. Afonso, K. M. N. de Silva
Format: Article
Language:English
Published: BMC 2023-12-01
Series:BMC Biotechnology
Subjects:
Microbial biosensors
Cadmium
Lead
Zinc
Genetic circuits
Heavy metal detection
Online Access:https://doi.org/10.1186/s12896-023-00820-7

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